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A Soil Proteus and Its Application

A proteobacteria and soil technology, applied in the field of environmental pollution remediation, can solve the problems of substrate diversity and increase the difficulty of developing biodegradation technology, and achieve the effect of stable degradation performance and wide application prospects.

Active Publication Date: 2022-04-08
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the combined pollution of pesticides and antibiotics contains a variety of pollutants, their physical and chemical properties, bioaffinities, etc. are different, and the diversity of substrates may increase the difficulty of the development of biodegradation technology
The Chinese invention patent with the publication number CN106434430A provides a compound microbial bacterial agent that can degrade antibiotics and pesticide residues, but at present, there are few reports on single bacterial strains for compound pollution of pesticides and antibiotics, and the screening of active strains and the maintenance of degradation activity It is the core difficulty of bioremediation of complex pollution in farmland, and it is also an important basis for the practice and promotion of bioremediation technology

Method used

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  • A Soil Proteus and Its Application
  • A Soil Proteus and Its Application
  • A Soil Proteus and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Example 1 Isolation, identification and preservation of strains

[0053] 1. Isolation and purification of strains

[0054] Soil samples were collected from the farmland system in Boluo City, Guangdong Province, and 5 g was placed in a 250 mL Erlenmeyer flask containing 50 mL of basal salt medium. Maintain a constant temperature of 30 °C, acclimatize and cultivate for 5 d on a shaker at 180 r / min. Set 4 gradients of tetracycline concentration as the acclimation culture concentration (25, 50, 75, 100 mg / L), and transfer 5 mL of inoculum to the medium of the next concentration every 5d until the concentration is 100 mg / L.

[0055] Take 1mL of the acclimated bacterial liquid at a concentration of 100mg / L tetracycline and transfer it into 9mL of normal saline, and dilute it into 10-2, 10-3, 10-4, 10-5, 10-6, 10-7, 10 After -8, take 100uL of the liquid in 10-5 to 10-8 as the inoculum and spread it on LB solid medium. After 5 days, single colonies on the plate were picked a...

Embodiment 2

[0072] The degradation effect experiment of embodiment 2 strain on tetracycline

[0073] 1. Experimental method

[0074] (1) Preparation of inoculum

[0075] Take 0.2 mL of the cryopreserved degrading bacteria ZQ02 bacterial solution, transfer and inoculate it into a conical flask containing 50 mL of fresh LB medium. Take 25 mL of bacterial liquid into a sterilized centrifuge tube, centrifuge at 5000 r / min for 5 min to collect the bacterial cells, and wash twice with 25 mL of sterile saline. Finally, sterilized saline was added and shaken to make the inoculum OD. 600 A value of 1 is prepared as an inoculum.

[0076] (2) Determination of degradation performance

[0077] The tetracycline stock solution was added to the conical flask to make the initial concentration 50mg / L, pH value 7, and the degrading bacteria inoculum was added to the inoculum at 5% by volume, and a sterile blank control was established. Each treatment group was repeated 3 times. The temperature was set ...

Embodiment 3

[0085] Example 3 Optimal degradation conditions of tetracycline by strain ZQ02

[0086] 1. Experimental method

[0087] (1) Factors influencing the degradation of tetracycline by degrading bacteria

[0088] This single-factor experiment mainly studies the effects of temperature, initial pH value, inoculum size, and initial concentration on the biodegradation of tetracycline. The treatment groups of each influencing factor are: culture temperature (20℃, 25℃, 30℃, 35℃, 40℃); pH value (5, 6, 7, 8, 9); initial concentration (5, 10, 25 , 50, 75, 100 mg / L); inoculation amount (1%, 3%, 5%, 7%, 9%).

[0089] (2) Response surface optimization design

[0090] In order to clarify the bioremediation potential of tetracycline by degrading bacteria ZQ02 (Proteus terrae), the response surface method was used to optimize the influence of various factors and their interactions. Based on the Box-Behnken design, the main influencing factors (cultivation temperature, pH value, inoculum amount...

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Abstract

The invention discloses a soil proteus strain and application thereof. The soil Proteus terrae ZQ02 strain was preserved in the Guangdong Provincial Microbiological Culture Collection Center on November 01, 2019, with the preservation number GDMCC NO: 18782, and the preservation address is: 5th Floor, Building 59, Compound, No. 100 Xianlie Middle Road, Guangzhou City . The degradation rate of the strain to tetracycline can reach 90.54%, and the degradation rate to chlorothalonil can reach 96.84%. It has the advantages of high efficiency, rapidity, no residue and no secondary pollution in degrading tetracycline and chlorothalonil and other antibiotics and pesticides. And the degradation performance is stable, and it has broad application prospects for ecological restoration of pesticide-antibiotic compound pollution.

Description

technical field [0001] The invention belongs to the technical field of environmental pollution remediation, and more particularly, relates to a soil Proteus and its application. Background technique [0002] The scientific use of agrochemicals such as pesticides, fertilizers and antibiotics is an important basis for ensuring the sustainable development of modern agricultural production. my country is a big country in the use of pesticides and antibiotics. The phenomenon of long-term excessive and frequent use of agrochemicals in my country is common, resulting in a new feature of the agricultural ecological environment with the combined pollution of pesticides and antibiotics, becoming one of the main sources of agricultural non-point source pollution. The soil ecology is stable, and it also accumulates in the edible parts of crops through plant systemic transport, which can easily lead to human health risks. In the face of increasingly stringent agricultural product safety ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A62D3/02C12R1/37A62D101/22A62D101/26A62D101/28A62D101/04
CPCC12N1/20A62D3/02A62D2101/04A62D2101/26A62D2101/28A62D2101/22
Inventor 刘婕钟国华朱祺卢琪琪李锦鸿
Owner SOUTH CHINA AGRI UNIV
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