Pediococcus acidilactici and application thereof
A technology of Pediococcus lactis, JY03, applied in the application field of Pediococcus lactis, can solve the problems of microbial drug resistance, chemical bactericide residues, etc., achieve the effect of enriching the resource library of bacteria species and broadening the scope of application
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[0014] In order to make the object, technical solution and advantages of the present invention clearer, preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.
[0015] 1. Screening of lactic acid bacteria
[0016] Citrus fruits, leaves, soil and peanut leaves were collected from Jinyun Mountain Citrus Orchard in Beibei District, Chongqing as samples. Each sample was completely submerged in 100mL MRS broth medium respectively, enriched and cultured at 37°C for 24 hours, and spread 10-fold serially diluted on a medium containing 0.3% CaCO 3 On the MRS plate, cultured at 37°C for 48 hours, a single colony with obvious calcium lysis circle was selected for isolation and purification, and microscopic examination and Gram staining were carried out. The purified strains were stored in a -80°C refrigerator.
[0017] The results are shown in Table 1. A total of 68 suspected strains were screened out, including 16 ...
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