Method for creating plant male sterile line

Abstract

The invention discloses a method for creating a plant male sterile line. The method includes: lowering content of protein shown as a sequence 3 in a receiver plant or inhibiting activity of the protein in the receiver plant or knocking out coding gene of the protein in the receiver plant or reducing expression of the coding gene of the protein in the receiver plant to obtain the plant sterile line. Fertility of the plant sterile line obtained by the method can be restored by applying jasmonate or derivative thereof, and the method is not limited by genetic background, avoids negative effect ofsterile cytoplasm, is of important significance in expanding plant sterile line source, simplifying breeding process, accelerating plant breeding speed and lowering breeding cost and can promote quick development of different plant crossbreeding.

Description

technical field [0001] The invention relates to a method for creating male sterile lines of plants in the field of biotechnology. Background technique [0002] Hybrid breeding is an important means of creating variation and utilizing heterosis, and is one of the most effective breeding methods. Male sterility is the basis and effective tool of hybrid breeding, and has important scientific and industrial value. Male sterile plants refer to the phenomenon that the male reproductive organs of plants cannot produce normally functioning pollen. At present, male sterile lines used for plant breeding are often found from natural populations, with limited species, long breeding cycle, low efficiency and high price. [0003] The development of rice hybrid breeding is the most mature. In the cultivation of three-line hybrid rice, in addition to the sterile line, restorer lines and maintainer lines are also required. Its advantage is that the fertility of the sterile line is stable,...

AI Technical Summary

Problems solved by technology

This method greatly improves the breeding efficiency, and it is less difficult to breed light-temperature-sensitive male sterile lines, but the fertility of light-sensitive and temperature-sensitive lines is easily affected by weather conditions

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