Suspension mixed cultivation method of Utricularia aurea and Lemna minor
A technology of cucurbita japonica and duckweed is applied in the field of water pollution control, which can solve the problems of non-floating, poor growth, and photosynthesis effects of submerged plants, and achieve the effect of ensuring sustainable utilization and promoting growth.
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Embodiment 1
[0037] This embodiment provides a method for mass and rapid propagation of the endangered species of U. chrysalis, which can effectively promote the sustainable development and utilization of U. chrysalis resources, which includes the following steps:
[0038] Step 1: Disinfect and sterilize the explants; select healthy and strong branches and leaves of Uriella chrysalis as explants, and then disinfect and sterilize the explants.
[0039] Step 2: induction culture; in a sterile environment, inoculate the sterilized explants into sterile MS solid medium for induction culture, and cultivate until the young shoots grow to 1.0-2.0 cm.
[0040] Step 3: Proliferation culture; in a sterile environment, transfer the sprouts to MS liquid culture for multiplication culture, and proliferate and culture until the sterile seedlings grow to 6-10 cm.
[0041] Step 4: seedling hardening and transplanting; the aseptic seedlings are hardened, and transplanted after 1-3 days of hardening.
Embodiment 2
[0043] This embodiment provides a method for mass and rapid propagation of the endangered species of U. chrysalis, which can effectively promote the sustainable development and utilization of U. chrysalis resources, which includes the following steps:
[0044]Step 1: Disinfect and sterilize the explants; select healthy and strong branches and leaves of Uriella chrysalis as explants, first soak them in detergent, wash them with tap water and sterile water in turn, drain them with a volume fraction of 75% Soak in alcohol for 0.5-1min, wash with sterile water, then disinfect with 0.1% mercuric chloride for 5-10min, wash with sterile water, and drain the water.
[0045] Step 2: induction culture; in a sterile environment, inoculate the sterilized explants into sterile MS solid medium to induce culture, at a temperature of 25-28°C and a light intensity of 1800-2000Lux Next, cultivate until the young shoots grow to 1.0-2.0cm. 0.8-1.5 mg / L of 6-benzylpurine, 0.5-1.0 mg / L of naphthal...
Embodiment 3
[0049] This embodiment provides a suspension polyculture method of U. chrysalis and duckweed. In the method, U. chrysalis and duckweed are polycultured, and the two are suspended on the surface of the water body for culture through a mesh culture box with an open top.
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