Method for preparing nanometer zinc oxide based on Lactobacillus casei secretion

A technology of Lactobacillus casei and nano-zinc oxide, which is applied in the field of preparing nano-zinc oxide, can solve the problems of low purity of nano-zinc oxide, and achieve the effects of low toxicity, high yield and high purity
CN110104673AActive Publication Date: 2019-08-09NORTHWESTERN POLYTECHNICAL UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NORTHWESTERN POLYTECHNICAL UNIV
Publication Date
2019-08-09

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Abstract

The invention discloses a method for preparing nanometer zinc oxide based on Lactobacillus casei secretion. The method is used for solving the technical problem of low purity of nanometer zinc oxide prepared by existing methods. The method comprises the following steps: preparing an intermediate Zn(OH)2 by using anhydrous zinc acetate as a substrate and using Lactobacillus casei L. Casei ATCC 393as a biological medium, magnetically stirring the intermediate Zn(OH)2 at a constant temperature, and then calcining the stirred intermediate at a high temperature to obtain nano-ZnO. Compared with the background art, the method in the invention has the following advantages: the anhydrous zinc acetate is less toxic than ZnCl2; the Lactobacillus casei L. Casei ATCC 393 is safer than Lactobacillus saliva L3; and the preparation of nano-ZnO is based on the fermentation product of the L. casei ATCC 393, rather than the bacterium itself, so the separation and purification process is simplified, andthe yield and purity of the prepared product nano-ZnO are high.
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Description

technical field

[0001] The invention relates to a method for preparing nano zinc oxide, in particular to a method for preparing nano zinc oxide based on the secretion of Lactobacillus casei. Background technique

[0002] The document "Studies on the Biosynthesis of Nano-zinc Oxide by Lactobacillus salivarius L3" Modern Food Science and Technology, No. 9, 2013, Page 2192-2198" discloses a preparation method of nano-zinc oxide. The biological medium used in this method is from weaning Lactobacillus salivarius L3 isolated from piglet feces. The preparation process is to adjust the pH of the culture medium after the fermentation of Lactobacillus salivarius L3 is completed, and add an appropriate concentration of ZnCl 2 The solution was incubated in a water bath for a certain period of time, and aged at 37°C for 12 hours. The product was collected by centrifugation, washed three times, and dried to constant weight, which was nano-ZnO. However, the ZnCl used in this production pr...

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