Method for extracting adenosine triphosphate from a solution containing adenosine triphosphate
A kind of technology of adenosine triphosphate and solution, applied in the field of biomedicine
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Embodiment 1
[0071]
[0072] synthetic route:
[0073]
[0074] 1) Under the protection of Ar gas, put 2,6-diaminopyridine (2.18g, 20mmol), 2,6-dibromopyridine (14.22g, 60mmol) and potassium tert-butoxide (22.4g, 200mmol). After stirring vigorously for 0.5 h, 180 ml of dry THF was quickly added to obtain a dark green solution. Continue to react for 1.5h. Subsequently, the system was heated to 60–70°C, and CH was slowly added dropwise 3 The THF solution of I (11.36g, 80mmol CH 3 I was dissolved in 20ml THF), and the reaction was continued for about 1.5h. After the reaction, the solvent was removed by rotary evaporation, and the remaining residue was dissolved in 500ml of ethyl acetate, the organic layer was extracted with saturated brine (3*150ml), the organic layers were combined and dried over anhydrous magnesium sulfate. After column chromatography, the product 1-1 (N 2 ,N 6 -bis(6-bromopyridin-2-yl)-N 2 ,N 6 -dimethylpyridine-2,6-diamine, N 2 ,N 6 -Bis(6-bromopyridin-2-...
Embodiment 2
[0078] Utilize the heterocyclic compound that embodiment 1 obtains to extract ATP, concrete method is as follows:
[0079] (1) Dissolve ATP, ADP, AMP, MAC with heavy water respectively, obtain the first mixed solution containing ATP, ADP and AMP, wherein [ATP]=[ADP]=[AMP]=2mM, and contain ATP, ADP, The second mixed solution of AMP and MAC, wherein [ATP]=[ADP]=[AMP]=[MAC]=2mM.
[0080](2) Test the phosphorus spectrum of the first and the second mixed solution respectively, the result obtained is as follows Figure 5 and 6 As shown, among them, Figure 5 represents the phosphorus spectrum of the first mixed solution, Figure 6 Represents the phosphorus spectrum of the second mixed solution. TMP is tetramethylphosphine bromide, which is used as a quantitative internal standard, so its integral is positioned at 1.00. After integral calculation, Table 1 is obtained. Through ATP at -22.7267ppm T β The peak integration calculated that the ATP content decreased by nearly 70% befor...
Embodiment 3
[0085] Utilize the heterocyclic compound that embodiment 1 obtains to extract ATP, concrete method is as follows:
[0086] (1) Use the mixed solution of heavy water and water respectively (D 2 O:H 2 O=1:9) to dissolve ATP, ADP, AMP, MAC, obtain the first mixed solution containing ATP, ADP and AMP, wherein [ATP]=[ADP]=[AMP]=2mM, and contain ATP, ADP, The second mixed solution of AMP and MAC, wherein [ATP]=[ADP]=[AMP]=[MAC]=2mM.
[0087] (2) Test the phosphorus spectrum of the first and the second mixed solution respectively, the result obtained is as follows Figure 7 and 8 As shown, among them, Figure 7 represents the phosphorus spectrum of the first mixed solution, Figure 8 Represents the phosphorus spectrum of the second mixed solution. TMP is tetramethylphosphine bromide, which is used as a quantitative internal standard, so its integral is positioned at 1.00. After integral calculation, Table 2 is obtained. Through ATP at -22.7099ppm T β The integral of the peak ca...
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