Fluorescence detection device for DNA (deoxyribonucleic acid) repeat sequences

Abstract

The invention discloses a fluorescence detection device for DNA (deoxyribonucleic acid) repeat sequences. The fluorescence detection device comprises a sliding chute frame, a reaction groove, an electromagnetic wave emission device, an electromagnetic wave receiving device, an information processing and displaying device and two water channels; each water channel is provided with a heating unit and a temperature control unit, a propeller is arranged in the water channel, the water channels are communicated with the reaction groove, the electromagnetic wave emission device is located directly above the reaction groove, the electromagnetic wave receiving device is located directly blow the reaction groove, the electromagnetic wave receiving device is aligned with the electromagnetic wave emission device, the information processing and displaying device is connected with the electromagnetic wave receiving device, the emission wavelength of the electromagnetic wave emission device is 492nm, and the receiving wave of the electromagnetic wave receiving device is 518nm. By the arrangement, the relative length of the DNA repeat sequences can be acquired by detecting the fluorescence valueaccurately and rapidly.

Description

technical field [0001] The invention relates to the field of medical equipment, in particular to a fluorescence detection device for DNA repeat sequences. Background technique [0002] DNA repeat sequence (repeat sequence) refers to the multiple copies of the gene sequence, which can be divided into three categories according to the frequency of DNA repetition: 1. Highly repetitive sequence: DNA repeats hundreds to millions of times, generally less than 10 nuclei Short stretches of nucleotide residues. Such as satellite DNA on heterochromatin. They are fragments that are not translated. In mice, approximately 10% of the genome is folded with moderately repeated sequences. 2. Moderately repetitive sequence: DNA repeats from ten to several hundred times. About 20% in mice. Such as rRNA genes, tRNA genes and genes of certain proteins (such as histone, actin, keratin, etc.). 3. Single-copy sequence: a sequence that is unique in the entire genome and has no similar repeats....

AI Technical Summary

Problems solved by technology

The telomere sequence measured by this technique also includes the sequence of the sub-telomeric region, which cannot provide the actual length of the telomere, nor can it accurately quantify the length of the telomere

At the same time, this technique is time-consuming and labor-intensive, and requires a large amount of DNA

[0006] 2. flow-FISH, which combines flow cytometry and fluorescence in situ hybridization, but the technology is complicated, and there are inherent contradictions between cell fixation and complete denaturation of chromosomal DNA, and the number of hybridized probes entering cells is related to the number of unhybridized probes washed out The integrity of the cells cannot be controlled, and the cells need to be kept intact throughout the experimental process for analysis, and the quantification is not accurate enough

[0013] At present, the existing technology, such as a biological chromosome telomere DNA length detection device (application number: 201720631804.0), although it can complete the temperature control process through a water bath without manual operation, the temperature change process is not fast enough. The structure of the arm and other parts is complex and expensive. More importantly, the utility model does not include fluorescence instrument equipment, and because there is no data processing equipment, it is impossible to directly obtain the accurate length of the telomere DNA of the biological chromosome from the utility model provided by the prior art.

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