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34 results about "Red blood cell agglutination" patented technology

•Clumping (agglutination) of red blood cells is frequently caused by cold agglutinins. Cold agglutinins are IgM antibodies that may arise following viral or Mycoplasma infections, or in the setting of plasma cell or lymphoid neoplasms. Agglutination of red cells can interfere with red blood cell indices.

Mesoporous bioactive glass/chitosan composite hemostatic sponge and preparation method thereof

The invention discloses mesoporous bioactive glass / chitosan composite hemostatic sponge and a preparation method thereof. The method comprises the following steps of preparing mesoporous bioactive glass by using a bicontinuous microemulsion technology in combination with a sol-gel template method, blending a chitosan solution, a polyvinyl alcohol solution and the mesoporous bioactive glass, performing uniform dispersing, adding a cross-linking agent for a reaction, pouring a reaction product into a mold, performing pre-freezing and molding, and performing freeze-drying to obtain the mesoporous bioactive glass / chitosan composite hemostatic sponge. The composite sponge is simple in preparation process; red blood cells are agglutinated through the combination of polycations of a chitosan material and anions on the surface of a blood red cell membrane, platelet aggregation is activated, and thrombin is activated; the mechanical strength, porosity and water absorption rate of the sponge are improved due to the addition of the mesoporous bioactive glass; when being in contact with a wound, the sponge can rapidly absorb liquid, so that the aggregation of platelets and red blood cells is accelerated, blood clots are rapidly formed and accumulated in a sponge network structure with hierarchical pores, and finally the hemostatic effect is achieved; and the sponge has a good application prospect in the field of hemostatic materials.
Owner:SOUTH CHINA UNIV OF TECH +1

Kit for ABO blood type positive typing and Rh blood type detection as well as preparation method and detection method of kit

PendingCN111638373ARealize intuitive interpretationEasy to operateBiological testingRed blood cellBlood type positive
The invention discloses a kit for ABO blood type positive typing and Rh blood type detection as well as a preparation method and a detection method of the kit. The kit comprises a clamping shell whichis wrapped around an outer part, a detection film, a water absorption pad and a back lining that are located in the clamping shell and sequentially arranged from top to bottom. The detection film iscomposed of a character area and a non-character area, and the character area is composed of letters and symbols; the letters comprise letters A, B, O and letters at an Rh position; the letters at theRh position are one or more combinations of C, c, D, E and e; the symbols comprise a minus sign in the middle of the letter O and an plus sign on the upper right side of the letter Rh; each character comprises a hydrophilic area and a hydrophobic area, and the hydrophilic area is a reaction area. According to an immunofiltration principle, red blood cell agglutination is generated by utilizing specific reaction of red blood cell antigens and antibodies, whether red blood cell agglutination colors exist or not is observed, and a text consisting of colors is interpreted to realize blood type detection. The method is easy to operate, a detection result can be visually judged, and blood transfusion accidents caused by misjudgment are prevented.
Owner:长春博德生物技术有限责任公司

Sargassum thunbergii lectin and preparation method thereof

The invention discloses a sargassum thunbergii lectin and a preparation method thereof, wherein the preparation method of the sargassum thunbergii lectin is characterized by selecting sargassum thunbergii as a raw material, carrying out lyophilization, pulverization, immersion, centrifugation, ammonium sulfate fractionation, DEAE-52 ion-exchange chromatography and SephadexG-200 gel filtration chromatography, thus producing the sargassum thunbergii lectin with a molecular weight of 17 kD. The sargassum thunbergii lectin of the invention can agglutinate a wide range of cells, has agglutinative functions for erythrocytes of rabbits, dogs, sheep, crucians, chickens and humans (A, B, AB), and the like, wherein minimum concentrations of agglutination reactions with rabbit and chicken erythrocytes are 4.4mg / ml and 0.55 mg / ml respectively, and shows hemagglutinin activity for rabbit erythrocytes even after heating treatment at 100 DEG C for 30 min (the activity decreases by 75%). A sugar inhibition experiment shows that the sargassum thunbergii lectin is only inhibited by glycoproteins as bovine thyroglobulin and gamma-globulin, and the minimum inhibition concentrations are 1.25 mg.mL<-1> and 2.5 mg.mL<-1> respectively.
Owner:DALIAN OCEAN UNIV +1

Humanized CD47 antibody or antigen binding fragment thereof and application

The invention provides a humanized CD47 antibody or an antigen binding fragment thereof and application, the humanized CD47 antibody or the antigen binding fragment thereof is low in toxicity and efficient, erythrocyte agglutination does not occur in vitro, and erythrocyte removal cannot be caused. In addition, the humanized CD47 antibody provided by the invention shows extremely weak level of low binding or non-binding with platelets and red blood cells, and shows more specific targeting specificity on CD47+ tumor cells. The humanized CD47 antibody or the antigen binding fragment thereof provided by the invention can effectively block the binding of CD47 and SIRP alpha in function, and activate and mediate the phagocytic activity of macrophages to tumor cells.
Owner:BETA PHARM SUZHOU LTD

Immunochromatographic test strip for detecting object in red blood cell-containing sample and immunochromatography using the test strip

A problem of the present invention is to provide an immunochromatographic test strip avoiding agglutination of colloidal gold while red blood cells in whole blood are agglutinated and then separated and removed in the case of using polybrene as a hemagglutinating agent and the colloidal gold conjugates as a detection reagent, and to provide immunochromatography using the test strip. To solve the problem, the present inventors reviewed the composition of the existing reagent itself from a completely different viewpoint rather than the selection of type or amount of polyanions, and as a result of extensive study on each element, the inventors surprisingly found that agglutination of colloidal gold may be suppressed by using a particular additive without neutralization by polyanions.
Owner:SEKISUI MEDICAL CO LTD

Novel coronavirus COVID-2019 detection card and preparation method thereof

The invention discloses a novel coronavirus COVID-2019 detection card and a preparation method thereof. The novel coronavirus COVID-2019 detection card comprises a card supporting layer attached to detection test paper, a sample unit positioned on the card supporting layer, a preprocessing unit positioned on the card supporting layer, a marker unit positioned on the card supporting layer, a detection unit positioned on the card supporting layer, and a recycling unit positioned on the card supporting layer; the sample units correspond to the sample holes; the pretreatment unit corresponds to the pretreatment hole; a hollow or transparent sealed detection window is arranged at the position, corresponding to the detection unit, of the shell; the erythrocyte agglutination layer is provided with erythrocyte agglutinin; the endogenous interfering substance treatment layer is provided with an endogenous interfering substance conjugate; the exogenous interfering substance treatment layer is provided with an exogenous interfering substance chelating agent; a non-specific IgM antibody treatment layer is provided with an IgM chelating agent; a non-specific IgG antibody treatment layer has anIgG precipitant. The problem that interfering substances influence the detection result of the novel coronavirus is solved, and the detection accuracy is improved.
Owner:北京乐普诊断科技股份有限公司

Positive serum national standard substance used for newcastle disease hemagglutination inhibition test and preparation method thereof

The invention relates to a positive serum national standard substance used for a newcastle disease hemagglutination inhibition test and a preparation method thereof. In the positive serum national standard substance used for the newcastle disease hemagglutination inhibition test, Newcastle Disease Virus (NDV) La Sota strain virus and inactivated vaccine immune SPF (Specific Pathoge Free) chicken are utilized to prepare hyperimmune serum to be used as a candidate, through the processes of candidate detection, standard substance preparation, detection, split charging, freeze-drying and the like, the positive serum international standard substance used for the newcastle disease hemagglutination inhibition test obtained by NIBSC (National Institute for Biological Standards and Control) is taken as a reference to carry out a red blood cell agglutination inhibition test to determinate a valence of the standard substance, and through a uniformity test, a stability test, cooperation calibration and data statistics, a constant valence value is ensured to be accurate, and the positive serum national standard substance used for the newcastle disease hemagglutination inhibition test is prepared. The method disclosed by the invention makes the standard substance preparation more scientific, rigorous and perfect according to the standard substance preparation process.
Owner:CHINA INST OF VETERINARY DRUG CONTROL

C-type lectin Nattectin gene of larimichthys crocea, C-type lectin Nattectin recombinant protein of larimichthys crocea and application of C-type lectin Nattectin recombinant protein

The invention provides a C-type lectin Nattectin gene of larimichthys crocea, C-type lectin Nattectin recombinant protein of the larimichthys crocea and an application of the C-type lectin Nattectin recombinant protein, and relates to animal lectins. The amino acid sequence of C-type lectin Nattectin of the larimichthys crocea is represented as SEQ ID NO: 1. The nucleotide sequence of the C-type lectin Nattectin gene of larimichthys crocea is represented as SEQ ID NO: 2. A pET-32a-Nattectin recombinant expression vector is constructed; an escherichia coli expression strain pET-32a-Nattectin-BL21 with high-copy transformants is acquired; fermented cultivation and induced expression of the transformants are performed; recombinant protein Nattectin purification and renaturation are performed. The C-type lectin Nattectin recombinant protein of the larimichthys crocea can be applied to mediated bacteria agglutination and mediated red blood cell agglutination. The bacterial agglutination function and renaturation with one-step desalination method of Nattectin are discovered for the first time, a large amount of soluble bioactive protein is obtained finally, and the operation process is very simple.
Owner:JIMEI UNIV

Monoclonal antibody specifically binding to human CD47 and application thereof

The invention provides a monoclonal antibody specifically binding to human CD47 or a fragment thereof, which can bind to CD47 on the surface of a cell and block binding of SIRP alpha to CD47 on the surface of the cell. The affinity KD value on the recombinant human CD47 is between 1 * 10 <-10 > and 8 * 10 <-8 >, and the tumor inhibition activity and the adverse reaction activity can be balanced. In the aspect of tumor inhibition activity, the antibody can inhibit tumor growth in a CD47 / SIRP alpha double-transgenic mouse tumor-bearing hCD47-MC38 subcutaneous transplanted tumor model, and can enhance in-vivo cell phagocytosis and prolong the lifetime of a mouse in a mouse leukemia model of transplanted human acute B lymphocytic leukemia; in the aspect of adverse reaction activity, the antibody does not have or only has reduced erythrocyte agglutination activity, and does not have a significant effect or only has an over-sexual effect on erythrocytes, platelets and hemoglobin.
Owner:MABWELL (SHANGHAI) BIOSCIENCE CO LTD

A kind of mesoporous bioactive glass/chitosan composite hemostatic sponge and preparation method thereof

The invention discloses a mesoporous bioactive glass / chitosan composite hemostatic sponge and a preparation method thereof. The method comprises: using double continuous microemulsion technology combined with sol-gel template method to prepare mesoporous bioactive glass, blending chitosan solution, polyvinyl alcohol solution, and mesoporous bioactive glass, and adding a crosslinking agent after uniform dispersion The reaction is poured into a mold, pre-frozen and shaped, and then freeze-dried. The preparation process of the composite sponge is simple, and the combination of the polycation of the chitosan material and the anion on the surface of the red blood cell membrane causes red blood cells to agglutinate, activates platelet aggregation, and activates thrombin at the same time, and the mechanical strength of the sponge is improved due to the addition of mesoporous bioactive glass , porosity and water absorption rate, it can quickly absorb liquid when it contacts the wound, and then accelerate the aggregation of platelets and red blood cells, quickly form blood clots and accumulate in the sponge network structure with multi-level pores, and finally play a hemostatic effect. The field of materials has good application prospects.
Owner:SOUTH CHINA UNIV OF TECH +1
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