Embedding medium for biological tissue embedding and embedding method

Abstract

The invention discloses an embedding method for treating a biological tissue and an embedding medium. A soft embedding material is constructed by gel, an auxiliary component is added, and the embedding material and an embedded tissue are made to have approximate expansion characteristics in tissue transparency treatment. The biological tissue treated by using the embedding method and the embeddingmedium can acquire thick slices, the same expansion characteristics with the tissue during the tissue transparency treatment process can be achieved, a sample is protected and restrained during tightbinding between the embedding material and the biological tissue, tissue slicing, transparency and imaging, and the integrity of the sample morphology and the content can be ensured.

Description

technical field [0001] The invention belongs to the technical field of biological histology, and in particular relates to an embedding agent and an embedding method for biological tissue embedding. Background technique [0002] An important direction of biomedical microscopic imaging is high-resolution three-dimensional fluorescence microscopic imaging of large tissue samples, complete tissues and organs, and even the organism itself. Traditionally, tissue transparent imaging and tissue sectioning, slice imaging followed by reconstruction are the main methods for 3D imaging of such large samples. However, tissue transparency of large samples is very time-consuming and has low resolution; traditional slice reconstruction methods are limited to imaging depth and can only use thinner slices. Large sample imaging requires many tissue slices, which is also very time-consuming and brings Massive tissue loss. [0003] In order to meet the requirements of high-throughput three-dim...

AI Technical Summary

Problems solved by technology

The traditional embedding method using hard materials such as resin or paraffin is not easy to obtain thick slices, and at the same time, it has a different expansion coefficient from the tissue during the tissue clearing process, which will cause serious deformation and is incompatible with the tissue clearing; use agarose The embedding of other materials is easy to crack and break during the tissue transparent process, resulting in the loss of tissue on the sample surface

In some cases, when the section contains multiple pieces of isolated tissue, the cracking and fragmentation of the embedding material may even cause the loss of large pieces of tissue, seriously affecting the quality and integrity of imaging

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