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Method for preparing nanometer zinc oxide based on Lactobacillus casei secretion

A technology of Lactobacillus casei and nano-zinc oxide, which is applied in the field of preparing nano-zinc oxide, can solve the problems of low purity of nano-zinc oxide, and achieve the effects of low toxicity, high yield and high purity

Active Publication Date: 2019-08-09
NORTHWESTERN POLYTECHNICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to overcome the low purity of nano-zinc oxide prepared by existing methods, the invention provides a method for preparing nano-zinc oxide based on the secretion of Lactobacillus casei

Method used

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  • Method for preparing nanometer zinc oxide based on Lactobacillus casei secretion
  • Method for preparing nanometer zinc oxide based on Lactobacillus casei secretion
  • Method for preparing nanometer zinc oxide based on Lactobacillus casei secretion

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Step 1. Strain activation:

[0019] Lactobacillus casei L.casei ATCC 393 strain was activated and cultured with MRS solid medium. The formula of MRS solid medium was as follows: peptone 10g / L, beef extract 10g / L, yeast extract 5g / L, diammonium hydrogen citrate 2g / L L, glucose 20g / L, crystalline sodium acetate 5g / L, dipotassium hydrogen phosphate 2g / L, magnesium sulfate heptahydrate 0.6g / L, manganese sulfate monohydrate 0.25g / L, Tween 801mL / L, agar 15g / L , pH 6.4; L.casei ATCC 393 was inoculated in MRS solid medium by streaking, and cultured anaerobically at 37°C for 48h.

[0020] Step 2, biosynthesis of zinc hydroxide:

[0021] Cultivate the single colony of Lactobacillus casei L.casei ATCC 393 picked with MRS liquid medium. The formula of MRS liquid medium is as follows: peptone 10g / L, beef extract 10g / L, yeast extract 5g / L, dihydrogen citrate Ammonium 2g / L, glucose 20g / L, crystalline sodium acetate 5g / L, dipotassium hydrogen phosphate 2g / L, magnesium sulfate heptahy...

Embodiment 2

[0032] Step 1. Strain activation:

[0033] Lactobacillus casei L.casei ATCC 393 strain was activated and cultured with MRS solid medium. The formula of MRS solid medium was as follows: peptone 11g / L, beef extract 11g / L, yeast extract 4g / L, diammonium hydrogen citrate 1.5g / L, glucose 23g / L, crystalline sodium acetate 4g / L, dipotassium hydrogen phosphate 2.3g / L, magnesium sulfate heptahydrate 0.5g / L, manganese sulfate monohydrate 0.27g / L, Tween 80 1.3mL / L, Agar 18g / L, pH 6.2; Streak inoculation of L.casei ATCC 393 on MRS solid medium, culture anaerobically at 35°C for 36h;

[0034] Step 2, biosynthesis of zinc hydroxide:

[0035] Cultivate the single colony of Lactobacillus casei L.casei ATCC 393 picked with MRS liquid medium, the formula of MRS liquid medium is as follows: peptone 11g / L, beef extract 11g / L, yeast extract 4g / L, dihydrogen citrate Ammonium 1.5g / L, glucose 23g / L, crystalline sodium acetate 4g / L, dipotassium hydrogen phosphate 2.3g / L, magnesium sulfate heptahydr...

Embodiment 3

[0039] Step 1. Strain activation:

[0040] Use MRS solid medium to activate Lactobacillus casei L.casei ATCC 393 strain. The formula of MRS solid medium is as follows: peptone 12g / L, beef extract 12g / L, yeast extract 6g / L, diammonium hydrogen citrate 2.5g / L, glucose 25g / L, crystalline sodium acetate 6g / L, dipotassium hydrogen phosphate 2.5g / L, magnesium sulfate heptahydrate 0.5g / L, manganese sulfate monohydrate 0.3g / L, Tween 80 1.5mL / L, Agar 20g / L, pH 6.6; Streak inoculation of L.casei ATCC 393 on MRS solid medium, culture anaerobically at 38°C for 42h;

[0041] Step 2, biosynthesis of zinc hydroxide:

[0042] Cultivate the single colony of Lactobacillus casei L.casei ATCC 393 picked with MRS liquid medium, the formula of MRS liquid medium is as follows: peptone 12g / L, beef extract 12g / L, yeast extract 6g / L, dihydrogen citrate Ammonium 2.5g / L, glucose 25g / L, crystalline sodium acetate 6g / L, dipotassium hydrogen phosphate 2.5g / L, magnesium sulfate heptahydrate 0.6g / L, manganes...

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Abstract

The invention discloses a method for preparing nanometer zinc oxide based on Lactobacillus casei secretion. The method is used for solving the technical problem of low purity of nanometer zinc oxide prepared by existing methods. The method comprises the following steps: preparing an intermediate Zn(OH)2 by using anhydrous zinc acetate as a substrate and using Lactobacillus casei L. Casei ATCC 393as a biological medium, magnetically stirring the intermediate Zn(OH)2 at a constant temperature, and then calcining the stirred intermediate at a high temperature to obtain nano-ZnO. Compared with the background art, the method in the invention has the following advantages: the anhydrous zinc acetate is less toxic than ZnCl2; the Lactobacillus casei L. Casei ATCC 393 is safer than Lactobacillus saliva L3; and the preparation of nano-ZnO is based on the fermentation product of the L. casei ATCC 393, rather than the bacterium itself, so the separation and purification process is simplified, andthe yield and purity of the prepared product nano-ZnO are high.

Description

technical field [0001] The invention relates to a method for preparing nano zinc oxide, in particular to a method for preparing nano zinc oxide based on the secretion of Lactobacillus casei. Background technique [0002] The document "Studies on the Biosynthesis of Nano-zinc Oxide by Lactobacillus salivarius L3" Modern Food Science and Technology, No. 9, 2013, Page 2192-2198" discloses a preparation method of nano-zinc oxide. The biological medium used in this method is from weaning Lactobacillus salivarius L3 isolated from piglet feces. The preparation process is to adjust the pH of the culture medium after the fermentation of Lactobacillus salivarius L3 is completed, and add an appropriate concentration of ZnCl 2 The solution was incubated in a water bath for a certain period of time, and aged at 37°C for 12 hours. The product was collected by centrifugation, washed three times, and dried to constant weight, which was nano-ZnO. However, the ZnCl used in this production pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01G9/02B82Y30/00C12N1/20C12P3/00C12R1/245
CPCB82Y30/00C01G9/02C12N1/20C12P3/00
Inventor 徐春兰豆喜娜乔磊马丽闫述琦张宝华
Owner NORTHWESTERN POLYTECHNICAL UNIV
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