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Preparation and application of a compound microbial agent with disease-resistant and growth-promoting functions

A compound microbial agent and growth-promoting technology, which is applied in the field of fertilizers and can solve problems such as poor application effects

Active Publication Date: 2021-08-17
MICROBIAL FERMENTATION ENG RES CENT CO LTD OF YUNNAN PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Existing bacterial agents generally use a single strain. In actual use, the application effect is not good, and it cannot be widely promoted.

Method used

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  • Preparation and application of a compound microbial agent with disease-resistant and growth-promoting functions
  • Preparation and application of a compound microbial agent with disease-resistant and growth-promoting functions
  • Preparation and application of a compound microbial agent with disease-resistant and growth-promoting functions

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Experimental program
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Effect test

preparation example Construction

[0022] A preparation method of a compound microbial bacterial agent with disease-resistant and growth-promoting functions, comprising the following steps:

[0023] (1) select the bacillus subtilis that numbering is WSWFJ-2, the bacillus subtilis that numbering is WSWFJ-7, the bacillus amyloliquefaciens that numbering is WSWFJ-8, the bacillus amyloliquefaciens that numbering is WSWFJ-11, carry out separate fermentation respectively After the fermentation is completed, the number of effective viable bacteria in the fermentation liquid of each strain should be greater than 2.0×10 9 cfu / ml;

[0024] (2) 0~35 parts of fermentation liquid of Bacillus subtilis strain numbered WSWFJ-2, 0~35 parts of fermentation liquid of Bacillus subtilis strain numbered WSWFJ-7, amyloliquefaciens spores numbered WSWFJ-8 by weight percentage Mix 25-50 parts of Bacillus strain fermentation liquid with 25-50 parts of Bacillus amyloliquefaciens strain fermentation liquid numbered WSWFJ-11 to obtain a c...

Embodiment 1

[0035] A compound microbial bacterial agent with disease-resistance and growth-promoting function, which consists of Bacillus subtilis numbered WSWFJ-2, Bacillus subtilis numbered WSWFJ-7, and solution numbered WSWFJ-8 Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) and Bacillus amyloliquefaciens (Bacillus amyloliquefaciens) numbered WSWFJ-11 were assembled.

[0036] The preparation method of a compound microbial bacterial agent with the function of disease resistance and growth promotion is as follows: each bacterial strain is fermented separately, and after the fermentation is completed, the effective number of viable bacteria in the fermentation liquid of each bacterial strain should reach 2.0×10 9 More than cfu / ml, mix 25 parts of WSWFJ-2 strain fermentation broth, 25 parts of WSWFJ-7 strain fermentation broth, 25 parts of WSWFJ-8 strain fermentation broth, and 25 parts of WSWFJ-11 strain fermentation broth by weight percentage , to obtain a compound microbial bact...

Embodiment 2

[0045] A compound microbial agent with the function of disease resistance and growth promotion, composed of two strains of Bacillus amyloliquefaciens numbered WSWFJ-8 and Bacillus amyloliquefaciens numbered WSWFJ-11 .

[0046] The preparation method of a compound microbial bacterial agent with the function of disease resistance and growth promotion is as follows: each bacterial strain is fermented separately, and after the fermentation is completed, the effective number of viable bacteria in the fermentation liquid of each bacterial strain should reach 2.0×10 9 For more than cfu / ml, mix 50 parts of WSWFJ-8 strain fermentation liquid and 50 parts of WSWFJ-11 strain fermentation liquid according to weight percentage to obtain a compound microbial bacterial agent with disease-resistant and growth-promoting functions. The compound microbial bacterial agent of this formula is suitable for the planting of crops such as oilseeds, grains, and fruit trees.

[0047] The application of ...

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PUM

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Abstract

The invention discloses the preparation and application of a compound microbial agent with the function of disease resistance and growth promotion, and the preparation method comprises the following steps: (1) selecting Bacillus subtilis numbered WSWFJ‑2 and Bacillus subtilis numbered WSWFJ‑7 Bacillus, numbering is the bacillus amyloliquefaciens of WSWFJ‑8, numbering is the bacillus amyloliquefaciens of WSWFJ‑11, ferments respectively; 35 parts, numbering 0-35 parts of Bacillus subtilis strain fermentation liquid numbered WSWFJ-7, 25-50 parts of Bacillus amyloliquefaciens strain fermentation liquid numbered WSWFJ-8, fermentation of Bacillus amyloliquefaciens strain numbered WSWFJ-11 Mix 25 to 50 parts of the solution to obtain a compound microbial agent with disease resistance and growth-promoting functions; the compound microbial agent prepared by the preparation method is used in agricultural production, which can effectively reduce the use of chemical pesticides and promote green agriculture. development of.

Description

technical field [0001] The invention relates to the field of fertilizers, in particular to the preparation and application of a compound microbial agent with the function of disease resistance and growth promotion. Background technique [0002] Microbial agent refers to the live bacterial preparation made by processing the fermentation broth of the target microorganisms (effective bacteria) by using porous substances as adsorbents (such as peat and vermiculite) after industrial production and expansion. This bacterial agent is used for seed dressing or root dipping, and has the functions of directly or indirectly improving soil, restoring soil fertility, preventing soil-borne diseases, maintaining the balance of rhizosphere microbial flora, and degrading toxic substances. Proper use of agricultural microbial agents can increase the yield of agricultural products, improve the quality of agricultural products, reduce the amount of chemical fertilizers, reduce costs, improve so...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P21/00C12R1/125C12R1/07
CPCC12N1/20A01N63/10
Inventor 张琪尚海丽崔文艳王斌何月秋赵崇钧赵学通
Owner MICROBIAL FERMENTATION ENG RES CENT CO LTD OF YUNNAN PROVINCE