Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of extraction method of polysaccharide of small molecule dendrobium officinale

A Dendrobium officinale, small molecule technology, applied in the direction of allergic diseases, drug combination, etc., can solve problems such as unclear mechanism, and achieve the effect of improving immune function, good activity, and good immune promotion effect

Active Publication Date: 2020-01-07
浙江寿而健健康产品有限公司
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the mechanism is not clear, and it is speculated that the local high temperature and high pressure environment generated by ultrasound hydrolyzes polysaccharides.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] In step (1), take 100 g of shade-dried Dendrobium officinale powder, add 400 mL of petroleum ether, heat and reflux for defatting for 1 hour, and repeat 3 times to obtain defatted Dendrobium officinale powder. Petroleum ether can be recycled after distillation.

[0040] In step (2), add 400 mL of 70% ethanol to the degreased dendrobium powder obtained in step (1) and extract for 8 hours* twice, filter, and the filtrate can be used to extract dendrobium alkaloids, and the solid phase is the filter residue.

[0041] Step (3): the first water extraction. Add 500mL of water to the filter residue obtained in step (2), heat to 60°C, extract for 2h*3 times, and filter to obtain the combined first water extract and first water extract filter residue. The first aqueous extract was concentrated to 500 mL.

[0042]Step (4): rough separation. A 50kD (50,000 Dalton) ultrafiltration membrane is used to intercept macromolecular polysaccharides in the concentrate. Add 3 times the v...

Embodiment 2

[0046] In step (1), take 100 g of shade-dried Dendrobium officinale powder, add 400 mL of petroleum ether, heat and reflux for defatting for 1 hour, and repeat 3 times to obtain defatted Dendrobium officinale powder. Petroleum ether can be recycled after distillation.

[0047] In step (2), add 400 mL of 80% ethanol to the degreased dendrobium powder obtained in step (1) and extract for 10 h twice, filter, and the filtrate can be used to extract dendrobium alkaloids, and the solid phase is the filter residue.

[0048] Step (3): the first water extraction. Add 600mL of water to the filter residue obtained in step (2), heat to 70°C, extract for 3h*2 times, and filter to obtain the combined first water extract and the first water extract filter residue. The first aqueous extract was concentrated to 500 mL.

[0049] Step (4): rough separation. A 50kD (50,000 Dalton) ultrafiltration membrane is used to intercept macromolecular polysaccharides in the concentrate. Add 3 times the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an extraction method of macromolecular dendrobium officinale polysaccharide, and relates to the field of extraction of dendrobium officinale. The extraction method of the macromolecular dendrobium officinale polysaccharide comprises the steps of firstly, conducting water extraction to obtain dendrobium officinale polysaccharide, and cutting off macromolecular polysaccharide; conducting acid extraction on the macromolecular polysaccharide and water extraction residues to obtain micromolecular polysaccharide, so as to improve the extraction efficiency of polysaccharide; and then conducting washing to remove monosaccharide and oligosaccharide with a molecule weight below 2 kD, so as to obtain a product with a molecule weight ranging from 2 kD to 50 kD basically. Polysaccharide is subjected to deep processing, the comprehensive utilization rate of polysaccharide is improved, and especially, the yield of micromolecular polysaccharide is improved. A biological experiment proves that hydrolyzed polysaccharide also has good activity in the aspect of improving the immune function.

Description

technical field [0001] The invention relates to the field of extraction of dendrobium candidum, more specifically to an extraction process of dendrobium polysaccharides in dendrobium candidum, and in particular to a process for increasing the extraction rate of polysaccharides in dendrobium candidum by using acid hydrolysis. Background technique [0002] The medicinal use of Dendrobium was first recorded in "Shen Nong's Materia Medica" before 2000 and "Famous Doctors" in the Eastern Han Dynasty. Many ancient and modern famous teachers and classic prescriptions have Dendrobium. The common ones in my country are Dendrobium nobile, Dendrobium officinale, Dendrobium cherry, Dendrobium bark, Dendrobium sheath, Dendrobium slender stem, Dendrobium spring and so on. [0003] Dendrobium officinale Kimura.et Migo is a perennial herb of the genus Dendrobium orchidaceae. Its main medicinal parts are fresh or dried stems. It is mainly distributed in Anhui, Zhejiang, Guangxi, Hunan, Yunna...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61P37/04
CPCA61P37/04C08B37/0003
Inventor 谢恬邱君焕
Owner 浙江寿而健健康产品有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com