Method for extracting and separating flavonoid substances from soil
A technology for separating flavonoids and soil, applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve the problems of extraction and interference of difficult flavonoids
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[0042] Embodiment 1 A method of extracting and separating flavonoids from soil
[0043] Including the following steps:
[0044] (1) Air-dry the blank soil sample and pass it through a 20-mesh sieve; weigh 5 g of soil into a centrifuge bottle, add 100 μL of mixed standard solution, vortex and oscillate to mix, and let stand for 30 minutes;
[0045] (2) Add the extract solution to the soil sample, vortex oscillate and mix well, then ultrasonically extract for 30 minutes, centrifuge at 5000rpm for 5 minutes, and collect the supernatant; repeat this step for the precipitate after centrifugation, and combine the supernatant; The supernatant was concentrated using a rotary evaporator to remove methanol.
[0046] (3) Activate the SPE small column, and pass all the liquid obtained by evaporation and concentration through the activated SPE small column; then wash the column with 20 mL of methanol, and collect the washing liquid.
[0047] Activation operation: After fixing the SPE car...
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