Method for extracting flavonoids from pteridium aquilinum

An extraction method and bracken technology are applied in the field of extraction of flavonoids in bracken, can solve the problems of less research on flavonoids antioxidant properties and the like, and achieve the effects of improving antioxidant activity and improving extraction rate

Pending Publication Date: 2019-06-14
HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Flavonoids themselves have certain antioxidant properties, but there are few studies on the antioxidant properties of flavonoids in bracken

Method used

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  • Method for extracting flavonoids from pteridium aquilinum
  • Method for extracting flavonoids from pteridium aquilinum

Examples

Experimental program
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Effect test

Embodiment 1

[0023] A method for extracting flavonoids from bracken of the present invention, its specific operation method is as follows:

[0024] a. Pretreatment: Wash the bracken, dry it at 80°C, crush it to 100 mesh, and make bracken powder for use;

[0025] b. Protein removal: mix the bracken powder prepared in step a with water, the mass ratio of water to bracken powder is 1:5, add alkaline protease at 50°C and pH8.0, alkaline protease and The mass ratio of the bracken powder mixture is 1:30, and after stirring at a constant temperature for 30 minutes, the enzyme is extinguished at 95°C for 5 minutes to obtain the enzymatic solution Ⅰ;

[0026] c. Defiber: Add cellulase to the enzymatic hydrolysis solution I prepared in step b at 40°C and pH 4.0, the mass ratio of cellulase and bracken powder mixture is 1:30, and stir at constant temperature After 30 minutes, inactivate the enzyme at 95°C for 5 minutes to obtain enzymatic solution II;

[0027] d. Extraction: mix the enzymolysis sol...

Embodiment 2

[0031] A method for extracting flavonoids from bracken of the present invention, its specific operation method is as follows:

[0032] a. Pretreatment: wash the bracken, dry it at 85°C, crush it to 100 mesh, and make bracken powder for use;

[0033] b. Protein removal: mix the bracken powder prepared in step a with water, the mass ratio of water and bracken powder is 1:15, add alkaline protease at 60°C and pH8.5, alkaline protease and The mass ratio of the bracken powder mixture is 1:50, and after stirring at a constant temperature for 60 minutes, the enzyme is inactivated at 95°C for 5 minutes to obtain the enzymatic solution Ⅰ;

[0034] c. Defiber: add cellulase to the enzymolysis solution I prepared in step b at 60°C and pH 5.5, the mass ratio of cellulase to bracken powder mixture is 1:50, and stir at constant temperature After 60 minutes, inactivate the enzyme at 95°C for 5 minutes to obtain the enzymatic solution II;

[0035] d. Extraction: Mix the enzymatic hydrolysis...

Embodiment 3

[0039] A method for extracting flavonoids from bracken of the present invention, its specific operation method is as follows:

[0040] a. Pretreatment: Wash the bracken, dry it at 80°C, crush it to 100 mesh, and make bracken powder for use;

[0041] b. Protein removal: mix the bracken powder prepared in step a with water, the mass ratio of water to bracken powder is 1:7, add alkaline protease at 55°C and pH8.3, and alkaline protease and The mass ratio of the bracken powder mixture is 1:35, and after stirring at a constant temperature for 40 minutes, the enzyme is extinguished at 95°C for 5 minutes to obtain the enzymatic solution Ⅰ;

[0042] c. Defiber: add cellulase to the enzymolysis solution I prepared in step b at 50°C and pH 5.0, the mass ratio of cellulase to bracken powder mixture is 1:35, and stir at constant temperature After 45 minutes, inactivate the enzyme at 95°C for 5 minutes to obtain enzymatic solution II;

[0043] d. Leaching: Mix the enzymolysis solution II...

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Abstract

The invention discloses a method for extracting flavonoids from pteridium aquilinum, and relates to the technical field of flavone extraction methods, and the method specifically comprises six steps of pretreatment, protein removal, fiber removal, extraction, concentration and freeze-drying. According to the method disclosed by the invention, the flavonoids is extracted from the pteridium aquilinum, the yield of the flavonoids is up to 9.0%, protein and cellulose are sequentially removed by using an enzymolysis method, on one hand, the influence of impurities on extraction is removed, and on the other hand, the redundant structure can be opened, and the extraction rate of the flavonoids is better improved. Meanwhile, the protein and the cellulose can be removed, so that the antioxidant activity of the product can be improved; meanwhile, the antioxidant activity difference of the finished product and VC is not large, and the product can be used as an antioxidant for food.

Description

Technical field: [0001] The invention relates to the technical field of extraction methods of flavonoids, in particular to an extraction method of flavonoids in bracken. Background technique: [0002] Bracken is a wild vegetable that grows widely in sparse coniferous and broadleaf mixed forests, and is eaten as dried vegetables and cans. Studies have found that bracken is rich in flavonoids, so bracken can be further processed to increase its added value. Flavonoids themselves have certain antioxidant properties, but there are few studies on the antioxidant properties of flavonoids in bracken. At the same time, different extraction methods have different antioxidant effects of flavonoids. Therefore, it is necessary to study the extraction methods of flavonoids in bracken for the antioxidant effects of flavonoids. Invention content: [0003] The purpose of the present invention is to provide a method for extracting flavonoids in bracken in order to overcome the shortcomin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/30C07D311/40A23L3/3544
Inventor 吴海涛
Owner HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY
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