Fluorescent molecularly imprinted polymer hollow microsphere and preparation method and application thereof

A technology of fluorescent molecular imprinting and hollow microspheres, which is applied in the direction of fluorescence/phosphorescence, alkali metal compounds, chemical instruments and methods, etc., can solve the problems of inability to quickly and efficiently detect specificity, unsafe synthesis process, and environmental protection, and achieve fast The effect of efficient synthesis process and shortened detection time

Active Publication Date: 2019-08-30
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The invention provides a fluorescent molecularly imprinted polymer hollow microsphere and its preparation method and application to solve the existing problems that the target analyte cannot be detected quickly and efficiently, and the synthesis process is not safe and environmentally friendly.

Method used

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  • Fluorescent molecularly imprinted polymer hollow microsphere and preparation method and application thereof
  • Fluorescent molecularly imprinted polymer hollow microsphere and preparation method and application thereof
  • Fluorescent molecularly imprinted polymer hollow microsphere and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0049] Include the following steps:

[0050] Step 1: Preparation of silica nanoparticles SiO 2

[0051] First, configure ethanol-deionized water mixed solution, add ammonia water to it, and obtain mixed solution A after ultrasonic treatment for 10 minutes; place mixed solution A at room temperature under magnetic stirring, and slowly add ethyl orthosilicate through a burette under the protection of an inert gas Ester TEOS was reacted until the solution changed from clear and transparent to milky white (obvious change occurred within about 20 minutes), continued to react for 6 hours, collected by centrifugation, washed repeatedly with ethanol-water solution and vacuum freeze-dried for 8 hours to obtain silica Nanoparticles; ammonia water: ethanol: water: tetraethyl orthosilicate is 2:25:25:2, magnetic stirring 600 rpm, centrifugal speed 10000rpm / min, time 10min,

[0052] Step 2: Preparation of silica surface grafted with fluorescein isothiocyanate SiO 2 Polymers by @FITC-APT...

Embodiment 2

[0060] Include the following steps:

[0061] Step 1: Preparation of silica nanoparticles SiO 2

[0062] First, configure ethanol-deionized water mixed solution, add ammonia water to it, and obtain mixed solution A after ultrasonic treatment for 10 minutes; place mixed solution A at room temperature under magnetic stirring, and slowly add ethyl orthosilicate through a burette under the protection of an inert gas Ester TEOS was reacted until the solution changed from clear and transparent to milky white (obvious change occurred within about 20 minutes), continued to react for 6 hours, collected by centrifugation, washed repeatedly with ethanol-water solution and vacuum freeze-dried for 8 hours to obtain silica Nanoparticles; ammonia water: ethanol: water: tetraethyl orthosilicate is 2:25:25:2, magnetic stirring 600 rpm, centrifugal speed 10500rpm / min, 7.5min,

[0063] Step 2: Preparation of silica surface grafted with fluorescein isothiocyanate SiO 2 Polymers by @FITC-APTES; ...

Embodiment 3

[0071] Include the following steps:

[0072] Step 1: Preparation of silica nanoparticles SiO 2

[0073] First, configure ethanol-deionized water mixed solution, add ammonia water to it, and obtain mixed solution A after ultrasonic treatment for 10 minutes; place mixed solution A at room temperature under magnetic stirring, and slowly add ethyl orthosilicate through a burette under the protection of an inert gas Ester TEOS was reacted until the solution changed from clear and transparent to milky white (obvious change occurred within about 20 minutes), continued to react for 6 hours, collected by centrifugation, washed repeatedly with ethanol-water solution and vacuum freeze-dried for 8 hours to obtain silica Nanoparticles; ammonia water: ethanol: water: tetraethyl orthosilicate 2:25:25:2, magnetic stirring 600 rpm, centrifugal speed 11000 rpm / min, 5 min;

[0074] Step 2: Preparation of silica surface grafted with fluorescein isothiocyanate SiO 2 Polymers by @FITC-APTES;

...

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Abstract

The invention relates to a fluorescent molecularly imprinted polymer hollow microsphere, a preparation method and application thereof, and belongs to the technical field of molecularly imprinted polymers. The preparation method comprises the following steps: preparing silicon dioxide nano-particles SiO2, preparing a polymer of silicon dioxide surface grafted fluorescein isothiocyanate SiO2@FITC-APTES; and preparing fluorescent molecular imprinting polymer SiO2@FITC-APTES@MIP, and etching the polymer to obtain hollow fluorescent molecular imprinting polymer FITC-APTES@MIP. The invention has theadvantages that the hollow fluorescent molecularly imprinted polymer which can rapidly and specifically detect a target analyte with high efficiency, can be prepared, and the synthesis process is safe and environment-friendly, specifically, the polymer can specifically adsorb the target analyte and generate fluorescence quenching, and has a remarkable quenching effect within 20 minutes, thereby shortening the detection time.

Description

technical field [0001] The invention belongs to the technical field of molecularly imprinted polymers, in particular to a fluorescent molecularly imprinted polymer hollow microsphere and its preparation method and application. Background technique [0002] In recent years, more and more attention has been paid to the development of functional materials with characteristic domains through chemical synthesis methods, among which Molecularly Imprinted Polymers (MIP) developed by Molecular Imprinting Technique or Technology (MIT) is one of them. A representative biomimetic functional material, because molecularly imprinted polymers have three characteristics of structural predictability, recognition specificity and application universality and stable physical and chemical properties, simple preparation method, low cost and strong catalytic and degradation ability , so it has received extensive attention in the fields of purification and separation, chemistry, biosensing, artific...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J20/26B01J20/28B01J20/30C08F220/06C08F220/56C08F226/06C08F222/14G01N21/64
CPCB01J20/268B01J20/28021B01J20/28057C08F220/06C08F220/56C08F226/06G01N21/6428G01N21/643G01N2021/6432C08F222/102
Inventor 吕绍武徐朝建黄一高海成龚平生姜蒙蒙马海霞
Owner JILIN UNIV
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