Method of quickly identifying whether living citrus are self-compatible

Abstract

A method of quickly identifying whether living citrus are self-compatible comprises the steps of 1, collecting citrus pollen; 2, preparing a citrus pollen germination medium, scattering pollen to themedium, and observing with a microscope whether activity of the pollen is normal; 3, dipping the pollen with normal activity, applying the pollen to stigmas, and covering each flower with a pollination bag; 4, over a certain period of time which is determined according to a citrus species, immobilizing the stigmas in an immobilizing liquid, washing with an ethanol solution, heating by a water bathafter washing to perform softening for a period of time which is determined according to the citrus species, washing the softened stigmas with clear water, and staining with a water-soluble aniline blue solution; and 5, observing germination condition of pollen on the stigmas with a microscope after staining so as to determine whether the citrus are self-compatible. A normalized process to identify whether citrus are self-compatible is provided herein; stigma acquisition time and softening time are provided for different citrus species after pollination; whether citrus plants are self-compatible can be identified efficiently and accurately.

Description

Technical field [0001] The invention relates to a method for identifying citrus self-compatibility, in particular to a method suitable for rapid identification of citrus self-compatibility in vivo. Background technique [0002] Citrus is the largest fruit tree in the world and the largest fruit tree in southern China, which has great industrial value. In citrus production, seedless fruit is a very important characteristic, which comes from self-incompatibility and parthenocarpy. Therefore, the study of self-incompatibility traits of citrus fruits has important value in production practice . [0003] Self-incompatibility is one of the most important mechanisms of angiosperms to prevent self-pollination, fertilization, and maintain species adaptability. This mechanism rejects or accepts pollen from individuals with similar genetic relationships by regulating stigma recognition, and ultimately achieves optimized offspring The purpose of the genotype. Self-incompatibility is genetic...

AI Technical Summary

Problems solved by technology

The traditional aniline blue dyeing method is mostly used in Solanaceae, Rosaceae and other crops, but in citrus species, the sampling time after pollination, the formula of tissue fixative, and the time of tissue softening and dyeing have not been systematically reported.

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