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med12 gene mutation detection kit and its application

A kit and gene technology, applied in the direction of recombinant DNA technology, microbial determination/inspection, DNA/RNA fragment, etc., to achieve the effect of simple detection method

Active Publication Date: 2022-06-14
PEOPLES HOSPITAL PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no research report on MED12 mutation and its exact tumor-promoting effect in AML.

Method used

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  • med12 gene mutation detection kit and its application
  • med12 gene mutation detection kit and its application
  • med12 gene mutation detection kit and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098] Embodiment 1, the kit for detecting MED12 gene mutation and its detection method

[0099] 1. Kit for detecting MED12 gene mutation

[0100] 1. Probe sequence

[0101] The kit for detecting MED12 gene mutation of the present invention includes 48 probe sequences, and the names and specific sequences of the 48 probe sequences are shown in Table 1. The 48 probes of the present invention can cover the entire coding sequence of the MED12 gene.

[0102] Probe sequence in table 1, detection kit of the present invention

[0103]

[0104]

[0105] 2. Target area detection principle

[0106] The genomic DNA of the sample to be tested was randomly broken into fragments with a length of 200-300bp by a Covaris ultrasonic breaker, and after end repair and A-tailing, adapters were connected to both ends of the fragments to prepare a DNA library. The probe in step 1 was biotin-labeled, and then the DNA library with a specific index was hybridized with the biotin-labeled probe...

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Abstract

The invention discloses a MED12 gene mutation detection kit and an application thereof. The kit of the present invention includes a probe set for detecting MED12 gene mutation, and the probe set is composed of probe 1-probe 48. The kit of the present invention can not only be used to detect MED12 somatic mutation and germline mutation at the level of tumor genome, but also can be used for auxiliary diagnosis, monitoring of minimal residual disease and evaluation of prognosis in patients with hematological tumors, especially acute myeloid leukemia patients. , and the detection method of the kit of the present invention is simple, sensitive and accurate, and will play an important role in the field of medical detection.

Description

technical field [0001] The invention belongs to the technical field of medical detection, and in particular relates to a MED12 gene mutation detection kit and application thereof. Background technique [0002] Acute myeloid leukemia (AML) is a malignant clonal disease originating from hematopoietic stem cells with high molecular biology and clinical heterogeneity. The development of molecular diagnostic technology has played a huge role in the precise classification and individualized treatment of AML, but despite this, the clinical prognosis of AML patients still shows huge heterogeneity, and recurrence is still common among clinicians and AML patients. One of the challenges we face, and the discovery of new biomarkers is the only way to realize the individualized diagnosis and treatment of AML. [0003] MED12 (mediator complex subunit 12) is located on the X sex chromosome and encodes the mediator complex subunit 12 protein. It forms a four-subunit kinase module together ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12Q1/6858C12N15/11
CPCC12Q1/6886C12Q1/6858C12Q2600/156C12Q2535/122
Inventor 阮国瑞黄晓军周亚兰吴利新王子龙
Owner PEOPLES HOSPITAL PEKING UNIV
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