Method for reducing water eutrophication by chlorella culture

A technology for eutrophication and chlorella in water body, applied in chemical instruments and methods, water/sewage treatment, biological water/sewage treatment, etc., can solve the problems of complex phytoplankton community structure, etc. The effect of promoting chlorophyll and bacterial protein accumulation

Inactive Publication Date: 2019-08-02
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the actual eutrophic water body, the phytoplankton community structure is very complex, not ...

Method used

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  • Method for reducing water eutrophication by chlorella culture
  • Method for reducing water eutrophication by chlorella culture
  • Method for reducing water eutrophication by chlorella culture

Examples

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Embodiment 1

[0033] A method for domesticating chlorella, exposing the chlorella to intense pulsed light radiation during the domestication process. The method for acclimating Chlorella is as follows: inoculate algae cells in nutrient-rich water according to 5% of the working volume, and cultivate them for 3 days at a temperature of 20°C, a light intensity of 2000Lux, and a light-dark ratio of 12h:12h after being irradiated by intense pulsed light. After five batches of heterotrophic domestication, the heterotrophic domesticated algae are separated and purified to obtain the domesticated chlorella. Chlorella has good nitrogen and phosphorus removal ability and pollution resistance, high growth rate, and can be used as the dominant algal species for nitrogen and phosphorus removal.

[0034] Wherein, the wavelength of the intense pulsed optical radiation is 570nm, the pulse duration is 3ms, and the pulse delay is 20ms. The photothermolysis and photochemical effects of intense pulsed light r...

Embodiment 2

[0042] A method for domesticating Chlorella is as follows: inoculate algae cells in nutrient-rich water according to 10% of the working volume, and irradiate with intense pulsed light with a wavelength of 600nm, a pulse duration of 3.5ms, and a pulse delay of 30ms The temperature was 27°C, the light intensity was 2600 Lux, and the light-dark ratio was 12h:12h and cultured for 3.5 days. After 6 batches of heterotrophic domestication, the heterotrophic domesticated algae were separated and purified to obtain the domesticated Chlorella.

[0043] A method for reducing eutrophication in water bodies by using chlorella culture, using the domesticated chlorella obtained by the method for domesticating chlorella. Methods to reduce eutrophication in water bodies include,

[0044] 1) The nutrient-rich water body is pretreated by adding a flocculant, the dosage of the flocculant is 0.85g / L, and the flocculant is medical stone flocculant;

[0045] 2) Put the domesticated chlorella into t...

Embodiment 3

[0048] A method for domesticating Chlorella is as follows: inoculate algae cells in nutrient-rich water body according to 15% of the working volume, irradiate with intense pulsed light with a wavelength of 640nm, a pulse duration of 4ms, and a pulse delay of 40ms. The temperature was 30°C, the light intensity was 3000 Lux, and the light-to-dark ratio was 12h:12h and cultured for 4 days. After 8 batches of heterotrophic domestication, the heterotrophic domesticated algae were separated and purified to obtain the domesticated Chlorella.

[0049] A method for reducing eutrophication in water bodies by using chlorella culture, using the domesticated chlorella obtained by the method for domesticating chlorella. Methods to reduce eutrophication in water bodies include,

[0050] 1) The nutrient-rich water body is pretreated by adding a flocculant, the dosage of the flocculant is 1.0g / L, and the flocculant is medical stone flocculant;

[0051] 2) Put the domesticated chlorella into t...

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Abstract

The invention provides a method for reducing water eutrophication by chlorella culture, and belongs to the technical field of eutrophic water restoration. The method comprises steps as follows: eutrophic water is pretreated by adding a flocculating agent; domesticated chlorella is added to the eutrophic water and allowed to grow naturally, and dead chlorella is picked out. A chlorella domesticating method comprises the step that the chlorella is exposed in intense pulsed light radiation in a chlorella domestication process, wherein the flocculating agent is a medical stone flocculating agent,and 50% or more of the overall flocculating agent has the particle size smaller than 10 mu m. By means of the method for reducing water eutrophication by chlorella culture, nutrient substances such asnitrogen and phosphorus in the eutrophic water can be rapidly removed, dominant species of colonies and diversity of species are changed, ecological restoration of the eutrophic water is realized, and besides, chlorophyll and mycoprotein accumulation in the later period of the domesticated chlorella can be promoted.

Description

technical field [0001] The invention belongs to the technical field of eutrophic water body restoration, and in particular relates to a method for reducing eutrophication of water body by cultivating chlorella. Background technique [0002] Eutrophication of water body means that a large amount of plant growth elements such as nitrogen and phosphorus are discharged into the water body, causing the growth and reproduction of algae and other aquatic organisms in large quantities. At the same time, the rate of organic matter production far exceeds the rate of consumption. balancing process. With the development of social economy and the continuous improvement of people's living standards, the people's demand for the types and quantities of aquatic products is increasing. It is of great significance to develop aquaculture such as ponds. As a relatively closed water body, the pond is not smooth in the process of aquaculture. At the same time, with the continuous acceleration of ...

Claims

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Application Information

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IPC IPC(8): C02F3/32C02F9/14
CPCC02F1/50C02F1/5236C02F3/322C02F9/00C02F2001/007C02F2103/007
Inventor 丁月晗张建设鲁敏
Owner ZHEJIANG OCEAN UNIV
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