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Cultivation method and application of bonsai ornamental rice

A cultivation method and bonsai technology, applied in the field of landscape rice breeding and molecular genetics, to achieve the effect of speeding up the breeding process, deepening understanding and improving breeding efficiency

Inactive Publication Date: 2019-08-16
江西省农业科学院水稻研究所
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Based on the existing technology, the current breeding work of ornamental rice mainly focuses on rice leaf color and rice flower shape, and the use of rice dwarf cluster mutation materials to obtain dwarf cluster bonsai ornamental rice varieties through selection has not yet been seen. to report

Method used

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  • Cultivation method and application of bonsai ornamental rice
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  • Cultivation method and application of bonsai ornamental rice

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Experimental program
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Effect test

Embodiment 1

[0028] Embodiment 1: take D27 gene dwarf cluster mutant as donor parent to cultivate dwarf cluster bonsai ornamental rice

[0029] Such as figure 1 As shown in the breeding process, the specific breeding process is:

[0030] (1) F1 was obtained by crossing the dwarf bushy mutant of D27 gene with the recipient parent Huang Huazhan.

[0031] (2) D27 gene mutants and Huanghuazhan F1 generation continued to backcross with the recipient parent Huanghuazhan, DNA was extracted from a single plant at the seedling stage of BC1F1 generation, and the SSR molecular marker RM206 (F-CCCATGCGTTTAACTATTCT; R-CGTTCCATCGATCCGTATGG) linked to the D27 gene was used to conduct detection, such as figure 2 Genotype analysis was carried out as indicated, and the seedlings with the D27 mutant gene were reserved for planting selection. Individual plants carrying the D27 mutant gene with satisfactory agronomic traits were selected for the next round of backcrossing and testing until the main traits ...

Embodiment 2

[0033] Embodiment 2: take D61 gene dwarf cluster mutant as donor parent to cultivate dwarf cluster bonsai ornamental rice

[0034] Such as figure 1 As shown in the breeding process, the specific breeding process is:

[0035] (1) The D61 gene dwarf bushy mutant was crossed with the recipient parent Nanfangjing 1 to obtain F1.

[0036] (2) The D61 gene mutant and the F1 generation of Nanfangjing 1 continued to be backcrossed with the recipient parent Nanfangjing 1, DNA was extracted from a single plant at the seedling stage of the BC1F1 generation, and the SSR molecular marker RM128 (F-AGCTTGGGTGATTTCTTGGAAGCG) linked to the D61 gene was used ;

[0037] R-ACGACGAGGAGTCGCCGTGCAG) for detection, such as image 3 Genotype analysis was performed as indicated, and the seedlings with the D61 mutant gene were reserved for planting selection. Individual plants carrying the D61 mutant gene with satisfactory agronomic traits were selected for the next round of backcrossing and testing...

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Abstract

The invention relates to the technical field of landscape rice breeding and molecular genetics and particularly discloses a cultivation method and an application of bonsai ornamental rice. The cultivation method comprises the steps as follows: a short stalk cluster mutant and a receptor parent are hybridized to obtain F1; the F1 generation is continuously backcrossed with the receptor parent to obtain a BC1F1 generation, and single plant DNA is extracted at the seedling stage of the BC1F1 generation for molecular mark detection; the BC1F1 generation is continuously backcrossed with the receptor parent to obtain a BC2F1 generation, and single plant DNA is extracted at the seedling stage of the BC2F1 generation for molecular mark detection; the BC2F1 generation and the receptor parent are subjected to advanced backcross to obtain a BCnF1 generation, and single plant DNA is extracted at the seedling stage of the BCnF1 generation for molecular mark detection; a bonsai ornamental rice variety is bred from descendant strains of the BCnF1 after advanced backcross. As detection auxiliary selection is performed by mutant gene molecular markers at the seedling stage of lower generations, thebreeding process is accelerated, and the breeding efficiency is improved; the cultivated short stalk cluster bonsai ornamental rice variety can enrich bonsai types, popularize rice knowledge and deepen understanding of Chinese farming culture.

Description

technical field [0001] The invention belongs to the technical field of landscape rice breeding and molecular genetics, and in particular relates to a cultivation method and application of bonsai ornamental rice. Background technique [0002] China is one of the countries with the earliest agricultural development in the world. The farming culture has a long history. It not only provided material living materials for hundreds of millions of people in the past, but also created favorable conditions for the development of science and culture. Rice is one of the most important food crops in my country, the planting area accounts for 30% of the food crop area, and it is the staple food of more than 60% of the population in my country. Rice planting has a long history. About 7,000 years ago, there were rice-growing agricultural settlements in Hemudu, Yuyao, Zhejiang Province. In places such as Pengtou Mountain in Li County, Yuchan Rock in Dao County, Wannian Xianren Cave and Diaot...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01G22/22C12Q1/6895
CPCA01H1/02A01G22/22C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 陈明亮沈雨民肖叶青熊焕金吴小燕邓伟
Owner 江西省农业科学院水稻研究所
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