Method for creating plant male sterile line

A plant breeding and plant technology, which is applied in the field of creating male sterile lines of plants, can solve problems such as fertility being easily affected by weather conditions, and achieve the effects of speeding up plant breeding, expanding the source of plant sterile lines, and avoiding negative effects

Active Publication Date: 2019-08-16
TSINGHUA UNIV
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AI Technical Summary

Problems solved by technology

This method greatly improves the breeding efficiency, and it is less difficult to breed light-temperature-sensitive male s

Method used

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  • Method for creating plant male sterile line
  • Method for creating plant male sterile line
  • Method for creating plant male sterile line

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1, creation of rice male sterile line

[0059] In this example, Crispr / Cas9 was used to knock out the OsOPR7 gene in rice, and a male sterile line of rice was obtained. The male sterile line could restore its partial fertility after spraying methyl jasmonate. The sequence is sequence 1 in the sequence listing, its CDS sequence is sequence 2 in the sequence listing, and encodes the OsOPR7 protein shown in sequence 3 in the sequence listing. The specific operation steps are as follows:

[0060] 1. Construction of Crispr / Cas9 knockout vector

[0061] The Crispr / Cas9-OsOPR7 vector used to knock out the OsOPR7 gene in rice was constructed, and the selected target sequence was: 5'-GAAGGTTTCCTCATGTCCCT-3' (position 1207-1226 of Sequence 1 in the sequence listing). The steps to construct the Crispr / Cas9-OsOPR7 vector are as follows:

[0062] A. Construction of OsSg-OPR7-T1 plasmid: 1) Design and synthesize the primer sequence of OPR7-T1, each taking an equal amoun...

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Abstract

The invention discloses a method for creating a plant male sterile line. The method includes: lowering content of protein shown as a sequence 3 in a receiver plant or inhibiting activity of the protein in the receiver plant or knocking out coding gene of the protein in the receiver plant or reducing expression of the coding gene of the protein in the receiver plant to obtain the plant sterile line. Fertility of the plant sterile line obtained by the method can be restored by applying jasmonate or derivative thereof, and the method is not limited by genetic background, avoids negative effect ofsterile cytoplasm, is of important significance in expanding plant sterile line source, simplifying breeding process, accelerating plant breeding speed and lowering breeding cost and can promote quick development of different plant crossbreeding.

Description

technical field [0001] The invention relates to a method for creating male sterile lines of plants in the field of biotechnology. Background technique [0002] Hybrid breeding is an important means of creating variation and utilizing heterosis, and is one of the most effective breeding methods. Male sterility is the basis and effective tool of hybrid breeding, and has important scientific and industrial value. Male sterile plants refer to the phenomenon that the male reproductive organs of plants cannot produce normally functioning pollen. At present, male sterile lines used for plant breeding are often found from natural populations, with limited species, long breeding cycle, low efficiency and high price. [0003] The development of rice hybrid breeding is the most mature. In the cultivation of three-line hybrid rice, in addition to the sterile line, restorer lines and maintainer lines are also required. Its advantage is that the fertility of the sterile line is stable,...

Claims

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Application Information

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IPC IPC(8): C12N15/82C12N15/29C07K14/415A01H5/00A01H6/20A01H6/46
CPCC07K14/415C12N15/8213C12N15/8289
Inventor 谢道昕闫建斌鄢纯张晓林怡荣
Owner TSINGHUA UNIV
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