3F3Rmg recombinant fusion protein and preparation method thereof and application thereof to detection of dog rabies antibody

Abstract

The invention discloses a 3F3Rmg recombinant fusion protein and a preparation method thereof. The recombinant fusion protein is encoded by a 3F3Rmg fusion gene obtained by splicing a 3F3ScFv gene and an Rmg gene, and has a base sequence shown in a sequence table SEQ.ID.No.1. Due to the difunctional characteristic of the 3F3Rmg recombinant fusion protein, the fusion protein can be combined with a human red blood cell without being agglutinated, can react with a rabies G antibody, and undergoes an agglutination phenomenon which can be observed by naked eyes under an antibody bridging action. A dog rabies antibody red cell agglutination test diagnostic reagent kit prepared by applying the recombinant fusion protein is easy to operate, has high sensitivity and high specificity, is particularly suitable for detecting the dog rabies antibody, and meets the requirements of use rapidness, easiness and convenience for a substrate field.

Description

technical field [0001] The invention relates to the field of prevention and diagnosis of canine rabies virus, in particular to a 3F3Rmg recombinant fusion protein, its preparation method and its application in the detection of canine rabies virus. Background technique [0002] Rabies is a zoonotic disease caused by rabies virus (Rabies virus, RV) and all warm-blooded animals are susceptible to it. Once onset, the fatality rate is almost 100%. It is one of the acute infectious diseases with the highest fatality rate in humans. The glycoprotein (glycoprotein, GP) encoded by the G gene of rabies virus is the only protein exposed on the surface of the virus, and it is also the only protective antigen that can stimulate the body to produce neutralizing antibodies and cellular immunity. The domain of cell receptor binding, and related to virus virulence, plays a key role in rabies virus pathogenesis and immunity. At present, there is no effective drug for the treatment of rabies,...

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Problems solved by technology

[0003] The detection methods for serum rabies virus antibodies mainly include fluorescent antibody test (FAT), enzyme-linked immunosorbent assay (ELISA), rapid fluorescent focus inhibition test (RFFIT), fluorescent antibody virus neutralization test (FAVNT), mouse neutralization However, these detection methods have high requirements and need to be equipped with special instruments and professional technicians, the detection cost is expensive, and it takes a relatively long time, which greatly limits its application in grassroots units.

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