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Preparation method of chitosan/sodium tripolyphosphate microsphere slow-release system with embedded GDNF (glial cell-derived neurotrophic factor)

A technology of sodium tripolyphosphate and chitosan microspheres, which can be applied to nervous system diseases, medical preparations containing no active ingredients, medical preparations containing active ingredients, etc. Low ball rate and other problems, to achieve the effect of reducing burst release, good drug sustained release performance, and high ball forming rate

Pending Publication Date: 2019-09-20
XIAN HONGHUI HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a preparation method of GDNF-embedded chitosan / sodium tripolyphosphate microsphere slow-release system, which solves the problems of poor mechanical properties of microspheres, low spheroidization rate, and inability to produce microspheres in the prior art. Effectively maintain the spherical structure, but lose the good drug sustained release effect; at the same time protect the biological activity of GDNF and improve the bioavailability of GDNF

Method used

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  • Preparation method of chitosan/sodium tripolyphosphate microsphere slow-release system with embedded GDNF (glial cell-derived neurotrophic factor)
  • Preparation method of chitosan/sodium tripolyphosphate microsphere slow-release system with embedded GDNF (glial cell-derived neurotrophic factor)
  • Preparation method of chitosan/sodium tripolyphosphate microsphere slow-release system with embedded GDNF (glial cell-derived neurotrophic factor)

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Effect test

Embodiment 1

[0070] The preparation method of the GDNF-embedded chitosan / sodium tripolyphosphate microsphere slow-release system of the present invention is specifically implemented according to the following steps:

[0071] Step 1, prepare the aqueous phase:

[0072] First weigh 200mg of chitosan powder, fully dissolve it in 10ml of glacial acetic acid aqueous solution and let it stand, filter to remove impurities to obtain a transparent chitosan solution;

[0073] Add 4 mg of bovine serum albumin, 2 ml of fetal bovine serum, and 20 μg of GDNF into 1 ml of phosphate buffered saline solution to obtain a GDNF solution;

[0074] Add the GDNF solution into the chitosan solution, and mix well to obtain a chitosan solution containing GDNF;

[0075]Among them, when preparing the water phase, the ambient temperature was controlled at 6°C, the concentration of glacial acetic acid was 15% w / v%, the chitosan powder was fully dissolved in the aqueous glacial acetic acid solution, and the standing ti...

Embodiment 2

[0087] The preparation method of the GDNF-embedded chitosan / sodium tripolyphosphate microsphere slow-release system of the present invention is specifically implemented according to the following steps:

[0088] Step 1, prepare the aqueous phase:

[0089] First weigh 300mg of chitosan powder, fully dissolve it in 20ml of glacial acetic acid aqueous solution and let it stand, filter to remove impurities to obtain a transparent chitosan solution;

[0090] Add 2mg of bovine serum albumin, 2ml of fetal bovine serum, and 40μg of GDNF into 2ml of phosphate-buffered saline to obtain a GDNF solution;

[0091] Add the GDNF solution into the chitosan solution, and mix well to obtain a chitosan solution containing GDNF;

[0092] Among them, when preparing the water phase, the ambient temperature was controlled at 4°C, the concentration of glacial acetic acid was 10% w / v%, the chitosan powder was fully dissolved in the aqueous solution of glacial acetic acid, and the standing time was 6 ...

Embodiment 3

[0104] The preparation method of the GDNF-embedded chitosan / sodium tripolyphosphate microsphere slow-release system of the present invention is specifically implemented according to the following steps:

[0105] Step 1, prepare the aqueous phase:

[0106] First weigh 200mg of chitosan powder, fully dissolve it in 20ml of glacial acetic acid aqueous solution and let it stand, filter to remove impurities to obtain a transparent chitosan solution;

[0107] Add 4 mg of bovine serum albumin, 2 ml of fetal bovine serum, and 30 μg of GDNF into 2 ml of phosphate buffered saline solution to obtain a GDNF solution;

[0108] Add the GDNF solution into the chitosan solution, and mix well to obtain a chitosan solution containing GDNF;

[0109] Among them, when preparing the water phase, the ambient temperature was controlled at 4°C, the concentration of glacial acetic acid was 11% w / v%, the chitosan powder was fully dissolved in the aqueous solution of glacial acetic acid, and the standin...

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Abstract

The invention discloses a preparation method of a chitosan / sodium tripolyphosphate microsphere slow-release system with embedded GDNF (glial cell-derived neurotrophic factor). The preparation method comprises: preparing a chitosan solution with chitosan and glacial acetic acid solution; adding bovine serum albumin, fetal calf serum and GDNF into a phosphate solution to prepare a GDNF solution; adding the GDNF solution into the chitosan solution to prepare a GDNF chitosan solution; adding the GDNF chitosan solution into liquid paraffin with Span 80, and performing mechanical stirring to obtain a chitosan microsphere solution with embedded GDNF; and adding sodium tripolyphosphate solution into the chitosan microsphere solution with embedded GDNF, and performing mechanical stirring to obtain the chitosan / sodium tripolyphosphate microsphere slow-release system with embedded GDNF. The problem of the prior art is solved that chitosan microspheres have poor mechanical property, low pelletizing rate and poor slow-release stability; bioactivity of GDNF is protected, bioavailability of the GDNF is improved, and neural regeneration is promoted.

Description

technical field [0001] The invention belongs to the technical field of macromolecule biomaterials, and in particular relates to a preparation method of a GDNF-embedded chitosan / sodium tripolyphosphate microsphere slow-release system. Background technique [0002] As we all know, peripheral nerve injury often leads to motor and sensory dysfunction in patients, seriously affects the quality of life of patients, and brings great pain and heavy economic burden to families and society. The regeneration of peripheral nerve after injury is a dynamic repair process involving a variety of cells and factors, in which neurotrophic factors play a vital role in this repair process. In the first few days after nerve injury, macrophages and Schwann cells come together and participate in the phagocytosis of axonal and myelin sheath debris. Subsequently, the newly proliferated Schwann cells line up along the endoneurial tube, and together with the remaining Schwann cells at the nerve injury...

Claims

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Application Information

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IPC IPC(8): A61K38/18A61K9/52A61K47/36A61K47/02A61P25/00
CPCA61K38/185A61K9/5036A61K9/501A61P25/00
Inventor 曾文郝定均贺宝荣惠华
Owner XIAN HONGHUI HOSPITAL
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