Bifidobacterium pseudosmall chain ccfm1047, its composition, fermented food, use, bacterial agent and preparation method thereof

A technology of bifidobacteria and fermented food, applied in the field of microorganisms, can solve the problems of stimulating the gastrointestinal tract, liver function damage, affecting patients, etc., and achieve the effects of relieving weight gain, relieving metabolic syndrome, and improving content increase

Active Publication Date: 2022-05-03
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These drugs have a good curative effect on each single risk factor of metabolic syndrome. However, long-term use of drugs has certain side effects. For example, long-term use of metformin can cause irritation to the gastrointestinal tract of some patients and cause discomfort and may affect the patient’s response to vitamin B12. The absorption of rosiglitazone can cause liver function damage and edema, etc.

Method used

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  • Bifidobacterium pseudosmall chain ccfm1047, its composition, fermented food, use, bacterial agent and preparation method thereof
  • Bifidobacterium pseudosmall chain ccfm1047, its composition, fermented food, use, bacterial agent and preparation method thereof
  • Bifidobacterium pseudosmall chain ccfm1047, its composition, fermented food, use, bacterial agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] Example 1: Pseudo-small chain Bifidobacterium CCFM1047 has good tolerance to simulated gastrointestinal fluid

[0085] Inoculate the cryopreserved Bifidobacterium CCFM1047 in mMRS medium (MRS medium + 0.05% cysteine ​​hydrochloride), culture anaerobically at 37°C for 48 hours, and then subculture in mMRS medium for 2 After ~3 times, take 1mL of the culture solution of Bifidobacterium pseudospermia CCFM1047, mix it with 9.0mL of pH 2.5 artificial simulated gastric juice (mMRS medium containing 1% pepsin, pH=2.5), and anaerobically For culture, samples were taken at 0h, 0.5h, 1h and 2h respectively, poured and cultured with mMRS agar medium to count plate colonies, determine the number of viable bacteria and calculate their survival rate.

[0086] The survival rate is the ratio of the logarithmic value of the number of viable bacteria at the time of sampling to the logarithmic value of the number of viable bacteria at the 0th hour in the culture solution, expressed in %. ...

Embodiment 2

[0091] Example 2: Bifidobacterium pseudosmall-chain CCFM1047 has no toxic or side effects on C57BL / 6J mice Bifidobacterium pseudo-small-chain CCFM1047 was resuspended in 3% sucrose solution to make a concentration of 3.0×10 8 CFU / mL bacterial suspension. Six healthy male C57BL / 6J mice with a body weight of about 20 g were taken. After one week of adaptation to the environment, the bacterial suspension of this concentration was administered orally once a day, observed for one week, and the death and body weight were recorded.

[0092] The results of these tests are listed in Table 3. These results indicate that feeding concentrations of 3.0×10 8 The CFU / mL pseudosmall chain Bifidobacterium CCFM1047 had no significant impact on mice, no significant change in body weight, and no death. The appearance of the mice had no obvious pathological symptoms.

[0093] Table 3 Changes in body weight and death of mice

[0094]

[0095] Note: -: no death of mice

Embodiment 3

[0096] Example 3: Pseudomonas bifidobacterium CCFM1047 has a recovery effect on the intestinal flora imbalance caused by high-fat feed

[0097] 36 healthy male C57BL / 6J mice weighing 18-20 g were taken, adapted to the environment for 1 week, and randomly divided into 6 groups: blank control group (NC), high-fat model control group (HFD), simvastatin control group (SC ), rosiglitazone control group (RH), Bifidobacterium pseudospermia CCFM1047 intervention group (CCFM1047), Bifidobacterium animalis BB12 control group (BB12), each group contained 6 mice, and the bacteria suspension The dose is 3.0 x 10 8 CFU / mL, resuspended in 3% sucrose solution. The grouping and treatment methods of experimental animals are shown in Table 4:

[0098] Table 4 Grouping of experimental animals

[0099]

[0100] At the end of the experiment, the fresh feces of the mice were collected and frozen at -80°C, the metagenome in the feces was extracted, and the structure of the intestinal flora was ...

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Abstract

The present invention discloses bifidobacterium pseudosmall chain CCFM1047, its composition, fermented food, application, bacterial agent and preparation method thereof. Bifidobacterium pseudosmall chain CCFM1047 can tolerate human gastrointestinal environment and significantly improve metabolic syndrome Fasting blood glucose and oral glucose tolerance in mice, reduce the area under the curve of glucose tolerance drugs, reduce serum insulin levels; significantly alleviate the weight gain of mice; significantly improve the low density of serum in mice with metabolic syndrome caused by high-fat diet Elevated lipoprotein cholesterol and liver triglyceride content; significantly alleviated the increase of Proteus genus in the intestine caused by high-fat diet, pseudosmall chain Bifidobacterium CCFM1047 was used to prepare and relieve metabolic syndrome, reduce Parkinson's, intestinal infection and The pharmaceutical composition and fermented food for urinary tract, kidney infection and other diseases have very broad application prospects.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to pseudosmall chain bifidobacterium CCFM1047, its composition, fermented food, application, bacterial agent and a preparation method thereof. Background technique [0002] In recent years, with the continuous development of our country's social and economic level, the living standards of the public have continued to improve, the proportion of carbohydrates and fats in the diet structure has increased day by day, and the restrictions of many working conditions have made it easy for people to develop sedentary behavior Habit. These external environmental factors combined with their own genetic factors have led to the continuous increase in the incidence of metabolic syndrome in the Chinese population. According to reports, between 2002 and 2010, the proportion of people over the age of 20 in my country with metabolic syndrome rose from 5.4% to 21.3%, an increase of 4 ti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61K35/745A61P3/06A61P3/10A61P1/16A61P25/16A61P31/00A23C9/123A23C13/16A23C19/032A23L11/50A23L11/60A23L11/65A23L33/00C12R1/01
CPCC12N1/20A61K35/745A61P3/06A61P3/10A61P1/16A61P25/16A61P31/00A23C9/1234A23C13/16A23C19/0323A23C11/106A23L33/00A61K2035/115A23V2002/00A23L11/50C12R2001/01C12N1/205A23V2400/535A23V2200/3204A23V2200/3262A23V2200/328A23V2200/32A23V2200/322A23V2200/30
Inventor 陈卫王刚刘煜飞赵建新张灏
Owner JIANGNAN UNIV
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