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Method for producing microbial fertilizer from coal gangue

A technology of microbial fertilizer and coal gangue, which is applied in fertilizer mixture, fertilization device, application and other directions, can solve the problem that plants cannot be directly absorbed and utilized, and achieves the effects of low cost, simple and reliable process, and environmental friendliness.

Active Publication Date: 2019-06-28
GUIZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The phosphorus and potassium contained in it mostly exist in the form of insoluble minerals, which cannot be directly absorbed and utilized by plants.

Method used

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  • Method for producing microbial fertilizer from coal gangue
  • Method for producing microbial fertilizer from coal gangue
  • Method for producing microbial fertilizer from coal gangue

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0023] 1. See the sequence listing for the sequence of GZU-Ch01, and the drawing of the growth curve of the strain of GZU-Ch01

[0024] 1) Preparation of seed solution. The strain was inoculated in LB liquid medium with an inoculation loop, and cultivated to the logarithmic phase at 30°C.

[0025] 2) Vaccination. Take 10 mL of the above seed solution and inoculate it into 200 mL of liquid LB medium, mix well and take 5 mL of the mixed solution and put them in 18 marked sterile test tubes.

[0026] 3) Cultivate. Place the inoculated test tubes at 30°C and incubate in a shaker at 170r / min, and take them out after cultivating for the corresponding time.

[0027] 4) Measure. Use uninoculated LB liquid culture medium as a blank, and conduct photoelectric turbidimetry together with the sample to be tested at a wavelength of 600nm.

[0028] Measurement results such as figure 1 , as can be seen from the figure, 0-10h is the lag phase, 10-15h is the logarithmic phase, 15h-25h is ...

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Abstract

The invention discloses a method for producing microbial fertilizer from coal gangue. The method comprises the following steps: with the coal gangue with a basic raw material, doping low-grade phosphorite and potassium mineral, and carrying out dissociation by virtue of golden bacillus GZU-Ch01, so as to prepare the microbial fertilizer. By dissociating the coal gangue by virtue of the golden bacillus GZU-Ch01, useful nutrients in the coal gangue are released, and the preparation of the coal gangue microbial fertilizer is a novel way for the utilization of the coal gangue. The preparation process is simple, and mild in conditions, and is environment-friendly use scheme of the coal gangue.

Description

technical field [0001] The invention relates to a method for producing microbial fertilizer using coal gangue as raw material, in particular to a method for producing microbial fertilizer by dissociating coal gangue with Chryseobacterium strain GZU-Ch01. Background technique [0002] my country's energy structure is dominated by coal resources, and a large amount of coal is consumed every year. Coal gangue is a solid waste with low carbon content produced in the process of coal mining and washing, accounting for about 15%-20% of the original coal output. Abandoned coal gangue is mostly piled up in the open air, forming countless coal gangue hills. Over the years of production accumulation, it is estimated that billions of tons of coal gangue are discarded in various coal-producing areas in China. Coal gangue stacked in the open air will gradually weather after being washed by rainwater, and the harmful substances in it will be gradually dissolved, and will flow into the so...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/04C05G3/80
Inventor 谢承卫高弦钟艳王应兰张景宁
Owner GUIZHOU UNIV
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