Kit for ABO blood type positive typing and Rh blood type detection as well as preparation method and detection method of kit
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A technology of ABO blood type and detection method, applied in biological testing, measuring devices, material inspection products, etc., can solve problems such as interpretation errors, achieve intuitive interpretation, avoid blood transfusion accidents, and be easy to carry
Pending Publication Date: 2020-09-08
长春博德生物技术有限责任公司
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This detection method needs to be analyzed by professionally trained technicians to determine the blood type. However, even with professionally trained technicians, this method still has the risk of misinterpretation, and it is prone to blood transfusions caused by misinterpretation. ACCIDENT
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preparation example Construction
[0047] The preparation of the symbols on the detection film can be printed by an inkjet printer, or can be drawn by hand or printed with a font. The embodiment of the present invention uses an inkjet printer to print.
[0048] The flushing solution is physiological saline or phosphate buffer saline with isotonic properties.
Embodiment 1
[0050] The determination of embodiment 1 hydrophobic solution
[0051] Prepare 0.3%, 0.5%, 0.7%, 0.9%, and 1% AKD solutions with absolute ethanol, add a drop of hydrophobic solution to the glass fiber membrane, and dry it at room temperature or at 37°C after the diffusion is complete. After the membrane is completely dry, drop a colored liquid solution on it, and observe whether the solution is absorbed by the membrane to determine that the AKD concentration of the solution is 0.5-1%.
Embodiment 2
[0052] Embodiment 2 is used for the preparation of the test kit of ABO blood group positive typing and Rh blood group detection
[0053] The kit contains a reagent card, a blood collection tool and a rinsing solution, wherein the reagent card includes a detection membrane 1, an absorbent pad 2 and a backing 3 that are wrapped in an outer cartridge and located inside the cartridge from top to bottom;
[0054] Taking the kit for ABO blood type positive typing and RhD blood type detection as an example, the letter at the Rh position is D, and the vertical line with the plus sign on the upper right of the D letter is coated with an anti-D antibody. The preparation method of the detection membrane includes the following steps:
[0055] S1. Prepare A, B, O, and D on the computer in advance + In the graphic design of the character shape, ABO and D can be in the same row of reaction areas, or they can be designed as different rows of reaction areas, such as figure 1 and figure 2 sh...
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Abstract
The invention discloses a kit for ABO blood type positive typing and Rh blood type detection as well as a preparation method and a detection method of the kit. The kit comprises a clamping shell whichis wrapped around an outer part, a detection film, a water absorption pad and a back lining that are located in the clamping shell and sequentially arranged from top to bottom. The detection film iscomposed of a character area and a non-character area, and the character area is composed of letters and symbols; the letters comprise letters A, B, O and letters at an Rh position; the letters at theRh position are one or more combinations of C, c, D, E and e; the symbols comprise a minus sign in the middle of the letter O and an plus sign on the upper right side of the letter Rh; each character comprises a hydrophilic area and a hydrophobic area, and the hydrophilic area is a reaction area. According to an immunofiltration principle, red blood cell agglutination is generated by utilizing specific reaction of red blood cell antigens and antibodies, whether red blood cell agglutination colors exist or not is observed, and a text consisting of colors is interpreted to realize blood type detection. The method is easy to operate, a detection result can be visually judged, and blood transfusion accidents caused by misjudgment are prevented.
Description
technical field [0001] The invention relates to the technical field of in vitro detection, in particular to a kit for detecting ABO blood type and Rh blood type, a preparation method and a detection method thereof. Background technique [0002] At present, ABO blood typing and RhD blood typing are required before clinical blood transfusion. On this basis, the detection of other antigens of the Rh system is also particularly necessary. ABO blood typing includes two aspects, positive stereotyping and negative stereotyping. Positive typing refers to erythrocyte typing, which refers to the use of known specific anti-A / anti-B blood group typing reagents to detect the presence of corresponding antigens on red blood cells; reverse typing refers to serum typing, which refers to the detection of type A / B red blood cells with known antigens to be tested Whether there are corresponding antibodies in serum or plasma. Combining positive and negative typing results, the blood type of th...
Claims
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