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Method for agglutinating erythrocytes, method for separating erythrocytes, and hemagglutination reagent

A technology for erythrocyte agglutination and erythrocytes, which is applied in biological testing, material analysis, sampling, etc., and can solve the problems of unreported bile acid surfactants, etc.

Active Publication Date: 2019-07-30
DENKA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there have been no reported examples of using bile acid-based surfactants when agglutinating erythrocytes

Method used

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  • Method for agglutinating erythrocytes, method for separating erythrocytes, and hemagglutination reagent
  • Method for agglutinating erythrocytes, method for separating erythrocytes, and hemagglutination reagent
  • Method for agglutinating erythrocytes, method for separating erythrocytes, and hemagglutination reagent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1~4

[0048] (Examples 1~4, Comparative Examples 1-1~4-2)

[0049] Erythrocyte agglutination based on the presence or absence of bile acid-based surfactants and salts and the pH range

[0050] Add 10 mL of 10% w / v sodium taurodeoxycholate hydrate (TDOC), 10 mL of 1M citric acid, and 9 mL of 10% w / v sodium chloride to 71 mL of distilled water to prepare a final concentration of 100 mM citric acid. acid, 1% TDOC, 0.9% sodium chloride solution (reagent for erythrocyte agglutination). Also, a solution (reagent for erythrocyte agglutination) without adding TDOC and / or sodium chloride was prepared in the same manner, and the influence of the presence or absence of bile acid-based surfactant and salt and the pH range on erythrocyte agglutination were compared.

[0051] 60 μL of the whole blood sample was added to 400 μL of the reagent for erythrocyte agglutination, and the agglutination speed of the erythrocytes was checked visually. The agglutination rate was recorded as +++>++>+>± in o...

Embodiment 5~7

[0062] (Examples 5~7) Erythrocyte agglutination by bile acid-based surfactant and acid

[0063] Various bile acid-based surfactants (1% by weight) and citric acid (100 mM) were added to physiological saline to prepare a reagent for erythrocyte agglutination (pH 3.0), and the agglutination ability of erythrocytes was compared.

[0064] 60 μL of the whole blood sample was added to 400 μL of the reagent for erythrocyte agglutination, and the agglutination rate of erythrocytes was confirmed. The agglutination rate was recorded as +++>++>+>± in order of speed according to the criteria described below, and was recorded as - when hemolysis occurred without agglutination. In addition, the following standard is the same as the standard of said Examples 1-4 and Comparative Examples 1-1-4-2.

[0065] +++: Red blood cells are instantaneously agglutinated to a sufficient size, and the agglutinates of red blood cells are precipitated

[0066] ++: Red blood cells begin to agglutinate after...

Embodiment 8

[0074] (Example 8) Erythrocyte agglutination based on concentration of bile acid-based surfactant

[0075] A reagent for erythrocyte agglutination (pH 3.0) was prepared by adding taurodeoxycholate sodium hydrate (TDOC) and citric acid (100 mM) to physiological saline, and the erythrocytes were compared for the concentration range of TDOC shown in the following table agglutination ability.

[0076] 60 μL of the whole blood sample was added to 400 μL of the reagent for erythrocyte agglutination, and the agglutination rate of erythrocytes was checked. The agglutination rate was recorded as +++>++>+>± in the order of speed according to the following criteria, and it was recorded as - in the case of hemolysis without agglutination. In addition, the following standard is the same as the standard of said Examples 1-7 and Comparative Examples 1-1-4-2.

[0077] +++: Red blood cells are instantaneously agglutinated to a sufficient size, and the agglutinates of red blood cells are prec...

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Abstract

Provided are: a method for agglutinating erythrocytes and a method for separating erythrocytes, wherein erythrocytes can be instantaneously agglutinated into a sufficient size from a blood sample, andthe erythrocytes can be completely separated from the blood sample; and a hemagglutination reagent. The method for agglutinating erythrocytes comprises adding a solution, containing a cholic acid-based surfactant and an acid, to a blood sample. The method for separating erythrocytes comprises separating the erythrocytes agglutinated by the method for agglutinating erythrocytes. In addition, the hemagglutination reagent contains a cholic acid-based surfactant and an acid.

Description

technical field [0001] The present invention relates to a method for agglutination and separation of erythrocytes and a reagent for agglutination of erythrocytes. Background technique [0002] Blood is used for diagnosis and treatment of various diseases, and is composed of blood cell components such as red blood cells, white blood cells, and platelets, and plasma as a liquid component. Plasma (liquid component) contained in the blood or serum obtained by removing blood cell components and fibrin from the blood contains various components necessary for maintaining biological functions such as proteins, sugars, and lipids, and can be used for treating diseases of the internal organs, etc. Analytical samples for diagnostic or therapeutic biochemical examinations. [0003] However, if blood cell components are present in the sample for analysis during a biochemical test, the blood cell component may interfere with the test. The color or turbidity of the object affects the ins...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48
CPCG01N33/80G01N33/5002G01N1/4044G01N33/48
Inventor 村松志野加藤大介服部友洋
Owner DENKA CO LTD
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