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Method for extracting and separating flavonoid substances from soil

A technology for separating flavonoids and soil, applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc., can solve the problems of extraction and interference of difficult flavonoids

Active Publication Date: 2019-07-19
INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI
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Problems solved by technology

[0004] However, in addition to astragalin, the soil also contains flavonoids such as kaempferol, quercetin, and myricetin. Due to the different properties of each substance and the interference of organic and inorganic substances in the soil, it is difficult to use the above extraction method. At the same time, other flavonoids in the soil are extracted

Method used

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  • Method for extracting and separating flavonoid substances from soil
  • Method for extracting and separating flavonoid substances from soil
  • Method for extracting and separating flavonoid substances from soil

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Embodiment 1

[0042] Embodiment 1 A method of extracting and separating flavonoids from soil

[0043] Including the following steps:

[0044] (1) Air-dry the blank soil sample and pass it through a 20-mesh sieve; weigh 5 g of soil into a centrifuge bottle, add 100 μL of mixed standard solution, vortex and oscillate to mix, and let stand for 30 minutes;

[0045] (2) Add the extract solution to the soil sample, vortex oscillate and mix well, then ultrasonically extract for 30 minutes, centrifuge at 5000rpm for 5 minutes, and collect the supernatant; repeat this step for the precipitate after centrifugation, and combine the supernatant; The supernatant was concentrated using a rotary evaporator to remove methanol.

[0046] (3) Activate the SPE small column, and pass all the liquid obtained by evaporation and concentration through the activated SPE small column; then wash the column with 20 mL of methanol, and collect the washing liquid.

[0047] Activation operation: After fixing the SPE car...

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Abstract

The invention relates to a method for extracting and separating flavonoid substances from soil. The method comprises the steps of (1) drying soil samples, and performing screening; (2) adding an extracting solution, performing uniform mixing, performing ultrasonic extraction, performing centrifuging, collecting supernatant, repeatedly operating the steps on centrifuged precipitate, combining supernatant, and performing evaporation to remove methanol, wherein the extracting solution consists of EDTA-2Na and methanol, and the mass ratio of the EDTA-2Na to the soil samples is (0.028-0.32) to 1; and (3) performing purification so as to obtain the flavonoid substances. The inventor finds that when the EDTA-2Na and the methanol are added to soil in a specific proportion, various flavonoid substances can be sufficiently extracted in a short time, and remarkable technical effects can be obtained.

Description

technical field [0001] The invention relates to a method for extracting and separating flavonoids from soil, and belongs to the technical field of extraction and separation. Background technique [0002] Flavonoids produced by plants (shivinin, kaempferol, quercetin, myricetin, etc.) are an important class of secondary metabolites, and their synthesis and accumulation in plants are closely related to the growth environment of plants; Moreover, flavonoids also participate in the stress resistance process of plants, and are an important protection mechanism for plants to resist external adverse environments, and occupy an irreplaceable position in the entire metabolism of plants. In addition, after the flavonoids are secreted into the soil by the plant root system, they play an important role in the plant and the rhizosphere environment of the plant. Therefore, it is very important to study the environmental behavior of flavonoids in soil, and the premise of studying this beh...

Claims

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Application Information

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IPC IPC(8): C07H1/08C07H17/07C07D311/40C07D311/30
CPCC07D311/30C07D311/40C07H1/08C07H17/07
Inventor 付卫东张瑞海张国良宋振孙宝利王忠辉
Owner INST OF ENVIRONMENT & SUSTAINABLE DEV IN AGRI CHINESE ACADEMY OF AGRI SCI
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