Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Molecular marker for identifying hybrid broussonetia papyrifera and application of molecular marker

A molecular marker and tree-building technology, which is applied in the detection/testing of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., to achieve the effect of guaranteeing interests and broad application prospects

Active Publication Date: 2019-09-20
中科创构(北京)科技有限公司
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no DNA molecular marker specifically for hybrid trees on the market

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Molecular marker for identifying hybrid broussonetia papyrifera and application of molecular marker
  • Molecular marker for identifying hybrid broussonetia papyrifera and application of molecular marker
  • Molecular marker for identifying hybrid broussonetia papyrifera and application of molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] Example 1. Molecular markers for identification of hybridization trees and the design of specific amplification primer pairs

[0073] 1. Molecular markers of hybrid tree

[0074] The present invention provides a DNA barcode (DNA barcode) derived from a hybrid tree (Broussonetia kazinoki×B.papyrifera), the nucleotide sequence of which is shown in sequence 3 in the sequence listing, and it is named Bpmcbode. The DNA molecule shown in sequence 3 is the molecular marker used to identify the hybrid tree.

[0075] 2. Specific primer pairs

[0076] According to the Bpmcbode sequence unique to the hybrid tree obtained in step 1, a primer pair for specific amplification of its full length is designed, and the primer sequence is as follows:

[0077] F: 5'-AACCTGGGGCGTATTGCTCAA-3' (SEQ ID NO: 1);

[0078] R: 5'-TTACCATATATATATAACACAAAATTTC-3' (SEQ ID NO: 2).

Embodiment 2

[0079] Embodiment 2, the specific detection that is used for identifying the primer pair of hybridization tree

[0080] 1. DNA extraction from plant material

[0081] The total DNA of each plant material in Table 1 was extracted respectively, and the extracted total DNA was detected by 1% agarose gel electrophoresis. Among them, the agarose gel electrophoresis detection results of the total DNA extracted from the leaves, roots or stems of the hybrid tree are as follows: figure 1 shown. The results showed that the extracted DNA had obvious electrophoresis bands, which were larger than 20kb, indicating that a relatively high-purity and relatively complete DNA was obtained.

[0082] 2. PCR amplification

[0083] The total DNA sample extracted in step 1 was used as a template, and the primer pair designed in step 2 of Example 1 was used for PCR amplification to obtain PCR products respectively.

[0084] The PCR reaction system was as follows (total volume: 25 μL): DNA template...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a molecular marker for identifying hybrid broussonetia papyrifera and application of the molecular marker. The molecular marker refers to 1) a DNA molecule shown as a sequence 3 in a sequence table and 2) a DNA molecular functionally identical to the sequence 3 and obtained by subjecting the sequence 3 to substitution and / or deficiency and / or adding of one or more nucleotides. The molecular marker derived from hybrid broussonetia papyrifera can be applied to distinguishing of hybrid broussonetia papyrifera from other plants such as wild broussonetia papyrifera and broussonetia Kazinoki, has important theoretical and practical significant to variety identification of the hybrid broussonetia papyrifera, can be used for cultivation and identification of plant varieties required by husbandry and ecological environment management and has a high practical application value. The molecular marker has a promising application prospect in the field of agriculture and industrial energy crops.

Description

technical field [0001] The invention relates to a plant molecular marker, in particular to a molecular marker for identifying a hybrid tree and its application. Background technique [0002] DNA molecular identification can be applied to paternity identification, disease diagnosis, prenatal screening, identification of Chinese herbal medicine, identification of plant varieties, identification of genetically modified genes, etc. DNA molecular identification is a new biological identification system created by using standard, sufficiently variable, easy-to-amplify and relatively short DNA fragments to create their own specificity within species and diversity among species. Rapid automatic identification of species or samples is possible. [0003] Broussonetia papyrifera (Broussonetia papyrifera), also known as mulberry tree, mulberry tree, glaucous mulberry tree, mulberry peach, corn pulp tree, deer tree, Dangdang tree, milk tree, paper wood, belongs to nitrogen fixation in t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895
Inventor 沈世华彭献军
Owner 中科创构(北京)科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com