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Composite decellularized dermal matrix biological dressing and preparation method thereof

A technology of decellularized dermis and biological dressings, applied in medical science, bandages, absorbent pads, etc., can solve the problems of poor permeability, easy infection, poor biocompatibility, etc., and achieve good mechanical properties, good application effects, and good antibacterial properties Effect

Active Publication Date: 2019-06-07
GUANGZHOU RAINHOME PHARM&TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the acellular dermal matrix (ADM) dressing prepared by the traditional method has shortcomings such as poor permeability, low antibacterial performance, and poor biocompatibility. In the treatment of various wounds such as chronic wounds and skin donor sites, there are disadvantages such as long healing period, easy scar hyperplasia, and easy infection, which seriously affect the wound healing effect

Method used

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  • Composite decellularized dermal matrix biological dressing and preparation method thereof
  • Composite decellularized dermal matrix biological dressing and preparation method thereof
  • Composite decellularized dermal matrix biological dressing and preparation method thereof

Examples

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preparation example Construction

[0029] The invention provides a preparation method of a composite acellular dermal matrix biological dressing, comprising the following steps: (1) soaking a skin sample in a degreasing agent and ultrasonically degreasing to obtain degreasing skin; the degreasing agent includes fatty alcohol polysaccharide Oxyethylene ether, nonylphenol polyoxyethylene ether, sodium dodecylsulfonate; (2) decellularized skin: After repeated freezing and thawing of defatted skin, it was placed in a compound enzyme solution and incubated overnight to obtain decellularized skin; (3 ) immersing the decellularized skin in a mixed solution of carboxymethyl chitosan, hyaluronic acid, hydroxyethyl cellulose, calcium chloride and keratan sulfate; adding dropwise an oxidized sodium alginate solution for cross-linking, namely have to.

[0030] Preferably, the degreasing agent is: a mixed solution of 18wt% to 22wt% fatty alcohol polyoxyethylene ether, 13wt% to 17wt% nonylphenol polyoxyethylene ether and 4wt...

Embodiment 1

[0039] This embodiment provides a method for preparing a composite acellular dermal matrix biological dressing, the specific steps are as follows:

[0040] (1) Preparation of required reagents

[0041] Degreasing agent: 20wt% fatty alcohol polyoxyethylene ether, 15wt% nonylphenol polyoxyethylene ether, and 5wt% sodium dodecylsulfonate are mixed at a volume ratio of 1:3:2, and a 50mL mixed solution is prepared.

[0042] Compound enzyme: 3wt% Dispase and 0.25wt% trypsin-EDTA were mixed at a volume ratio of 1:3 to prepare a 10mL mixed solution.

[0043] (2) Decellularization process

[0044] 1) Sampling: Select skin samples on both sides of the upper ridge line of pigskin, cut each piece into a size of 5cm×5cm, and clean the hair.

[0045] 2) Degreasing: Soak the above samples in 100 mL of degreasing agent and ultrasonically treat them for 3-5 hours.

[0046] 3) Decellularization: A combination of physical and chemical methods, that is, a combination of repeated freezing an...

Embodiment 2

[0057] This embodiment provides a method for preparing a composite acellular dermal matrix biological dressing. Compared with Example 1, the difference is: (1) In the preparation of the required reagent, the degreasing agent is: 20wt% fatty alcohol polyoxyethylene Ether, 15wt% nonylphenol polyoxyethylene ether, 5wt% sodium dodecylsulfonate by volume ratio 1:1:1 Mixing, configure 50mL of mixed solution; compound enzyme: 3wt% Dispase and 0.25% trypsin-EDTA in volume ratio 1:2 Mix the mixed solution of configuration 10mL; (3) in the preparation of composite biological dressing, 1) with 5wt% carboxymethyl chitosan, 10wt% hyaluronic acid, 5wt% hydroxyethyl cellulose, 1wt% calcium chloride, 6wt% keratan sulfate solution by volume ratio 1:2:2:5:3 Mix and prepare a 50mL mixed solution; 2) soak the acellular matrix sample prepared above in the solution of 1) and add dropwise 1mL 15wt% oxidized sodium alginate for cross-linking, cross-linking 20min .

[0058] All the other steps a...

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Abstract

The invention provides composite dermal matrix biological dressing and a preparation method thereof. The composite decellularized dermal matrix biological dressing is prepared through the steps of mixing fatty alcohol polyoxyethylene ether with polyoxy ethylene nonyl phenyl ether and sodium dodecyl sulfate in an appropriate proportion, defatting skin, performing decellularizing treatment on skin through complex enzymes of Dispase and Trypsin-EDTA in an appropriate proportion, performing soaking in a mixed solution of carboxymethyl chitosan, hyaluronic acid, hydroxyethyl cellulose, calcium chloride and keratan sulfate, and performing a cross-linking reaction through oxidized sodium alginate. Based on high defatting rate, the characteristic of original skin collagen content is maintained, the united synergetic action of the carboxymethyl chitosan, the hyaluronic acid, the hydroxyethyl cellulose, the calcium chloride and the keratan sulfate is used for cooperation, and the composite decellularized dermal matrix biological dressing is prepared. The composite decellularized dermal matrix biological dressing has favorable efficacy of resisting bacteria and promoting healing, can concurrently have favorable mechanical properties, is easy to tile and apply on wound surfaces and is not liable to damage.

Description

technical field [0001] The invention belongs to the field of biomedical materials, in particular to a composite acellular dermal matrix biological dressing. Background technique [0002] Acellular dermal matrix dressing is a dermal substitute that can be used for full-thickness skin defects and a medical dressing for wound healing of skin injuries such as burns. It is a natural skin that has undergone a series of treatments to remove cellular components in the epidermis and dermis. However, the dermal extracellular matrix components and its three-dimensional spatial structure are retained. Such a structure can provide a "dermal template" for skin regeneration, and induce host cell components such as fibroblasts and endothelial cells to differentiate and grow in the scaffold structure after transplantation. However, the acellular dermal matrix (ADM) dressing prepared by the traditional method has shortcomings such as poor permeability, low antibacterial performance, and poor ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/42A61L15/40A61L15/46A61L15/28A61L15/18
Inventor 黄燕飞车七石刘少辉
Owner GUANGZHOU RAINHOME PHARM&TECH CO LTD
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