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A kind of seedling raising method of land-sealed salmon triploid seed

A technology of Masu salmon, triploid, used in fish farming, application, climate change adaptation, etc.

Active Publication Date: 2022-03-15
北京市水生野生动植物救护中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that rainbow trout can be induced on the basis of 10-20 minutes of fertilization, 15-25 minutes of fertilization of leprosy salmon, and 25-30 minutes of fertilization of American char, so that the heat shock induction time difference among salmonids can be found Similarly, in terms of induction temperature, many studies have shown that the heat shock temperature of salmonids is between 24°C and 32°C, but some experiments have found that there is also a temperature between 26 and 26.5°C during the heat shock induction process of rainbow trout. The temperature difference of 0.5°C will also affect the final result
[0007] Therefore, in the absence of a triploid induction test report on masu salmon, the selection of heat shock induction conditions is still a major bottleneck in the field of triploid induction technology in masu salmon. Do not simply imitate the induction methods and conditions of other cold-water fish

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The seedling raising method of embodiment 1 land seal type Masu salmon triploid seed

[0037] 1. Artificial fertilization of eggs adopts dry fertilization. Avoid direct sunlight during egg collection and fertilization, dry the fish body and egg holding basin, and avoid drenching water; artificially squeeze eggs or collect eggs by laparotomy into the egg holding basin; the egg holding basin is a plastic basin with a leaking eye basin inside, with a diameter of 30cm Left and right; after the body cavity fluid is filtered out with a drain eye basin, the eggs are poured into a clean egg holding basin. The male-to-female ratio is 5:3. Collect the eggs of 5 female fish in each pot once, squeeze in the semen of 3 male fish, stir gently, add water at the same temperature in the fish pond, and rinse off the blood clots with clean water after 15-20 seconds. Egg skin and excessive semen are added to clear water and left to stand to obtain fertilized eggs; the water temperature is...

Embodiment 2

[0045] Example 2 Fertilization rate of fertilized eggs treated with heat shock at different times 15 minutes after fertilization 2h mortality rate, hatching rate, triploid rate

[0046] Fertilized eggs were 15 minutes after fertilization, and under the conditions of heat shock treatment temperature 26°C and 28°C, the 2h mortality rate of fertilized eggs in the 20min continuous treatment group was 0.14% and 1.26%, respectively, which were lower than the mortality rate (0.43% and 1.33% in the 15min continuous treatment group). %), indicating that the effect of continuous treatment time of 20min is better than that of 15min.

[0047] While at 26°C and 28°C, the hatching rate and triploid rate at 26°C of the 20min continuous treatment group were 49% and 36.7%; the hatching rate and triploid rate at 28°C were 60.92% and 53.1%, and at 28°C The overall effect of the group is better, and it can be seen that the hatching rate and triploid rate of the heat shock treatment group for 20 m...

Embodiment 3

[0048] Example 3 Fertilization rate of fertilized eggs treated with heat shock at different times 20 minutes after fertilization 2h mortality rate, hatching rate, triploid rate

[0049] Under the conditions of temperature 26°C and 28°C, at 20 minutes after fertilization, the 2h mortality rate of the 20min continuous treatment group was 1.74% and 1.58% respectively higher than that of the 15min continuous treatment group (1.21% and 0.79%), and also higher than the implementation 15 minutes after fertilization of Example 2, the treatment group (0.14% and 1.26%) under the conditions of 26°C and 28°C for 15 minutes after fertilization; under the condition of 26°C, 20 minutes after fertilization, the hatching rate and triploid rate of continuous treatment for 20 minutes were 39.4% and 33.3%; under the condition of 28 ℃, 20 minutes after fertilization, the hatching rate and triploid rate were 34.02% and 54.8% respectively after continuous treatment for 20 minutes.

[0050] At 26°C, ...

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PUM

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Abstract

The invention provides a method for raising seedlings of triploid seedlings of land-sealed masu salmon. In the present invention, the eggs and sperm of the female and male broodstock of land-sealed Masu salmon are fertilized by a dry method, and the fertilized eggs are moved into a water body at 28°C and subjected to heat shock for 15-20 minutes, and then incubated in a water body at 5-10°C for 40-60 minutes. Days, the fertilized eggs hatch into larvae. During the incubation period, the rate of fertilized eggs was as high as 93.84%, and the hatching rate of the seedlings reached more than 60.92%. The obtained larvae were detected by flow cytometry, and the triploid rate was 52.28%-78.81%. The seedling raising method of triploid seedlings of land-sealed maso salmon provided by the present invention avoids the problems of the gonad development stage of land-sealed maso salmon and the decline of fish meat quality, stagnation of growth, and increase of broodstock mortality during the spawning season. , which is obviously superior to diploid land-sealed masu salmon in terms of growth rate and disease resistance, and has better economic benefits and market value.

Description

technical field [0001] The invention relates to the technical field of aquatic organism breeding, in particular to a seedling raising method for triploid seedlings of land-sealed masu salmon. Background technique [0002] Masu salmon (Oncorhynchus masou), belonging to the family Salmonidas and the genus Salmon, is an anadromous fish that is only distributed in the waters of the Asian side of the North Pacific Ocean. It is distributed in the Tumen River, Suifen River and In the Dajia River in Taiwan. During the freshwater life period, juveniles differentiate into two ecological groups, the sea-falling type and the land-sealing type. Land-sealed populations are sexually mature in 2 years and reproduce in autumn. The average individual is 100-200g. They live in freshwater rivers all their lives and do not migrate to the sea, but participate in the reproduction of migratory populations and play an important role in the reproduction of masu salmon populations. . The domestic l...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/10A01K61/17
CPCY02A40/81
Inventor 魏凯陈春山郑伟耿琰时晓马龙
Owner 北京市水生野生动植物救护中心