Biosensor and preparation method and application thereof

A biosensor, reaction chamber technology, applied in the direction of electrochemical variables of materials, etc., can solve problems such as affecting test results, interfering with reactions and tests, etc.

Inactive Publication Date: 2015-09-23
王天星
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] However, the methods reported in the above-mentioned patent documents do not completely solve the problem of hematocrit sensitivity. These technical means still have some red blood cells passing through the micropores of the membrane or the network structure of the polymer, entering the reaction zone and contacting the electrodes. , thus interfering with the reaction and test; for some personnel or samples, the size of red blood cells is relatively small, and it is easier to pass through the micropores of the membrane or the network structure of the polymer, which greatly affects the test results

Method used

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  • Biosensor and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0069] Such as Figure 1A and Figure 1B Shown, a kind of biosensor, composition comprises insulating base material layer, and insulating base material is PET, is attached to the electrode system on the insulating base material layer, and described electrode system comprises a working electrode and a counter electrode, and electrode material is graphite , each electrode in the electrode system has an extension wire and a contact area, is connected to the tester through the contact area, covers the reagent layer on the electrode system, the reagent layer covers the working electrode, and is located between the electrode system and the tester. The reaction chamber above the reagent layer, the reaction chamber is composed of a space layer and a cover layer, the thickness of the space layer is 0.125mm, the cover layer adopts the 9971 hydrophilic film of 3M Company, and the reaction chamber has air holes for the entry of samples , wherein: the components of the reagent layer contai...

Embodiment 2

[0087] Such as Figure 2A and Figure 2B As shown, a biosensor is composed of an insulating substrate layer, the insulating substrate is acrylic, and an electrode system attached to the insulating substrate layer, the electrode system includes a working electrode and a counter electrode, and the electrode material is graphite , each electrode in the electrode system has an extension wire and a contact area, connected to the tester through the contact area, covering the reagent layer on the electrode system, the reagent layer covers the working electrode, and adheres to the reagent layer The upper omentum layer, and the reaction chamber located above the electrode system and the reagent layer, the reaction chamber is composed of a space layer and a covering layer, the thickness of the space layer is 0.125mm, and the covering layer adopts 9971 hydrophilic membrane of 3M Company, And the reaction chamber has air holes for the entry of samples, wherein: the omentum layer is the o...

Embodiment 3

[0096] The structure and process of this embodiment are the same as in Example 1, but the components of the reagent solution are different, and the composition is as follows:

[0097] Disodium hydrogen phosphate dihydrate: 1.1395g

[0098] Sodium dihydrogen phosphate dihydrate: 2.1223g

[0099] Sodium carboxymethylcellulose (Sigma C5678): 1.0000g

[0100] Gelatin (Sigma G7041): 0.0500g

[0101] Triton X-100: 0.2000g

[0102] Polyethylene glycol (Sigma 95904): 0.5000g

[0103] Potassium ferricyanide: 3.2900g

[0104] Glucose oxidase (Amano, GO-AM): 600KU

[0105] Anti-human erythrocyte antibody: 0.01g

[0106] Purified water: 92.000g,

[0107] Stir the reagent solution containing lectin to fully dissolve it; then add purified water to make up to 100mL, continue stirring until the components are uniform, and set aside.

[0108] Then, according to the preparation method of Example 1, a biosensor was fabricated.

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Abstract

The invention relates to the field of biological detection instruments, in particular to a biosensor. The biosensor comprises a reagent layer. The reagent layer contains red blood cell agglutinant or an omentum layer adhering to the reagent layer. The omentum layer is treated through the red blood cell agglutinant. The invention further provides a preparation method and application of the biosensor. As the problem of the sensibility of the hematocrit value is not solved completely in the prior art, the problem of the sensibility of the hematocrit value is solved by reducing the sensibility of the hematocrit value; accordingly, the accuracy of testing results is improved, and interference of red blood cells on the testing results is reduced to the maximum extent.

Description

technical field [0001] The invention relates to the field of biological detection instruments, in particular to a biosensor and its preparation method and application. Background technique [0002] A biosensor is an instrument that is sensitive to biological substances and converts its concentration into an electrical signal for detection. It is made of immobilized biosensitive materials as recognition elements (including enzymes, antibodies, antigens, microorganisms, cells, tissues, nucleic acids and other biologically active substances), appropriate physical and chemical transducers (such as oxygen electrodes, photosensitive tubes, field effect tubes, Piezoelectric crystals, etc.) and an analysis tool or system composed of a signal amplification device. [0003] Electrochemical biosensors have been widely used in the field of in vitro diagnostics. These instrument systems can detect physiological substances in body fluids, which is convenient for doctors to analyze and di...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26
Inventor 王天星
Owner 王天星
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