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A lactic acid bacterium lactobacillus sucicola and its application

A technology of lactic acid bacteria and Penicillium digitatum, which is applied in the field of lactic acid bacteria, can solve the problems of chemical bactericide residue and microbial resistance, and achieve the effect of broadening the application range and enriching the strain resource bank

Active Publication Date: 2020-12-08
SOUTHWEST UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, chemical fungicides such as carbendazim, thiophanate-methyl, and imazalil are mainly used in the prevention and control of postharvest diseases of citrus fruits, but there are problems such as the residue of chemical fungicides and the tendency of microorganisms to develop resistance to them.

Method used

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  • A lactic acid bacterium lactobacillus sucicola and its application
  • A lactic acid bacterium lactobacillus sucicola and its application
  • A lactic acid bacterium lactobacillus sucicola and its application

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Embodiment Construction

[0014] In order to make the object, technical solution and advantages of the present invention clearer, preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0015] 1. Screening of lactic acid bacteria

[0016] Citrus fruits, leaves, soil and peanut leaves were collected from Jinyun Mountain Citrus Orchard in Beibei District, Chongqing as samples. Each sample was completely submerged in 100mL MRS broth medium respectively, enriched and cultured at 37°C for 24 hours, and spread 10-fold serially diluted on a medium containing 0.3% CaCO 3 On the MRS plate, cultured at 37°C for 48 hours, a single colony with obvious calcium lysis circle was selected for isolation and purification, and microscopic examination and Gram staining were carried out. The purified strains were stored in a -80°C refrigerator.

[0017] The results are shown in Table 1. A total of 68 suspected strains were screened out, including 16 ...

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Abstract

The invention discloses a lactic acid bacterium Lactobacillus sucicola JT03 with a preservation number of CGMCC No. 17538, and the use of the lactic acid bacterium in preparing medicines for inhibiting Penicillium digitatum (Penicillium digitatum), especially for preventing postharvest green mold of citrus fruits caused by Penicillium digitatum application in medicines. The invention not only enriches the resource bank of lactic acid bacteria strains, broadens the application range of lactic acid bacteria in the field of fresh-keeping of fruits and vegetables, but also provides a new solution for the prevention and treatment of postharvest green mold of citrus fruits.

Description

technical field [0001] The invention relates to a strain of lactic acid bacteria, and also relates to the application of the lactic acid bacteria. Background technique [0002] Lactic acid bacteria are a class of Gram-positive bacilli or cocci that can ferment sugars and whose main product is lactic acid. They have a good inhibitory effect on some Gram-positive or negative bacteria that are closely related to them. They are currently widely used in food fermentation, Fresh-keeping and other fields. However, there are few reports about the inhibitory effect of lactic acid bacteria on fungi. [0003] Citrus is a plant of the Citrus subfamily of Rutaceae. Citrus fruit is the fruit with the largest output in the world. During storage, it is prone to rot, edema, floating skin and other phenomena due to microbial infection and storage environment, which seriously affects the commercial value of the fruit. . Green mold caused by the fungus Penicillium digitatum is the main infec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00C12R1/225
CPCA01N63/00C12N1/205C12R2001/225
Inventor 曾凯芳马佳宏易兰花邓丽莉姚世响阮长晴
Owner SOUTHWEST UNIV
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