A kind of isolated windproof polysaccharide and its application
A polysaccharide and glucose technology, applied in the field of medicine, can solve problems such as diabetes and hyperlipidemia that have not been reported yet
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Embodiment 1
[0081] Embodiment 1: the preparation of windproof polysaccharide
[0082] (1) Add 9 L of 0.1 mol / L NaOH aqueous solution to 300 g of Fangfeng decoction pieces. The Radix Fangfeng decoction pieces were extracted with the NaOH aqueous solution at 90° C. for 3 hours to obtain an alkaline extract. After separating the alkaline extract, repeat the extraction twice, each time using 9L 0.1mol / L NaOH aqueous solution, each time for 3 hours. Combine the resulting alkaline extracts.
[0083] (2) adding hydrochloric acid to the alkaline extract to adjust the pH to 7 to obtain a neutral extract, and concentrating the neutral extract to 2L.
[0084] (3) adding ethanol to the neutral extract to obtain a mixture with an ethanol concentration of 20%, and centrifuging the mixture to obtain a supernatant.
[0085] (4) adding ethanol to the supernatant to obtain a mixture with an ethanol concentration of 80%, and centrifuging the mixture to obtain a precipitate;
[0086] (4') Add 0.5 L of di...
Embodiment 2
[0088] Embodiment 2: the preparation of windproof polysaccharide
[0089] (1) Add 9L of 0.05mol / L KOH aqueous solution to 300g Fangfeng decoction pieces. Extract the Radix decoction pieces with the KOH aqueous solution at 40° C. for 4 hours to obtain an alkaline extract. After separating the alkaline extract, the extraction was repeated 3 times, using 9 L of 0.05 mol / L KOH aqueous solution for 4 hours each time. Combine the resulting alkaline extracts.
[0090] (2) Add formic acid to the alkaline extract to adjust the pH to 7 to obtain a neutral extract, which is concentrated to 2L.
[0091] (3) Methanol was added to the neutral extract to obtain a mixture with a methanol concentration of 30%, and the mixture was centrifuged to obtain a supernatant.
[0092] (4) adding methanol to the supernatant to obtain a mixture with a methanol concentration of 90%, and centrifuging the mixture to obtain a precipitate;
[0093] (4') Add 0.5 L of distilled water to the precipitate to di...
Embodiment 3
[0095] Embodiment 3: the preparation of windproof polysaccharide
[0096] (1) Add 5mol / L of Na to 300g Fangfeng decoction pieces 2 CO 3 Aqueous solution 3L. at 90°C with the Na 2 CO 3 Extract the Fangfeng decoction pieces with an aqueous solution for 1 hour to obtain an alkaline extract. After separating the alkaline extract, repeat the extraction 3 times, each using 3L5mol / L of Na 2 CO 3 Aqueous solution, 1 hour each time. Combine the resulting alkaline extracts.
[0097] (2) Add acetic acid to the alkaline extract to adjust the pH to 7 to obtain a neutral extract, and concentrate the neutral extract to 2L.
[0098](3) Add ethanol to the neutral extract to obtain a mixture with an ethanol concentration of 15%, and centrifuge the mixture to obtain a supernatant.
[0099] (4) adding ethanol to the supernatant to obtain a mixture with an ethanol concentration of 80%, and centrifuging the mixture to obtain a precipitate;
[0100] (4') Add 0.5 L of distilled water to the...
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