Method for detecting microorganism content in complex solution based on ultraviolet-visible spectrum

Abstract

The invention provides a method for detecting the microorganism content in a complex solution based on an ultraviolet-visible spectrum, for effectively solving the problems of red shift of a characteristic spectrum peak of a microorganism in the spectrum caused by complex detection background components, overlap of the spectrum peaks between a medium component and the microorganism, and spectral noise interference and the like. According to the method provided by the invention, cow milk is used as a detection background, the Escherichia coli is used as a detection object, and the method comprises the steps of: firstly performing activation, separation and purification on Escherichia coli powder to obtain bacterial suspension of Escherichia coli; obtaining the ultraviolet-visible spectra ofbacterial suspension to be detected with different concentrations in the cow milk by using an ultraviolet-visible spectrophotometer; eliminating baseline drift, spectral noise interference and otherproblems caused by the scattering of macromolecules such as protein, fat and the like in the cow milk by using standard normal variable transformation; separating an overlapping spectrum of the cow milk and the Escherichia coli by using a two-dimensional correlation analysis method; and establishing a prediction model based on an RBF neural network to realize the quantitative detection of the total number of the Escherichia coli in the cow milk.

Description

Technical field [0001] The invention relates to an ultraviolet-visible spectrum detection technology, in particular to a quantitative detection method for the total number of Escherichia coli in milk. Background technique [0002] In recent years, food safety problems caused by food-borne pathogenic microorganisms have frequently occurred. Escherichia coli is the food-borne pathogen with the highest detection rate in cow milk. Therefore, it can capture the specificity of E. coli more accurately and realize its rapid , Efficient testing is of great significance to ensure the safety of dairy products. At present, the main methods for determining the total number of E. coli in milk are: multi-tube fermentation method, plate counting method and biosensor method, but these methods take a long time (48h), are professional in operation, and cannot meet the requirements of rapid , The need for efficient testing. [0003] Ultraviolet-visible spectroscopy is often used to analyze and study...

AI Technical Summary

Problems solved by technology

[0004] However, at present, the quantitative detection of microorganisms generally uses water as the research background and cannot be applied in complex environments. The main reason is that the detection background contains complex components, such as fat, protein and other biological macromolecules in milk due to uneven particle size distribution. Scattering may occur, causing noise interference, resulting in a red shift of the characteristic band of the ultraviolet-visible spectrum of E. coli, making it difficult to pick up, and inaccurate quantitative detection results, etc.

Images