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Flow cytometer based on micro-fluidic three-dimensional focusing technology

A flow cytometer and microfluidic technology, applied in the field of flow cytometry, can solve the problems of cell adhesion and transcendence, and achieve the effect of high resolution, short production cycle and clear details

Active Publication Date: 2019-07-23
XIAN UNIV OF TECH
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0003] The purpose of the present invention is to provide a flow cytometer based on microfluidic three-dimensional focusing technology, which solves the problems of cell adhesion and beyond flow in the existing flow cytometer using trident microfluidic chips

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  • Flow cytometer based on micro-fluidic three-dimensional focusing technology
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  • Flow cytometer based on micro-fluidic three-dimensional focusing technology

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Embodiment Construction

[0020] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0021] The present invention is based on the flow cytometer of microfluidic three-dimensional focusing technology, such as figure 1 As shown, it includes a microfluidic chip 1, a parallel light source 2 is arranged directly above the microfluidic chip 1, a lensless sensor 3 is arranged on the lower surface of the microfluidic chip 1, and a data processing module 4 is connected to the lensless sensor 3.

[0022] The data processing module 4 has three structures, which are as follows:

[0023] In the first structure, an ASIC chip is arranged in the data processing module 4;

[0024] In the second structure, an embedded system is set in the data processing module 4;

[0025] In the third structure, an FPGA system is set in the data processing module 4 .

[0026] Such as figure 2 As shown, the microfluidic chip 1 is provided with a microchan...

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Abstract

The invention discloses a flow cytometer based on a micro-fluidic three-dimensional focusing technology. The flow cytometer comprises a micro-fluidic chip; a parallel light source is arranged over themicro-fluidic chip, and a lensless sensor is arranged on the lower surface of the micro-fluidic chip; and the lensless sensor is connected with a data processing module. Three-dimensional focusing ofcells is achieved, and thus a cell image is convenient to collect; and in a microchannel of a main fluidic chip, because of blocking of a triangular obstructing tiled plate, flow clamping focusing liquid is formed in a focusing channel, thus cell sap is focused for the first time, sample buffer solutions on the two sides of an outlet of the focusing channel flow out from a sheath fluid channel tofocus the cell sap flowing out from the focusing channel again, thus the cells in the cell sap evenly flow through a lensless sensor image collecting area in single rows, lensless imaging and algorithm processing are facilitated, a collected cell image is clear in detail and high in resolution ratio, and the problems of cell adhesion and transcending flow are avoided.

Description

technical field [0001] The invention belongs to the technical field of cell detection devices, and relates to a flow cytometer based on microfluidic three-dimensional focusing technology. Background technique [0002] In recent years, emerging microfluidic chips have the characteristics of short production cycle, low cost, high efficiency and reliable structure, and have been widely used in biological cell analysis and medical fields. At present, most of the microfluidic devices are based on the principle of fluid mechanics, and micro-pump controllers are used for micro-injection to achieve micro-fluidic focusing. Among them, the microfluidic chip with a trident structure is the most widely used. The basic method is to pass the cell dilution buffer at both ends of the cell liquid, and pass the sample cell liquid in the middle. This is beneficial to protect the cell fluid and eliminate the interference of other non-detection particle movement. This structure enables the cell...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N15/14B01L3/00
CPCB01L3/5027G01N15/1434G01N2015/1445G01N2015/1452
Inventor 戴力李帅军李建伟余宁梅
Owner XIAN UNIV OF TECH
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