Application of Bacteroides fragilis extract in preparation of composition for enhancing immunity
A Bacteroides fragilis, immunity-enhancing technology, applied in the field of microorganisms
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Embodiment 1
[0062] Example 1 Preparation of Bacteroides fragilis Extract
[0063] 1. Fermentation culture of Bacteroides fragilis
[0064] Streak inoculation of strains on blood plate, anaerobic culture for 48h. Observe the colony morphological characteristics, staining characteristics, size, club shape and distribution, etc.
[0065] Colony characteristics: Bacteroides fragilis ZY-312 is cultured on a blood plate for 48 hours, showing circular slightly convex, translucent, white, smooth surface, non-hemolytic, colony diameter between 1-3mm, see figure 1 .
[0066] Morphology under the microscope: Gram-stained Bacteroides fragilis ZY-312 is a Gram-negative bacterium with a typical rod shape, blunt rounded ends and dense staining, and the uncolored part in the middle of the bacteria is like a vacuole, see figure 2.
[0067] Select a single colony and inoculate it in tryptone broth for fermentation and culture for 8 hours (at a temperature of 37°C). The obtained bacterial solution is c...
Embodiment 2
[0074] Example 2 Effect of Bacteroides fragilis Extract on Carbon Clearance Experiment in Mice
[0075] 1. Experimental animals and grouping
[0076] In this example, 50 female mice of SPF grade Kunming species were used, and each experimental mouse was assigned a unique number. Prior to grouping animals, the item number, species / strain, sex, cage number, and animal number should be marked on the cage label. Using BioBook software, the mice were randomly divided into 5 groups according to their initial body weight, namely blank control group, positive control group, low-, medium-, and high-dose groups of B. fragilis extract, with 10 mice in each group.
[0077] 2. Dose selection and test substance administration
[0078] In this example, according to the recommended oral dose of mice, the doses of the Bacteroides fragilis extract of the present invention in the low, medium and high dose groups are respectively 1.25 mg / kg.Bw, 2.5 mg / kg.Bw, 5 mg / kg .Bw. Bacteroides fragilis ...
Embodiment 3
[0083] Example 3 Effect of Bacteroides fragilis Extract on Delayed Hypersensitivity
[0084] 1. Experimental animals and grouping
[0085] With reference to the experimental animals and groupings of Example 2.
[0086] 2. Dose selection and test substance administration
[0087] Refer to Example 2 for dosage selection and test substance administration.
[0088] 3. Delayed type hypersensitivity (DTH)
[0089] On the day when the administration was completed, the abdominal skin of each mouse was depilated with barium sulfide, with a range of about 3cm*3cm, and then sensitized with 50 μL of freshly prepared 2% DNCB solution, and 5 days later, 10 μL of 1% DNCB solution was evenly applied to the mice Attack behind the right ear. 24 hours after the challenge, after the mice were anesthetized with 30 mg / kg of Shutai, the left and right ears with a diameter of 5 mm were taken from each mouse, and the thickness was measured and weighed. The swelling degree of the ears (the differen...
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