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Method for wet detection of fingertip blood as well as kit and device thereof

A detection reagent and wet method, applied in the field of fingertip blood-wet method, can solve the problems of deviation of test results, rupture of blood cells, difficult separation of sample size, etc., and achieve the effect of providing accuracy and simple composition.

Active Publication Date: 2018-03-13
SINOCARE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, fingertip blood is whole blood, and the sample size is too small to separate
Moreover, fingertip blood is directly tested by wet method, and there will be two problems: one is that during the reaction process, blood cells rupture, releasing hemoglobin and other components to interfere with the test; Volume of whole blood, under different blood pressure volumes, the amount of serum is different, and the final test results will have large deviations
Therefore, fingertip blood can hardly be used in current wet assays.

Method used

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  • Method for wet detection of fingertip blood as well as kit and device thereof
  • Method for wet detection of fingertip blood as well as kit and device thereof
  • Method for wet detection of fingertip blood as well as kit and device thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1: A device for fingertip blood wetness detection

[0048] Such as figure 1 and 2 , the device for fingertip blood wetness detection includes a fingertip blood sample dilution coagulation area 3, a hemoglobin detection area 4 and a target analyte detection area 5, the fingertip blood sample dilution coagulation area 3, a hemoglobin detection area 4 Connected with the target analyte detection area 5, the fingertip blood suction device 2 and the pipetting device 1 are fixed on the fingertip blood sample dilution and coagulation area to form an integral device.

Embodiment 2

[0049] Embodiment 2, the kit that is used for fingertip blood wet method detection:

[0050] It includes the device for detecting fingertip blood wetness described in Example 1, an agglutination reagent for red blood cells, a detection reagent for hemoglobin and a detection reagent for a target analyte.

Embodiment 3

[0051] Embodiment 3, the detection of triglyceride:

[0052] Adopt the kit described in embodiment 2 to detect,

[0053] In the detection device, the fingertip blood sample dilution and coagulation area 3, the hemoglobin detection area 4 and the target analyte detection area 5 contain corresponding reagents, and the solution formula of each area is as follows:

[0054] Fingertip blood sample dilution and coagulation area 3: the erythrocyte agglutination reagent is physiological saline containing anti-erythrocyte antibody;

[0055] Hemoglobin detection area 4: the detection reagent of hemoglobin is made up of potassium ferricyanide, 50Mm, pH7.0 dipotassium hydrogen phosphate, 0.1% triton;

[0056] Target analyte detection area 5: Triglyceride detection reagent consists of piperazine-1,4-diethanesulfonic acid buffer (pipis buffer) (50mM, pH6.8), LPL (2200U / L), GK (250U / L), POD (1000U / L), magnesium chloride (40mM), sodium cholate (3.5mM), ATP (1.5μM), 4-aminoantipyrine (1mM), ...

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Abstract

The invention belongs to the field of biochemical detection, and particularly relates to a method for wet detection of fingertip blood as well as a kit and a device thereof. According to the detectionmethod provided by the invention, wet detection of fingertip blood is carried out by adopting the method provided by the invention, and red blood cells in a fingertip blood sample are deposited by ared blood cell agglutination reagent in a first solution, so that the blood cells are rapidly condensed, serum and red blood cells are separated, and a target analyte is prevented from being interfered by blood cell rupture. The method comprises the following steps of: performing a second reaction between the fingertip blood sample diluted by the first solution and a second solution containing a hemoglobin detection reagent, detecting the hemoglobin concentration by a colorimetric method, and calculating a packed cell volume; and calculating a correction value of the concentration of the target analyte according to the obtained packed cell volume result. Therefore, the problem of deviation of a final test result caused by different packed cell volumes is avoided, and the accuracy of the test result is provided. The device and the kit for wet detection of fingertip blood, provided by the invention, are simple in structure and widely applicable to detection of various target objects.

Description

technical field [0001] The invention belongs to the field of biochemical detection, and in particular relates to a blood detection method, in particular to a method for fingertip blood wetness detection, a kit and a device thereof. Background technique [0002] In vitro diagnosis, namely IVD (In Vitro Diagnosis), refers to products and services that obtain clinical diagnostic information by testing human samples (blood, body fluids, tissues, etc.) outside the human body, and then determine diseases or body functions. [0003] In vitro diagnostic testing methods can basically be divided into wet method and dry method. The reaction system of wet method is liquid phase, which is the most widely used method. For example, most of the current biochemical analyzers use wet method for testing. The reaction system of the dry method is solid, such as dry chemical test paper. In the current test and analysis, most of the analytes are analytes in serum, so blood cells and serum need to...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N15/05
CPCG01N15/05G01N21/78
Inventor 李宗祥
Owner SINOCARE
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