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A kind of trichoderma viride, bacterial agent, biological fertilizer and application

A green Trichoderma, bio-organic fertilizer technology, applied in fertilizers, applications, microorganisms and other directions made of biological waste, can solve problems such as environmental pollution and low utilization rate of chemical fertilizers, improve soil micro-ecological environment, increase germination rate, The effect of good development and application prospects

Active Publication Date: 2020-04-07
BIOLOGY INST OF SHANDONG ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the utilization rate of chemical fertilizers in a single season in my country is only 30%-35%. The utilization rate of chemical fertilizers is low, and it will also cause serious pollution to the environment.

Method used

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  • A kind of trichoderma viride, bacterial agent, biological fertilizer and application
  • A kind of trichoderma viride, bacterial agent, biological fertilizer and application
  • A kind of trichoderma viride, bacterial agent, biological fertilizer and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1.1 Isolation and purification of functional strains:

[0037] (1) Collection of samples: the samples were collected from tobacco fields in Weifang City, Shandong Province. Remove the top layer of floating soil until the soil layer with plant roots is exposed, shovel out the soil together with the root system, seal it in a plastic bag, and bring it back to the laboratory.

[0038] (2) Isolation of bacterial strains: crush the soil sample into a fine powder, place it in a sterilized Erlenmeyer flask, add sterilized water, and vibrate to make a soil suspension; use sterile normal saline for gradient dilution, and take Drop 100 μL onto a PDA plate, apply the plate dilution method evenly, and culture in a 28°C incubator for 2-4 days; select a single colony and transfer it to a PDA plate to grow colonies, and use the single spore separation method to separate and purify , save for later use. Among them, the formula of PDA medium is: potato dipping powder 6g / L, glucose 20g / ...

Embodiment 2

[0044] Embodiment 2: the pure cultivation of Trichoderma viridans Tv-1511 and the collection of aseptic spores

[0045] (1) Trichoderma viride Tv-1511 was inoculated into a culture bottle equipped with mushroom dregs, and placed in an incubator at 26° C. for 20 days;

[0046] (2) Add sterile water to suspend the spores, pour them into a gauze-sealed triangular flask, remove impurities and mycelium, and collect the spore liquid;

[0047] (3) Centrifuge the spore liquid after balance, 10,000 rpm, 20°C for 5 minutes, take the supernatant, add sterilized water to resuspend the spores, shake well and then centrifuge again;

[0048] (4) Suspend with 30% glycerol, mix thoroughly, dispense into 1.5mL / 2mL centrifuge tubes, and mark the name and time;

[0049] (5) Get a tube of spore liquid and carry out viable count, the concentration of spore liquid is 10.8×10 8 cfu / mL.

Embodiment 3

[0050] Embodiment 3: Trichoderma viride Tv-1511 spore liquid promotes seed germination effect test

[0051] 3.1. Test materials

[0052] Microorganism for testing: Trichoderma viride Tv-1511 spore liquid (prepared in embodiment 2);

[0053] Plants tested: wheat (Jimai 22) and cucumber (Jinyan 4).

[0054] 3.2. Experimental design

[0055] The germination test was carried out in the Industrial Microbiology Laboratory of the Institute of Biology, Shandong Academy of Sciences. Select healthy and plump seeds and sterilize with 75% alcohol for 30 seconds, then sterilize the surface with 2% NaClO for 3 minutes, rinse with sterile water for 4-5 times, and air-dry in an ultra-clean workbench until the surface of the seeds is dry. Choose a Petri dish with a diameter of 13 cm, 3 layers of sterile filter paper, 20 seeds per dish, and 10 mL of the corresponding germination solution. Be the control group (CK) with sterile water as the germination solution, to add Trichoderma spore liqu...

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Abstract

The disclosure belongs to the technical field of microbial growth promotion, and in particular relates to Trichoderma viride, bacterial agent, biological fertilizer and application thereof. Bio-organic fertilizers use industrial waste as raw materials to effectively solve the problem of environmental pollution through functional microorganisms, and at the same time have the effects of reducing crop diseases and increasing yields. Trichoderma bio-organic fertilizer has multiple functions such as growth promotion, production increase, and disease prevention, and has good development significance. The disclosure provides a strain of Trichoderma viride (Trichoderma viride) Tv‑1511, which has a good effect of promoting seed germination and seedling growth, and the solid fermentation product obtained by inoculating it in mushroom dregs can be used as a good bio-organic fertilizer, The utility model has the advantages of convenient use, remarkable effect of promoting production and increasing production, and has a good development prospect.

Description

technical field [0001] The disclosure belongs to the technical field of microbial growth promotion, and in particular relates to a Trichoderma viride Tv-1511 strain with a growth promotion effect, a corresponding microbial agent, a multifunctional bio-organic fertilizer prepared therefrom and applications thereof. Background technique [0002] The information disclosed in this Background section is only intended to increase the understanding of the general background of the disclosure, and is not necessarily to be taken as an acknowledgment or any form of suggestion that the information constitutes prior art that is already known to those skilled in the art. [0003] As an important means of agricultural production, fertilizer plays a vital role in food production. my country is a big agricultural country, as well as a big country in the production and consumption of chemical fertilizers. At present, the utilization rate of chemical fertilizers in a single season in my coun...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/30A01P21/00C05F5/00C05F11/08C05F17/00C12R1/885
CPCC12N1/14C05F5/00C05F11/08C05F17/00C12R2001/885C12N1/145A01N63/30Y02A40/20Y02W30/40
Inventor 郭凯黄艳华李哲郝永任隋永辉张豪
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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