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Combined drug of gossypol acetate and chemotherapeutic drug

A technology of gossypol acetate and chemotherapeutic drugs, which is applied in the field of combined drugs for the treatment of tumors, can solve the problems of chemotherapy failure and chemotherapeutic drug insensitivity, and achieve effective treatment schemes, reduce drug resistance, and achieve remarkable results

Inactive Publication Date: 2019-06-14
INST OF CHEM CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, during the course of chemotherapy, tumor cells will gradually acquire tolerance to drugs, and then become insensitive to chemotherapy drugs, which is the main reason for clinical chemotherapy failure

Method used

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  • Combined drug of gossypol acetate and chemotherapeutic drug
  • Combined drug of gossypol acetate and chemotherapeutic drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 2

[0040] Example 2 GAA combined with cisplatin enhances anti-tumor effect

[0041] In this example, the combination of GAA and cisplatin was used in the lung adenocarcinoma cell line A549, and the sensitization effect of GAA on the curative effect of cisplatin in the LRPPRC-positive lung adenocarcinoma cell line A549 was evaluated.

[0042] The lung adenocarcinoma cell line A549 in the logarithmic growth phase was digested and counted by trypsin, seeded in a 96-well plate of the xCELLigence RTCA-MP system at a density of 2000 cells / well, cultured for 24 hours and started to add drugs after adhering to the wall. Four groups were set up, which were the combination group with GAA (5uM), cisplatin (5uM) or GAA (5uM)+cisplatin (5uM), respectively, and the group with only PBS buffer as the blank control group. The cell number data information of each well was collected every 30 minutes, and the detection continued for 72 hours. According to the collected cell number information, the ...

Embodiment 3

[0044] Example 3 GAA has a synergistic effect in combination with chemotherapeutic drugs

[0045] Using the standard synergy index calculation formula Q=E(A+B) / (E(A)+E(B)-E(A)×E(B)), (E(A+B) is the inhibitory effect of the combination of two drugs rate, E(A) is the inhibition rate of drug A alone, and E(A) is the inhibition rate of drug B alone) to carry out statistics on the synergistic effect of GAA and cisplatin, when the Q value is greater than 1.15, it is a synergistic effect. In the lung adenocarcinoma cell line A549, the inhibition rates of GAA (5 μM), cisplatin (5 μM) and the combination of the two treated cells for 72 hours were: E(GAA)=0.28, E(cisplatin)=0.51, E(cisplatin)=0.51, E (GAA+cisplatin)=0.81, Q=1.23, showing a synergistic effect.

[0046] It should be noted that Examples 1-3 take lung adenocarcinoma cells as an example for verification. Of course, cells such as breast cancer, colon cancer, rectal cancer, esophageal cancer, lung squamous cell carcinoma, pan...

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Abstract

The invention relates to a combined drug for treating tumors, in particular to a combined drug of gossypol acetate and a chemotherapeutic drug. The combined drug has a good synergistic interaction effect, and has a stronger effect of inhibiting tumors than a single drug; moreover, the combined drug has the effect of chemosensitization, and can effectively reduce the drug resistance of tumor cellsto the chemotherapeutic drug. According to the combined drug, a new and more effective treatment scheme is provided for clinical treatment of tumor diseases, and the clinical application prospect is good.

Description

technical field [0001] The invention relates to a combined drug for treating tumors, in particular to a combined drug of gossypol acetate (GAA) and chemotherapy drugs. Background technique [0002] Cancer is a major disease that causes human death, and the number of deaths due to cancer is about 7 million worldwide every year. Due to the lack of early cancer diagnosis and screening technology at this stage, a large number of cancer patients are already in the advanced stage of tumor when they first see a doctor, and they have lost the chance of surgical resection and cure. Chemotherapy and radiotherapy are still the main treatment methods for advanced tumors at this stage. However, during the course of chemotherapy, tumor cells will gradually acquire tolerance to drugs, and then become insensitive to chemotherapy drugs, which is also the main reason for clinical chemotherapy failure. [0003] BCL2 (B-cell lymphoma-2) protein family plays a decisive role in the anti-apoptot...

Claims

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Application Information

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IPC IPC(8): A61K45/00A61K31/11A61K45/06A61P35/00
CPCA61K31/11A61K45/00A61K45/06A61P35/00
Inventor 方晓红周卫孙国贵徐丽
Owner INST OF CHEM CHINESE ACAD OF SCI
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