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Method and kit for detecting infection lines based on high-throughput sequencing

A high-throughput, line-based detection technology, applied in the field of high-throughput sequencing, can solve problems affecting sensitivity and other issues, and achieve high-sensitivity and high-accuracy detection results

Active Publication Date: 2019-08-06
GENESEEQ TECH INC +1
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0006] However, for the detection of pathogen infection, the sample contains a large number of DNA fragments from the human body. Since the human genome is far larger than the genome of pathogenic microorganisms, human genome fragments will seriously affect the sensitivity of detection of pathogenic microorganism DNA. Therefore, the removal of human genome The impact of fragments is critical for improving the sensitivity and specificity of high-throughput sequencing for the detection of pathogenic microorganisms

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  • Method and kit for detecting infection lines based on high-throughput sequencing
  • Method and kit for detecting infection lines based on high-throughput sequencing
  • Method and kit for detecting infection lines based on high-throughput sequencing

Examples

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Embodiment 1

[0092] In this example, the detection of Klebsiella pneumoniae (bacteria) as a potential pathogen is exemplarily illustrated.

[0093] In order to avoid the influence of human DNA, an additional reverse enrichment step is added in the high-throughput sequencing process, mainly for probe design on human genomic DNA fragments with high repeatability that will affect the sensitivity of the detection process. Capture and remove human DNA fragments.

[0094] The human genome includes protein-coding sequences and non-coding sequences, and protein-coding sequences only account for about 1.5% of the genome size. At present, there are two main types of commercially available probes, one is the whole exome probe, which covers the exons of more than 20,000 human genes, and can remove protein coding sequences; the other is the customized probe combination (Panel ), covering a range from dozens of genes to hundreds of genes. However, the coverage of whole-exome probes and panels only acc...

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Abstract

The invention relates to a method for detecting infection lines based on high-throughput sequencing, and belongs to the technical field of high-throughput sequencing. The detection method is used fordetecting detection and identification of pathogens in human body samples. In the method, through designing of probes for capturing the pathogens, known and unknown pathogens can be effectively detected. Meanwhile, probes for specific hybrid combination with highly repetitive fragments of human genes can be designed, and the interference of human DNA fragments to the sensitivity of the detection process can be effectively avoided.

Description

technical field [0001] The invention relates to a method for detecting infection lines based on high-throughput sequencing, and belongs to the technical field of high-throughput sequencing. Background technique [0002] Emerging infectious diseases are an important threat to human beings. Population growth, rapid urbanization, changes in the natural ecological environment, increased opportunities for contact between humans and wild animals, economic globalization, and the surge in the number of people traveling across borders, The accelerated speed of cross-border travel and the overuse of antibiotics have greatly increased the risk of emerging infectious diseases. According to the research report of WHO, since 1967, at least 39 new pathogens have been discovered, important ones include HIV, Ebola virus, Marburg virus, SARS virus, bird flu virus and swine flu virus. New diseases are emerging at unprecedented rates (on average one new per year) and spreading across borders a...

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Application Information

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IPC IPC(8): C12Q1/6869C12Q1/689C12N15/11C12R1/22
CPCC12Q1/6869C12Q1/689C12Q2525/191C12Q2565/519C12Q2535/122C12Q2563/131
Inventor 邵阳那成龙刘佳郑佳方圆蒋思明汪笑男
Owner GENESEEQ TECH INC