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Method for determining chemical components in traditional Chinese medicines by liquid chromatography-mass spectrometry and application of method

A technology of liquid chromatography mass spectrometry and chemical composition, which is applied in the field of chemical composition determination in traditional Chinese medicine, can solve the problems of unstable quality of finished medicine, influence on drug efficacy, high detection cost, etc., and achieve a simple, easy, fast and high-sensitivity determination method Effect

Inactive Publication Date: 2019-05-31
QIQIHAR UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] The chemical composition of traditional Chinese medicine and its compound is complex, the sources of medicinal materials are different, and the content of main components varies greatly, which may easily lead to unstable quality of finished medicine and affect the efficacy of medicine. According to the traditional analysis method, the detected ingredients are single or few, and it takes a long time. The detection cycle, more manpower and material resources, and high detection cost cannot meet the needs of different traditional Chinese medicine compounds

Method used

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  • Method for determining chemical components in traditional Chinese medicines by liquid chromatography-mass spectrometry and application of method
  • Method for determining chemical components in traditional Chinese medicines by liquid chromatography-mass spectrometry and application of method
  • Method for determining chemical components in traditional Chinese medicines by liquid chromatography-mass spectrometry and application of method

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Embodiment 1

[0039] The liquid chromatography tandem mass spectrometry method of the present embodiment determines the chemical constituents in traditional Chinese medicine, the method comprises the following steps:

[0040] S1. Preparation of mixed standard solution:

[0041] Weigh 1.6 mg of benzoyl aconitine standard product, 1.6 mg of benzoyl neoaconitine standard product, 1.09 mg of Atractylodes lactone standard product, 1.2 mg of Atractylodes lactone standard product, and 1.2 mg of Atractylodes lactone standard product Standard 1.1mg, tangshenoside 1.3mg, liquiritin 1.6mg, liquiritin 1.2mg, 8-gingerol 2.0mg and 10-gingerol 2.2mg, add absolute ethanol respectively Dissolve and settle in the volumetric flask of 5mL, obtain 10 standard product solutions respectively, be respectively benzoyl aconitine standard product solution, benzoyl neoaconitine standard product solution, atractylodes lactone standard product solution, Atractylodes lactone II standard solution, Atractylodes lactone II...

Embodiment 2

[0058] The liquid chromatography-mass spectrometry method of the present embodiment measures the method for the chemical composition in the traditional Chinese medicine, and the method comprises the following steps:

[0059] S1. Preparation of mixed standard solution:

[0060] Weigh 1.6 mg of benzoyl aconitine standard product, 1.6 mg of benzoyl neoaconitine standard product, 1.09 mg of Atractylodes lactone standard product, 1.2 mg of Atractylodes lactone standard product, and 1.2 mg of Atractylodes lactone standard product Standard 1.1mg, tangshenoside 1.3mg, liquiritin 1.6mg, liquiritin 1.2mg, 8-gingerol 2.0mg and 10-gingerol 2.2mg, add absolute ethanol respectively Dissolve and settle in the volumetric flask of 5mL, obtain 10 standard product solutions respectively, be respectively benzoyl aconitine standard product solution, benzoyl neoaconitine standard product solution, atractylodes lactone standard product solution, Atractylodes lactone II standard solution, Atractylod...

Embodiment 3

[0074] The liquid chromatography-mass spectrometry method of the present embodiment measures the method for the chemical composition in the traditional Chinese medicine, and the method comprises the following steps:

[0075] S1. Preparation of mixed standard solution:

[0076] Weigh 1.6 mg of benzoyl aconitine standard product, 1.6 mg of benzoyl neoaconitine standard product, 1.09 mg of Atractylodes lactone standard product, 1.2 mg of Atractylodes lactone standard product, and 1.2 mg of Atractylodes lactone standard product Standard 1.1mg, tangshenoside 1.3mg, liquiritin 1.6mg, liquiritin 1.2mg, 8-gingerol 2.0mg and 10-gingerol 2.2mg, add absolute ethanol respectively Dissolve and settle in the volumetric flask of 5mL, obtain 10 standard product solutions respectively, be respectively benzoyl aconitine standard product solution, benzoyl neoaconitine standard product solution, atractylodes lactone standard product solution, Atractylodes lactone II standard solution, Atractylod...

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Abstract

The invention provides a method for determining chemical components in traditional Chinese medicines by liquid chromatography-mass spectrometry. The method comprises the steps of preparing a mixed standard solution and a tested traditional Chinese medicine solution, and carrying out sample injection determination by using a liquid chromatography and mass spectrometry combined technology; and by comparing an accurate molecular weight, retention time with the chemical components in the mixed standard solution, carrying out qualitative and quantitative determination on the corresponding chemicalcomponents in a traditional Chinese medicine extracting solution. The invention further provides application of the method, and it can determine that the traditional Chinese medicines comprise the following chemical components: one or more of benzoylaconitine, benzoylmesaconine, atractylodes macrocephala lactone I, atractylodes macrocephala lactone II, atractylodes macrocephala lactone III, codonopsis alkynyl glycoside, liquiritin, liquiritigenin, 8-gingerol and 10-gingerol. By the method, simultaneous detection and quantification of one or more of the 10 chemical components in the traditionalChinese medicines can be realized. The method has the advantages of rapid and efficient determination method, high sensitivity and simple and feasible determination method, and a methodological basisis provided for the quality control of the traditional Chinese medicines.

Description

technical field [0001] The invention belongs to the technical field of determination of chemical components in traditional Chinese medicines, and in particular relates to a method and application of liquid chromatography mass spectrometry for determining chemical components in traditional Chinese medicines. Background technique [0002] The chemical composition of traditional Chinese medicine and its compound is complex, the sources of medicinal materials are different, and the content of main components varies greatly, which may easily lead to unstable quality of finished medicine and affect the efficacy of medicine. According to the traditional analysis method, the detected ingredients are single or few, and it takes a long time. The detection cycle, more manpower and material resources, and high detection cost cannot meet the needs of different traditional Chinese medicine compounds. Contents of the invention [0003] The technical problem to be solved by the present in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/88
Inventor 辛杨
Owner QIQIHAR UNIVERSITY
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