Semaphorin7A monoclonal antibody and application thereof in preparing drugs for treating inflammatory disease
A monoclonal antibody, inflammatory disease technology, applied in the direction of resisting vector-borne diseases, anti-animal/human immunoglobulins, antibodies, etc., to achieve obvious curative effects
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Embodiment 1
[0057] This example provides two Semaphorin7A monoclonal antibodies: 2567-1 and 2567-3, both of which include heavy chain variable regions and light chain variable regions.
[0058] Among them, the heavy chain variable region of Semaphorin7A monoclonal antibody 2567-1 includes the following 3 CDR amino acid sequences and a nucleotide sequence: the amino acid sequence is VH CDR1 of SEQ ID NO.2, and the amino acid sequence is SEQ ID NO.3. VHCDR2, the amino acid sequence is VH CDR3 of SEQ ID NO.4, and the nucleotide sequence is the nucleotide of SEQ ID NO.5. The light chain variable region of Semaphorin7A monoclonal antibody 2567-1 includes the following 3 CDR amino acid sequences and a nucleotide sequence: the amino acid sequence is VL CDR1 of SEQ ID NO.6, and the amino acid sequence is VL CDR2 of SEQ ID NO.7 , the amino acid sequence is the VL CDR3 of SEQ ID NO.8, and the nucleotide sequence is the nucleotide of SEQ ID NO.9.
[0059] Similarly, the heavy chain variable region ...
Embodiment 2
[0079] This example provides the application of a Semaphorin7A monoclonal antibody (2567-1, 2567-3) in the treatment of viral myocardial inflammation induced by CVB3.
[0080] First construct the viral myocarditis model induced by CVB3; the construction steps of the model specifically include:
[0081] (1) Get 6-8 week-old male Balb / c mice, divide them into 4 groups, 6 in each group, and give each mouse 10 3 CVB3 virus at a dose of TCID50 induces viral myocardial inflammation in mice.
[0082] (2) On the first day, third day and fifth day of virus infection, 50ug / mouse of Sema7A2567-1 or 2567-3 antibody was given to mice by intraperitoneal injection respectively, and the CVB3 group was the non-treatment control group for virus infection, control group of healthy mice.
[0083] like Figure 6 As shown, on the 7th day of infection, the myocarditis index observed in mice was compared with that in the control group. After treatment with the mouse Sema7A antibody 2567-1, the ser...
Embodiment 3
[0092] This example provides the application of a Semaphorin7A monoclonal antibody (2567-1, 2567-3) in the treatment of sepsis inflammation induced by Staphylococcus aureus (S.aureus).
[0093] Similarly, construct the septic blood inflammation model induced by Staphylococcus aureus (S.aureus); the construction steps of the model specifically include:
[0094] (1) Get 6-8 week-old male C57bl / 6 mice, divide them into 4 groups, 6 mice in each group, and give each mouse 10 7 Staphylococcus aureus at a dose of CFU0 induces an inflammatory model of septic blood mice.
[0095] (2) On the first day, the third day and the fifth day of bacterial infection, mice were given Sema7A 2567-1 or 2567-3 antibody 50ug / mouse by intraperitoneal injection, and the S.aureus group was the bacterial infection non-treatment control group, control is the healthy mouse group.
[0096] The inflammatory cytokines in the serum of infected mice on the 6th day were detected, and the detection results were ...
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