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Construction method and application of animal model of liver injury induced by tripterygium glycosides

A technology of tripterygium wilfordii polyglycosides and a construction method, which are applied in the field of construction of an animal model of tripterygium wilfordii polyglycosides for liver injury to achieve a cost-effective effect

Inactive Publication Date: 2019-09-06
GUANGDONG FOOD & DRUG VOCATIONAL COLLEGE
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the research on the hepatotoxicity of tripterygium wilfordii polyglycosides has become a bottleneck that restricts researchers from exploring the safety of tripterygium wilfordii on which model organisms

Method used

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  • Construction method and application of animal model of liver injury induced by tripterygium glycosides
  • Construction method and application of animal model of liver injury induced by tripterygium glycosides
  • Construction method and application of animal model of liver injury induced by tripterygium glycosides

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Embodiment 1

[0023] 1 Materials and methods

[0024] 1.1 Experimental animals The wild-type AB strain zebrafish was reproduced by natural pair mating. The age is 3d after fertilization (ie, 3-day-old zebrafish). All zebrafish were kept in fish culture water at 28°C. Experimental animal use license number: SYXK (Zhejiang) 2012-0171. The feeding management complies with the requirements of the international AAALAC certification.

[0025] 1.2 Drugs and reagents Dimethyl sulfoxide (DMSO): Sigma, France. Tripterygium glycosides tablets: Zhejiang Deende Pharmaceutical Co., Ltd., Z33020422, stored in a cool cabinet, prepared with DMSO to make a 100mg / mL mother liquor before use, diluted as needed, and prepared for immediate use. Acetaminophen: purchased from Shanghai Aladdin Biochemical Technology Co., Ltd., white powder, batch number I1706064, prepared with DMSO to 800mM before use, and prepared for immediate use. 4% paraformaldehyde: Dingguo Bio, batch number: 814001300. Xylene: Aladdin, ...

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Abstract

The invention discloses a construction method and application of an animal model of liver injury induced by tripterygium glycosides. The experiment shows that the liver tissue structure of zebrafish can be obviously injured when concentrations of the tripterygium glycosides are 42.5, 128 and 383 mu g / mL, and the ALT, AST and ALP content can be increased in the high dose group of the tripterygium glycosides (the concentration of the tripterygium glycosides is 383 mu g / mL), which indicates that the high dose of the tripterygium glycosides has the effect of liver injury. The animal model of the liver injury induced by the tripterygium glycosides is successfully constructed by treating the zebrafish with a tripterygium glycosides solution; the construction method has the advantages of reliability, rapidity, high efficiency, low cost, high cost performance and the like, opens a new way for subsequent researches to systematically study the hepatotoxicity characteristics and mechanism of thetripterygium glycosides, and provides a theoretical basis for screening hepatoprotective drugs and tripterygium glycosides detoxification drugs.

Description

[0001] Technical field: [0002] The invention belongs to the technical field of medicine, and in particular relates to a method for constructing an animal model of tripterygium wilfordii polyglycosides liver injury and its application. [0003] Background technique: [0004] Tripterygium wilfordii polyglycoside is a traditional Chinese medicine in my country. It is widely used clinically and has definite curative effect, but it is prone to liver toxicity, which limits its clinical application. According to existing studies, commonly used rats and mice are not sensitive animals for the study of tripterygium glycosides hepatotoxicity. At present, the research on the hepatotoxicity of tripterygium wilfordii polyglycosides has become a bottleneck that restricts researchers from exploring the safety of tripterygium wilfordii on which model organisms. In order to comprehensively and deeply explore the characteristics and mechanism of tripterygium glycosides hepatotoxicity, and seek...

Claims

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Application Information

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IPC IPC(8): A01K61/10A01K67/027A61K49/00
CPCA01K67/027A01K2207/20A61K49/0008A01K61/10Y02A40/81
Inventor 付晓春蒋平沈小莉
Owner GUANGDONG FOOD & DRUG VOCATIONAL COLLEGE
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