SNP molecular marker related to chicken body weight, leg muscle weight and leg muscle fiber trait, and detection method and application of SNP molecular marker

Abstract

The invention provides an SNP molecular marker related to chicken body weight, leg muscle weight and leg muscle fiber trait, and a detection method and application of the SNP molecular marker. The nucleotide sequence of the molecular marker is shown as SEQ ID NO: 1, and the 534th position of SEQ ID NO:1 is A or G. The invention also provides a detection primer for the SNP molecular marker relatedto chicken body weight, leg muscle weight, and leg muscle fiber trait; the detection primer comprises a forward primer shown as SEQ ID NO: 2, and a reverse primer shown as SEQ ID NO: 3. Through designa specific primer to amplify a gene fragment containing an SNP site, and detect the genotype of the SNP site of a chicken to be tested, the single nucleotide polymorphism can be simply, quickly, andaccurately detected with low cost, production costs can be reduced and genetic development can be accelerated, thus better serving the breeding of high-quality chickens, and realizing great economic and scientific research values.

Description

technical field [0001] The invention belongs to the technical field of poultry genetic engineering, and more specifically relates to a SNP molecular marker, detection primers, detection kit and application related to chicken body weight, leg muscle weight and leg muscle fiber traits. Background technique [0002] Due to its high protein, low cholesterol, low fat and low calorie characteristics, chicken has become a high-quality animal protein source generally accepted by consumers all over the world. In addition, compared with pigs and cattle, broiler chickens have the advantages of short feeding cycle, high feed conversion rate, and high degree of automation in feeding and processing. Therefore, chicken is also the most economical source of animal protein. At present, although chicken has become the second largest meat consumption product in my country after pork, the per capita consumption of chicken is still far below the world's developed countries and the international ...

AI Technical Summary

Problems solved by technology

SNP molecular markers that are simultaneously associated with chicken body weight, leg muscle weight and leg muscle fiber traits have not been found in the prior art

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