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A technology for microfluidic channels and liquids, applied in fluid controllers, chemical instruments and methods, biochemical equipment and methods, etc.
Inactive Publication Date: 2019-07-05
ARCHERDX
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Abstract
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These methods often include multiple enzymatic, purification, and preparative steps that make the method laborious and error-prone, including errors associated with contamination, systematic user error, and processing bias
Therefore, it is often difficult to perform such processes reliably and reproducibly, especially when these processes are performed commercially, such as in multiplex or high-throughput environments.
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example 1
[0267] The following example illustrates the operation of the channel system such that a sample fluid is transferred to a container of the channel system.
[0268] exist Figure 25 to Figure 26 An exemplary channel system 1700 is shown in .
[0269] 1) Valve 1710 is actuated such that first common microfluidic channel 1750 is in fluid communication with second common microfluidic channel 1760 .
[0270] 2) Valve 1720 is actuated such that second common microfluidic channel 1760 is in fluid communication with syringe pump 1780 .
[0271] 3) Optionally, valve 1730 is actuated such that first common microfluidic channel 1750 is in fluid communication with inlet channel 1770 .
[0272] 4) Optionally, if step 3 is performed, suction is performed with a syringe pump 1780 so that air is sucked into the first common microfluidic channel 1750 .
[0273] 5) Actuate the first valve 1730 such that the first common microfluidic channel 1750 is in fluid communication with the sample well...
example 2
[0278] The following example illustrates the operation of the channel system such that bulk fluid is transferred to the container of the channel system.
[0279] exist Figure 25 to Figure 26 An exemplary channel system 1700 is shown in .
[0280] 1) Valve 1710 is actuated such that second common microfluidic channel 1760 is in fluid communication with microfluidic channel 1742 .
[0281] 2) Valve 1740 is actuated so that microfluidic channel 1742 and bulk fluid container 1745 are in fluid communication.
[0282] 3) Suction with syringe pump 1780 such that bulk fluid from bulk fluid container 1745 is drawn into second common microfluidic channel 1760 .
[0283] 4) Actuate the valve 1710 such that the first common microfluidic channel 1750 is in fluid communication with the second microfluidic channel 1760 .
[0284] 5) Actuate valve 1730 such that first common microfluidic channel 1750 is in fluid communication with waste channel 1775 .
[0285] 6) Suction with syringe pum...
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Abstract
Fluidic systems including cartridges with modular components (cassettes) and / or microfluidic channels for performing chemical and / or biological analyses are provided. The systems described herein include a cartridge comprising, in some embodiments, a frame, one or more cassettes which may be inserted into the frame, and a channel system for transporting fluids. In certain embodiments, the one or more cassettes comprise one or more reservoirs or vessels configured to contain and / or receive a fluid (e.g., a stored reagent, a sample). In some cases, the stored reagent may include one or more lyospheres. The systems and methods described herein may be useful for performing chemical and / or biological reactions including polymerase chain reactions (PCR) such as those performed within a laboratory, clinical (e.g., hospital), or research setting.
Description
[0001] related application [0002] This application is claimed under 35 U.S.C. §119(e) U.S. Provisional Patent Application Nos. 62 / 398,841, 62 / 399,152, 62 / 399,157, 62 / 399,184, 62 / 399,195, 62 / 399,205 No. 62 / 399,211 and 62 / 399,219, each of which was filed on September 23, 2016 and filed in 2017 pursuant to 35 U.S.C. §§ 120 and 365(c) Priority of PCT International Application No. PCT / US2017 / 051924, filed September 15, and priority of PCT International Application No. PCT / US2017 / 051927, filed September 15, 2017, of PCT International Application No. PCT / US2017 / 051924 claims priority under 35U.S.C. §119(e) to U.S. Provisional Patent Application No. 62 / 395,339, filed September 15, 2016, and PCT International Application No. PCT / US2017 / 051927 under 35U. S.C. §119(e) claims priority to U.S. Provisional Patent Application No. 62 / 395,347, filed September 15, 2016, the entire contents of each of which are incorporated herein by reference. technical field [0003] The present invention...
Claims
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