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Uses of bergamot and/or vitexin and products using them

A technology of vitexin and bergamot, applied in the field of medicine, can solve the problems of no treatment of arenavirus and the urgency of developing antiviral drugs, and achieve the effect of good effect.

Active Publication Date: 2021-07-30
WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There is currently no specific drug for the treatment of arenaviruses, therefore, the development of antiviral drugs is urgent

Method used

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  • Uses of bergamot and/or vitexin and products using them
  • Uses of bergamot and/or vitexin and products using them
  • Uses of bergamot and/or vitexin and products using them

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] By transfecting a plasmid with vesicular stomatitis virus (VSV) gene and transfecting a plasmid containing LASV envelope glycoprotein (GenBankaccession number HQ688673.1), the envelope protein is constructed as arenavirus LASV envelope glycoprotein, which contains a report Genetic recombinant virus, while simulating the virus infection process, can conveniently and timely perform high-throughput screening and detection. Ten concentrations of bergamot and vitetin were respectively selected (doubling dilution starting from 25 μM) to treat Vero cells and A549 cells for 1 hour, and then LASV infected cells with MOI=0.1 for 1 hour (without LASV capsule Vero cells were infected with glycoprotein VSVpv as a control group), and the mixture of drug and virus was discarded after changing the medium. After 23 hours, the cells were lysed to measure the luciferase reading value. The results of bergamotin inhibition of LASV are as follows figure 1 As shown, the results of vitexin in...

Embodiment 2

[0062] With reference to the test method in Example 1, construct recombinant viruses of other viruses of the Arenaviridae family, and the Genebank numbers are as follows: LUJV (NC_012776.1), MOPV (AY772170.1), GTOV (NC_005077.1), JUNV (strainXJ13, NC_005081 .1), MACV (strain Carvallo, NC_005078.1), SABV (U41071.1), CHPV (NC_010562.1), EBOV (strain Mayinga, EU224440.2), and MARV (YP_001531156.1).

[0063] With reference to the test operation in Example 1, the inhibitory effect of bergamot on the above-mentioned Arenaviridae virus is detected, and the results are as follows: image 3 (GTOVpv, IC 50 =1.516μM), Figure 4 (JNUVpv, IC 50 =3.374μM), Figure 5 (MACVpv, IC 50 =11.7μM), Image 6 (SABVpv, IC 50 =3.861μM), Figure 7 (CHPVpv, IC 50 =5.876μM), it can be seen that bergamot also has inhibitory effect on JUNV, MACV, GTOV, CHPA and SABV infection.

[0064] With reference to the test operation in Example 1, detect the inhibitory effect of vitexin to the above-mentioned ...

Embodiment 3

[0066] The cytotoxicity of bergamot and vitexin was detected, and the cell viability was measured by MTT method.

[0067] MTT can be reduced by intracellular mitochondrial succinate dehydrogenase to form crystalline formazan, which is insoluble in water but readily soluble in DMSO. After dissolving in DMSO, the absorbance at 492nm can be detected with a microplate reader to quantify this substance. The activity of succinate dehydrogenase reflects the activity of cells, so the detection of the amount of formazan can reflect the activity of cells. Three concentrations of bergamot and vitexin were respectively set at 100 μM, 50 μM, and 25 μM. The specific steps of the method are as follows:

[0068] 1) Spread Vero cells into a 96-well plate with a cell density of 1.0×10 5 individual / mL.

[0069] 2) Add serially diluted inhibitors bergamotin or vitexin (diluted in DMEM medium containing 2% FBS) to the cells and incubate for 24 hours.

[0070] 3) Discard the cell supernatant, a...

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Abstract

The invention relates to the technical field of medicine, in particular, it provides an application of bergamot and / or vitexin and products using the same. The invention provides the application of bergamotin and / or vitexin in the preparation of products for preventing and / or treating arenaviridae virus infection, both bergamot and viticin are small molecular compounds of traditional Chinese medicine monomers, which are used in In the range of non-toxicity, it can effectively inhibit the infection of arenaviridae virus to cells, and fills the blank of current lack of treatment for arenaviridae virus infection. Products prepared with bergamot and / or vitexin as active ingredients include medicines and functional foods, and have good effects on the prevention and treatment of arenaviridae virus infection.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to an application of bergamot and / or vitexin and a product using the same. Background technique [0002] The Arenaviridae Mammalian Arenavirus genus includes more than 30 species of viruses, which can be divided into Old World virus and New World virus according to virus antigenicity, serotype and geographical distribution. Both Lassa virus (LASV) and Lujo virus (LUJV) in the old world cause severe infectious hemorrhagic disease with a high fatality rate, and are listed as Class A bioterrorism pathogens by the US CDC. The causative viruses in the mammalian arenavirus genus also include the New World Junín virus (JUNV), Machupo virus (Machupo virus, MACV), Guanarito virus (Guanarito virus, GTOV), sand Biya virus (Sabiávirus, SABV), Chaparevirus (Chaparevirus, CHPA). The above-mentioned viruses, like Lassa virus, are level 4 pathogens and pose a huge threat to human safety. [000...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/37A61K31/352A61P31/14A23L33/105
Inventor 王薇肖庚富刘洋郭娇贾潇瑛曹浚垣张光顺王培林
Owner WUHAN INST OF VIROLOGY CHINESE ACADEMY OF SCI
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