A kind of embedding agent and embedding method for biological tissue embedding
An embedding agent and tissue sample technology, applied in the field of biological histology, can solve the problems of easy cracking and breaking, affecting imaging quality and integrity, cracking and breaking of embedding materials, etc., to achieve the effect of ensuring integrity
Active Publication Date: 2020-08-25
UNIV OF SCI & TECH OF CHINA
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Problems solved by technology
The traditional embedding method using hard materials such as resin or paraffin is not easy to obtain thick slices, and at the same time, it has a different expansion coefficient from the tissue during the tissue clearing process, which will cause serious deformation and is incompatible with the tissue clearing; use agarose The embedding of other materials is easy to crack and break during the tissue transparent process, resulting in the loss of tissue on the sample surface
In some cases, when the section contains multiple pieces of isolated tissue, the cracking and fragmentation of the embedding material may even cause the loss of large pieces of tissue, seriously affecting the quality and integrity of imaging
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[0028] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with specific embodiments and with reference to the accompanying drawings.
[0029] The core purpose of the present invention is to construct a soft embedding, so that the embedded biological tissue can be cut into thick slices, and no serious deformation and fragmentation will occur during the transparentization process of the thick slices. Around this purpose, the present invention uses gel as the embedding, adding a gel performance regulator to make the embedding have expansion properties and hardness similar to that of biological tissue during the transparent treatment process, and can also add a fixative to improve embedding. The binding strength between the object and the embedded tissue. Wherein the gel is preferably a long-chain gel, and the gel is formed from a gel precursor composition, ...
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The invention discloses an embedding method for treating a biological tissue and an embedding medium. A soft embedding material is constructed by gel, an auxiliary component is added, and the embedding material and an embedded tissue are made to have approximate expansion characteristics in tissue transparency treatment. The biological tissue treated by using the embedding method and the embeddingmedium can acquire thick slices, the same expansion characteristics with the tissue during the tissue transparency treatment process can be achieved, a sample is protected and restrained during tightbinding between the embedding material and the biological tissue, tissue slicing, transparency and imaging, and the integrity of the sample morphology and the content can be ensured.
Description
technical field [0001] The invention belongs to the technical field of biological histology, and in particular relates to an embedding agent and an embedding method for biological tissue embedding. Background technique [0002] An important direction of biomedical microscopic imaging is high-resolution three-dimensional fluorescence microscopic imaging of large tissue samples, complete tissues and organs, and even the organism itself. Traditionally, tissue transparent imaging and tissue sectioning, slice imaging followed by reconstruction are the main methods for 3D imaging of such large samples. However, tissue transparency of large samples is very time-consuming and has low resolution; traditional slice reconstruction methods are limited to imaging depth and can only use thinner slices. Large sample imaging requires many tissue slices, which is also very time-consuming and brings Massive tissue loss. [0003] In order to meet the requirements of high-throughput three-dim...
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IPC IPC(8): G01N1/30
CPCG01N1/30
Inventor 王浩毕国强祝清源刘北明
Owner UNIV OF SCI & TECH OF CHINA