Method of assessing heavy metal polluted soil ecotoxicity through local soil test animal

A technology for testing animals and ecotoxicity, applied in soil material testing, material inspection products, etc., can solve the problems of less ecotoxicity assessment, long operation period, poor reproducibility, etc. strong effect

Pending Publication Date: 2019-09-17
SHANGHAI ACADEMY OF ENVIRONMENTAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, reports on the assessment methods of heavy metal pollution in soil mainly use physical or chemical methods to assess the total amount of heavy metals in the soil, and set limits. There are few reports on ecological toxicity assessment. In addition, Qiu Jia

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 A method for evaluating the ecotoxicity of arsenic-contaminated soil by using the localized soil test animal, Altus sphaeroides

[0034] 1. Test steps:

[0035] 1. The selection of experimental animals (Lepis sphaeroides)

[0036] A kind of white-line worm, namely Fridericia bulbosa, was selected as the experimental animal to carry out the ecotoxicity effect test of heavy metals on the heavy metal-contaminated soil. Robust adults with annulus were tested at ±0.2 mg (mean ± standard deviation).

[0037] 2. Pre-test

[0038] Before the formal test, a pre-test with arsenic concentration of 0.1, 1.0, 10, 100, 1000mg / kg soil dry weight was carried out. Mix 200mL of heavy metal deionized aqueous solutions of different concentrations with 500g of artificial soil (50g of sphagnum moss, 100g of kaolin clay, and 350g of quartz sand) to make the water content of the soil reach 40%, and put it in a 1L beaker. Put 100 worms into the soil of each test in each concentrat...

Embodiment 2

[0051] Example 2 A method for assessing the ecotoxicity of cadmium-contaminated soil by using the localized soil test animal, Altus sphaeroides

[0052] 1. Test steps:

[0053] 1. The selection of experimental animals (Lepis sphaeroides)

[0054] A kind of white-line worm, namely Fridericia bulbosa, was selected as the experimental animal to carry out the ecotoxicity effect test of heavy metals on the heavy metal-contaminated soil. Robust adults with annulus were tested at ±0.2 mg (mean ± standard deviation).

[0055] 2. Pre-test

[0056] Before the formal test, a pre-test with cadmium concentration of 0.1, 1.0, 10, 100, 1000 mg / kg soil dry weight was carried out. Mix 200mL of heavy metal deionized aqueous solutions of different concentrations with 500g of artificial soil (50g of sphagnum moss, 100g of kaolin clay, and 350g of quartz sand) to make the water content of the soil reach 40%, and put it in a 1L beaker. Put 100 worms into the soil of each test in each concentrat...

Embodiment 3

[0069] Example 3 A method for evaluating the ecotoxicity of mercury-contaminated soil by using the localized soil test animal, Altus sphaeroides

[0070] 1. Test steps:

[0071] 1. The selection of experimental animals (Lepis sphaeroides)

[0072] A kind of white-line worm, namely Fridericia bulbosa, was selected as the experimental animal to carry out the ecotoxicity effect test of heavy metals on the heavy metal-contaminated soil. Robust adults with annulus were tested at ±0.2 mg (mean ± standard deviation).

[0073] 2. Pre-test

[0074] Before the formal test, a preliminary test with mercury concentrations of 0.1, 1.0, 10, 100, 1000mg / kg soil dry weight was carried out. Mix 200mL of heavy metal deionized aqueous solutions of different concentrations with 500g of artificial soil (50g of sphagnum moss, 100g of kaolin clay, and 350g of quartz sand) to make the water content of the soil reach 40%, and put it in a 1L beaker. Put 100 worms into the soil of each test in each c...

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PUM

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Abstract

The invention discloses a method of assessing heavy metal polluted soil ecotoxicity through a local soil test animal. According to the method, fridericia bulbosa is selected to serve as a test animal; before a formal test, a trial test is performed first, wherein a certain quantity of the fridericia bulbosa is placed in soil in different heavy metal concentration treatment groups, the water content of the soil is controlled at 30-45%, the soil is placed in an artificial climate box under the temperature of 15-25 DEG C, the humidity of 70-80% and illumination for the trial test; in reference to the lowest lethal concentration determined in the trial test result, a heavy metal exposure concentration in the formal test is set; and the fridericia bulbosa in the same quantity as those in the trial test is placed in each concentration treatment group, the survival condition of the fridericia bulbosa in each concentration treatment group after exposure for a certain time is recorded, and an LD50 value is calculated. Through the method, the fridericia bulbosa is selected to serve as the test animal to assess the heavy metal polluted soil ecotoxicity, all parameters in the test process are quantified, and it is convenient to carry out the test; and operation is convenient and easy, consumed time is short, and sensitivity is high.

Description

technical field [0001] The invention belongs to the technical field of ecological toxicity assessment of polluted soil, and relates to a method for assessing the ecological toxicity of heavy metal polluted soil by using localized soil test animal Elephria sphaeroides. Background technique [0002] Soil is an important natural environment for human survival. With the rapid development of industry, the problem of heavy metal pollution in soil has become increasingly prominent, and soil pollution restoration has received increasing attention. It is of great significance to study sensitive ecological toxicity assessment methods of polluted soils for evaluating polluted soils and the ecological environment after remediation. [0003] At present, reports on the assessment methods of heavy metal pollution in soil mainly use physical or chemical methods to assess the total amount of heavy metals in the soil, and set limits. There are few reports on ecological toxicity assessment. In...

Claims

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Application Information

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IPC IPC(8): G01N33/24
CPCG01N33/24
Inventor 朱江李炳智杨道丽杨洁张施阳吉敏余强
Owner SHANGHAI ACADEMY OF ENVIRONMENTAL SCIENCES
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