Preparation method of animal glue for improving hair loss

A technology of animal glue and yak skin, which is applied in the field of preparation of small molecule peptides and keratin, can solve problems such as ignoring the effect of yak hair, and achieve the effect of easy implementation and simple method

Pending Publication Date: 2019-09-13
CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the research on yak skin has been carrie...

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1 A method for preparing animal glue for improving hair loss, comprising the following steps:

[0028] (1) Clean the fresh Qinghai-Tibet Plateau yak hide with hairs 1-2 cm away from the hide, and irradiate it with an ultraviolet lamp at a wavelength of 275 nm for 2.0 hours to obtain irradiated yak hide.

[0029] (2) The irradiated yak hide is cut into 3cm pieces, extracted in boiling water for 30 minutes, then taken out, and the yak hide is pierced with a prickly board.

[0030] (3) Put the yak hide obtained in step (2) into the enzymatic hydrolysis solution with a mass concentration of 10%, enzymolyze it at 65° C. for 36 hours, and filter to obtain the hydrolyzed yak hide and the first enzymolysis solution respectively.

[0031] Among them: the enzymolysis solution with a mass concentration of 10% refers to a solution obtained by adding 100 g of protease to 1 L of deionized water and mixing them uniformly. Protease refers to the mixture obtained by uniforml...

Embodiment 2

[0040] Embodiment 2 A preparation method of animal glue for improving hair loss, comprising the following steps:

[0041] (1) Clean the fresh Qinghai-Tibet Plateau yak skin with hairs 1-2 cm away from the skin, and irradiate it with an ultraviolet lamp at a wavelength of 200 nm for 0.3 hours to obtain irradiated yak skin.

[0042] (2) The irradiated yak hide is cut into 1cm pieces, extracted in boiling water for 5 minutes, then taken out, and the yak hide is pierced with a prickly board.

[0043] (3) Put the yak hide obtained in step (2) into the enzymolysis solution with a mass concentration of 0.5%, enzymolyze it at 30° C. for 0.5 hour, and filter to obtain the enzymolysis yak hide and the first enzymolysis solution respectively.

[0044] Among them: the enzymolysis solution with a mass concentration of 0.5% refers to a solution obtained by adding 5 g of protease to 1 L of deionized water and mixing them uniformly. Protease refers to the mixture obtained by uniformly mixing...

Embodiment 3

[0053]Embodiment 3 A preparation method of animal glue for improving hair loss, comprising the following steps:

[0054] (1) Clean the fresh Qinghai-Tibet Plateau yak hide with hairs 1-2 cm away from the hide, and irradiate it with an ultraviolet lamp at a wavelength of 223 nm for 1.0 hour to obtain irradiated yak hide.

[0055] (2) The irradiated yak hide is cut into 2cm pieces, extracted in boiling water for 15 minutes, then taken out, and the yak hide is pierced with a prickly board.

[0056] (3) Put the yak hide obtained in step (2) into the enzymatic hydrolysis solution with a mass concentration of 5%, enzymolyze it at 40° C. for 4 hours, and filter to obtain the hydrolyzed yak hide and the first enzymolysis solution respectively.

[0057] Among them: the enzymolysis solution with a mass concentration of 5% refers to a solution obtained by adding 50 g of protease to 1 L of deionized water and mixing them uniformly. Protease refers to the mixture obtained by uniformly mix...

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PUM

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Abstract

The invention relates to a preparation method of animal glue for improving hair loss. The preparation method comprises the following steps of (1) thoroughly cleaning fresh Qinghai-Tibet plateau yak skin having hair, and performing illumination through an ultraviolet lamp so as to obtain illuminated yak skin; (2) cutting the illuminated yak skin into blocks, performing extraction in boiling water,taking out the yak skin blocks after extraction, and cutting through the yak skin with a multi-stab board; (3) putting the cut-through yak skin in enzymatic hydrolysate of 0.5-10% for enzymolysis, andperforming filtration so as to obtain yak skin after enzymolysis and a first-time enzymolysis solution; (4) adding the yak skin after enzymolysis into water, and performing boiling so as to obtain filtrate; (5) adding activated carbon to the filtrate, performing sufficient stirring, then performing standing, and performing filtration so as to obtain filter residues; (6) putting the filter residues in enzymatic hydrolysate of 0.5-8% for enzymolysis, and performing filtration so as to obtain a second-time enzymolysis solution; (7) merging the first-time enzymolysis solution with the second-timeenzymolysis solution, performing boiling, and performing separation so as to obtain supernatant; and (8) filtrating the supernatant with a polyethersulfone membranes, cutting off keratin liquid of which the molecular weight is 40-75kDa and yak skin micromolecule peptide of which the molecular weight is 5kDa or below, and performing freeze drying so as to obtain yak glue. The method disclosed by the invention is simple and easy to implement.

Description

technical field [0001] The invention relates to a preparation process of small molecule peptide and keratin, in particular to a preparation method of animal glue for improving hair loss. Background technique [0002] Hair is an external manifestation of physical condition. According to the theory of traditional Chinese medicine, the kidney stores essence, the liver governs blood, and its flower is in the hair. If the body is malnourished and has abnormal metabolism, the hair follicles will not receive sufficient nutrition, resulting in hair loss. The main component of hair is keratin, which is composed of protein, iron, cystine and cysteine ​​and dozens of trace elements. Hair nutrition comes from hair follicle tissue. Collagen can improve the living environment of hair follicle cells and promote the metabolism of new hair tissue, increase microcirculation, increase hair elasticity, and reduce hair breakage. [0003] On January 31, 2019, Japanese researchers reported in the...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/47C07K1/34A61P17/14
CPCC12P21/06C07K14/4741A61P17/14
Inventor 杜玉枝杨红霞肖远灿李岑张明毕宏涛魏立新
Owner CHINA ACAD OF SCI NORTHWEST HIGHLAND BIOLOGY INST
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