A bacterial strain producing lactic acid by high-temperature fermentation and a method for producing lactic acid

A high-temperature fermentation and lactic acid technology, applied in the field of bioengineering, can solve problems such as fermentation and production of lactic acid, and achieve the effects of good fermentation stability, improved adaptability, and improved efficiency

Active Publication Date: 2021-12-21
DALIAN UNIV OF TECH
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  • Claims
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Problems solved by technology

[0009] In order to overcome the problem that the existing Enterococcus faecalis cannot ferment and produce lactic acid at a temperature of 50°C or even higher than 50°C, the present invention provides a strain for producing lactic acid by high-temperature fermentation and a method for producing lactic acid by fermenting the strain

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  • A bacterial strain producing lactic acid by high-temperature fermentation and a method for producing lactic acid
  • A bacterial strain producing lactic acid by high-temperature fermentation and a method for producing lactic acid
  • A bacterial strain producing lactic acid by high-temperature fermentation and a method for producing lactic acid

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Screening preparation and identification of E.faecalis DUT1805:

[0052] Take 0.1kg of the fresh content of bovine stomach in a Erlenmeyer flask with 100mL of normal saline, vortex for 2min, and inoculate the solid-liquid mixture into a vial with 100mL of enrichment medium for anaerobic enrichment culture. After 12 consecutive subcultures, the mixed bacteria with stable fermentation performance were obtained. The flora was inoculated into a 250mL vial containing 100mL RCM medium, heat-treated at 90°C for 20 minutes, and then spread on an RCM plate. After the plate was anaerobically cultured at 37°C for 24 hours, a single colony was picked for streaking and isolation, and then purified for 3 Conservation of species for future generations. The isolated and purified single bacterial DNA was extracted and amplified with 16S rDNA bacterial general primers (primer 27F: AGTTTGATCMTGGCTCAG; 1492R: GGTTACCTTGTTACGACTT). Through comparative analysis of 16S rDNA, it was confirmed...

Embodiment 2

[0053] Example 2 Effect of glucose stress on the fermentation performance of E.faecalis DUT1805 at 50°C

[0054] After Enterococcus faecalis DUT1805 passed through the seed medium, it was inoculated into a 5L fermenter with 2LMRS medium at a volume ratio of 5%, and the temperature was gradually raised from 37°C to 50°C within 2 hours, and the glucose concentration was increased from 40g / L is gradually increased to 160g / L, the whole fermentation process is not ventilated, and the fermentation results are as follows: image 3 shown. The results showed that Enterococcus faecalis DUT1805 could tolerate 160g / L glucose at high temperature of 50℃. Among them, the batch fermentation efficiency is the highest when the glucose concentration is 40g / L, the final concentration of lactic acid is 37.92g / L, the conversion rate relative to glucose is 0.92g / g, the production intensity is 3.79g / (L.h), and the metabolic by-products There are acetic acid, succinic acid and ethanol, the concentr...

Embodiment 3

[0055] Example 3 Effects of different temperature control strategies on the fermentation performance of E.faecalis DUT1805 at 50°C

[0056] Enterococcus faecalis DUT1805 was inoculated into a 5L fermenter with 2L MRS medium at a volume ratio of 5% after the seed medium, and the whole fermentation process was not ventilated. To explore the effect of different gradient heating methods on E.faecalis DUT1805 using glucose fermentation Effects on the production of lactic acid. Fermentation results such as Figure 4 shown. When the substrate was 40g / L glucose, the experimental group whose temperature was raised from 37°C to 50°C within 4 hours had better cell growth, glucose consumption rate and lactic acid production rate than the experimental group whose temperature was gradually raised to 50°C within 2h and 6h. The final concentration of lactic acid was 36.56g / L, the conversion rate relative to glucose was 0.92g / g, the production intensity was 4.57g / (L.h), and the concentration...

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Abstract

The present invention provides a bacterial strain for producing lactic acid by high-temperature fermentation and a method for producing lactic acid. The bacterial strain is named: E.faecalis DUT1805, and the bacterial strain is used to produce lactic acid by high-temperature fermentation of glucose in a gradient heating mode, and the gradient temperature is 37-40-45 -50°C gradient or 37-40-45-50°C-55°C gradient, from 37°C to 50°C or 55°C, the time required for the overall temperature rise is 2h, 4h, 6h or 8h. E.faecalis DUT1805 has high production intensity and lactic acid conversion rate; at the same time, E.faecalis DUT1805 can directly metabolize biomass raw materials such as rice flour or corn stalk hydrolyzate to produce lactic acid at a high temperature of 50°C without gradient heating.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a bacterial strain for producing lactic acid by high-temperature fermentation and a method for producing lactic acid by using the bacterial strain. Background technique [0002] Lactic acid, also known as α-hydroxypropionic acid, is one of the three major organic acids (carboxylic acid, hydroxy acid, keto acid), an important fine chemical, and one of the 12 platform compounds for the petroleum substitution strategy determined by the US Department of Energy One (Zhang Li, Bioprocessing, 2018, 16(4):23-29). Lactic acid is widely used in food, chemical, medical and other fields. Among them, the synthesis of polylactic acid with lactic acid as a monomer is the most promising application of lactic acid (Andrea Komesu et al., BioResources, 2017, 12(2):4364-4383) . With the depletion of non-renewable resources such as petroleum resources and the deterioration of the...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P7/56C12R1/01
CPCC12P7/56C12R2001/01C12N1/205
Inventor 孙亚琴刘慧慧徐振振修志龙
Owner DALIAN UNIV OF TECH
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