A kit for detecting Enterobacter cloacae

Abstract

The invention provides a kit for the detection of Enterobacter cloacae, including guide RNAs specifically targeting the rpoB gene of the Enterobacter cloacae, a primer pair for amplification, hydrated TwistAmp basic kit reaction drying balls, LbCas12a proteins, a single-stranded DNA probe (ssDNA), Ribonuclease Inhibitor and buffer. The kit can be used to detect the Enterobacter cloacae with high specificity, and can distinguish the Enterobacter cloacae from other Enterobacter bacteria, including Enterobacter aerogenes, Enterobacter gergoviae and Enterobacter sakazakii. At the same time, the method using the RNA sequence described in the invention for detection takes about 1 hour, does not need any PCR instruments, and has low requirements for equipment; and time consumption of the method is significantly lower than that of the conventional bacterial culture method or the PCR sequencing method, with great clinical practical value.

Description

technical field [0001] The invention belongs to the field of detection of pathogenic bacteria, and relates to a kit for detection of Enterobacter cloacae. This kit can be used for rapid detection of Enterobacter cloacae. Background technique [0002] Enterobacter cloacae is a common bacterial group among Gram-negative bacteria that infects human beings, which brings a huge burden to patients and society. The traditional detection method is bacterial culture or combined PCR sequencing, but bacterial culture takes 5-7 days, and PCR often cannot distinguish Enterobacter cloacae from other Enterobacter bacteria due to the lack of specific primers, so combined generation is required Sequencing can only be identified, the cost is high, and it takes several days to get the results. During this period, clinicians can only use medicines based on experience because they do not obtain test results, which often leads to delays in disease treatment. Therefore, how to quickly detect En...

AI Technical Summary

Problems solved by technology

The traditional detection method is bacterial culture or combined PCR sequencing, but bacterial culture takes 5-7 days, and PCR often cannot distinguish Enterobacter cloacae from other Enterobacter bacteria due to the lack of specific primers, so combined generation is required Sequencing can only be identified, the cost is high, and it takes several days to get the result

During this period, clinicians can only use medicines based on experience because they have not obtained test results, which often leads to delays in disease treatment

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