Method of assessing heavy metal polluted soil ecotoxicity through local soil test animal

Abstract

The invention discloses a method of assessing heavy metal polluted soil ecotoxicity through a local soil test animal. According to the method, fridericia bulbosa is selected to serve as a test animal; before a formal test, a trial test is performed first, wherein a certain quantity of the fridericia bulbosa is placed in soil in different heavy metal concentration treatment groups, the water content of the soil is controlled at 30-45%, the soil is placed in an artificial climate box under the temperature of 15-25 DEG C, the humidity of 70-80% and illumination for the trial test; in reference to the lowest lethal concentration determined in the trial test result, a heavy metal exposure concentration in the formal test is set; and the fridericia bulbosa in the same quantity as those in the trial test is placed in each concentration treatment group, the survival condition of the fridericia bulbosa in each concentration treatment group after exposure for a certain time is recorded, and an LD50 value is calculated. Through the method, the fridericia bulbosa is selected to serve as the test animal to assess the heavy metal polluted soil ecotoxicity, all parameters in the test process are quantified, and it is convenient to carry out the test; and operation is convenient and easy, consumed time is short, and sensitivity is high.

Description

technical field [0001] The invention belongs to the technical field of ecological toxicity assessment of polluted soil, and relates to a method for assessing the ecological toxicity of heavy metal polluted soil by using localized soil test animal Elephria sphaeroides. Background technique [0002] Soil is an important natural environment for human survival. With the rapid development of industry, the problem of heavy metal pollution in soil has become increasingly prominent, and soil pollution restoration has received increasing attention. It is of great significance to study sensitive ecological toxicity assessment methods of polluted soils for evaluating polluted soils and the ecological environment after remediation. [0003] At present, reports on the assessment methods of heavy metal pollution in soil mainly use physical or chemical methods to assess the total amount of heavy metals in the soil, and set limits. There are few reports on ecological toxicity assessment. In...

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Problems solved by technology

[0003] At present, reports on the assessment methods of heavy metal pollution in soil mainly use physical or chemical methods to assess the total amount of heavy metals in the soil, and set limits. There are few reports on ecological toxicity assessment. In addition, Qiu Jiangping reported using earthworms to assess pollution Soil ecotoxicity has certain environmental, economic and social benefits

However, these existing soil ecotoxicity assessment methods have the disadvantages of long operation period, poor reproducibility and low sensitivity.