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Method for evaluating risk exposure on animal body by tobacco-specific nitrosamines

An exposure risk, animal body technology, applied in the field of exposure risk assessment of carcinogens, can solve problems such as the inability to quantify the potential exposure risk of TSNAs

Active Publication Date: 2019-07-09
ZHENGZHOU TOBACCO RES INST OF CNTC
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for evaluating the exposure risk of tobacco-specific nitrosamines to animal bodies, so as to solve the problem that the existing methods cannot quantitatively evaluate the potential exposure risk of TSNAs

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  • Method for evaluating risk exposure on animal body by tobacco-specific nitrosamines
  • Method for evaluating risk exposure on animal body by tobacco-specific nitrosamines
  • Method for evaluating risk exposure on animal body by tobacco-specific nitrosamines

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Embodiment Construction

[0032] The present invention mainly uses a certain dose of representative TSNAs (NNK, NNAL, NNN, NAB) solution to expose animal models, and uses high performance liquid chromatography-tandem mass spectrometry to measure the direct metabolites of TSNAs in blood and the indirect metabolites in liver tissue , on the basis of obtaining the relative amount of TSNAs and their metabolites entering the systemic circulation, according to the ratio of the total amount of α-hydroxylation metabolic pathway products in the blood to the relative amount of TSNAs entering the systemic circulation (α-hydroxylation coefficient) and the main metabolic organ The production of representative DNA adducts within the body was used to assess the degree of α-hydroxylation in different individuals after exposure to TSNAs.

[0033] On the one hand, this method uses the ratio of direct metabolites in the blood to reflect the average degree of α-hydroxylation metabolism of TSNAs in the body; The degree of ...

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Abstract

The invention relates to a method for evaluating risk exposure on an animal body by tobacco-specific nitrosamines. The method comprises the following steps: 1) detecting content of TSNAs and metabolite thereof; computing an AUC value, computing an alpha-hydroxylation coefficient according to the AUC value, and detecting content of the O6-mG and O2-POB-dT; and acquiring the yield of the representative DNA adduct according to the sum of the content values of the O6-mG and O2-POB-dT; 2) evaluating the alpha-hydroxylation metabolic degree according to the alpha-hydroxylation coefficient and the representative DNA adduct yield, and predicting the exposure risk on the animal body by the TSNAs. Through the method disclosed by the invention, the alpha-hydroxylation reaction degree in different individuals after the TSNAs is exposed can be evaluated according to the alpha-hydroxylation coefficient and the yield of the representative DNA adduct, thereby providing quantitative index for the potential exposure risk of the TSNAs.

Description

technical field [0001] The invention belongs to the field of exposure risk assessment of carcinogens, and in particular relates to a method for assessing the exposure risk of tobacco-specific nitrosamines (TSNAs) to animal bodies. Background technique [0002] TSNAs are a unique group of N-nitrosamine harmful components in tobacco, which are formed by nitrosation of tobacco alkaloids in the process of tobacco preparation and fermentation, and have always been a hot spot in the research of smoking and health issues. There are eight known TSNAs, NNK, NNN, NAB, NAT, NNAL, iso-NNAL, NNA, and iso-NNAC. NAB and NAT are directly formed by the nitrosation of anatabine and anatabine, and NNN is mainly formed by the nitrosation of nornicotine, while the nitrosation of nicotine mainly forms NNK and is further converted into a small amount of NNAL, and a very small part Nicotine nitrosation can also form NNA and be further converted into iso-NNAL or iso-NNAC. At present, it is believe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8813
Inventor 张建勋毛健刘俊辉孟庆华冯明飞赵贝贝郎海磊张启东卢斌斌王磊刘珊范武史清照
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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