Method for determining N-nitrosamines compounds in meat product by adopting GPC-GC/MS/MS

A technology of nitrosamines and compounds, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of cumbersome, difficult to realize batch sample processing, complicated operation, etc., achieve convenient operation, realize efficient processing of batch samples, and high sensitivity Effect

Active Publication Date: 2019-09-20
南通市产品质量监督检验所
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Problems solved by technology

The method standard for the detection of N-nitrosamines is GB 5009.26-2016 "National Food Safety Standard for the Determination of N-nitrosamine Compounds in Food", which involves gas chromatography-mass spectrometry and gas chromatography-thermal energy analysis The two methods involved in the pretreatment process include steam distillation extraction, liquid-liquid extraction purification, concentration treatment, etc. The operation is relatively complicated and cumbersome, and it is difficult to achieve batch sample processing

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  • Method for determining N-nitrosamines compounds in meat product by adopting GPC-GC/MS/MS
  • Method for determining N-nitrosamines compounds in meat product by adopting GPC-GC/MS/MS
  • Method for determining N-nitrosamines compounds in meat product by adopting GPC-GC/MS/MS

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Embodiment

[0057] 1. Standard spectra and curve equations of N-nitrosamine compounds

[0058] Nine kinds of N-nitrosamine compound standard solutions are diluted step by step into 2 μg / L, 5 μg / L, 10 μg / L, 50 μg / L, 100 μg / L, 200 μg / L, 500 μg / L series standard solutions, using the present invention The chromatographic conditions and mass spectrometry parameters provided were analyzed, and the total ion current spectra of the standard solutions of 9 kinds of nitrosamine compounds were as follows: figure 1 shown. Taking the concentration of the standard solution as the abscissa, and the corresponding quantitative ion-pair peak area as the ordinate, the corresponding standard curve equations for each compound are obtained. The linear relationship is good, and the correlation coefficients are all greater than 0.99. For details, see Table 1.

[0059] Table 1

[0060]

[0061]

[0062] 2. Analysis of N-nitrosamine compounds in fragrant powder sausage

[0063] Crush the meat product sam...

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Abstract

The present invention provides a method for determining N-nitrosamines compounds in a meat product by adopting GPC-GC/MS/MS. The method comprises the following steps of: (1) ultrasonic extraction; (2) GPC purification; (3) online concentration; (4) GC/MS/MS detection; and (5) qualitative and quantitative analysis. Pre-processing of samples is performed through the online concentration, and a gas chromatography-tandem mass spectrum is detected, the operation is convenient and rapid, the efficient processing of the samples in batch can be achieved, and moreover, the sensitivity is high, and the important meaning for the checkout of the trace constituent is provided.

Description

technical field [0001] The invention relates to a method for determining N-nitrosamine compounds in meat products by using GPC-GC / MS / MS, in particular to a detection method based on GPC pretreatment and analysis by gas chromatography-triple quadrupole tandem mass spectrometry method. Background technique [0002] N-Nitrosamine compounds are strong carcinogenic substances with teratogenic and mutagenic effects. They have various types and have the typical structure shown in the following formula. They exist in air, soil, water and food in nature. [0003] [0004] The risk of contamination of nitrosamine compounds in meat products, especially those produced by curing, smoking, baking and other processes, is very high, mainly due to the presence of precursor substances such as nitrite, nitrate and amines. Nitrite and nitrate are used as color-preserving agents and preservatives in meat processing, and protein in meat products will decompose to produce amines, which react u...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/08G01N30/14G01N30/72
CPCG01N30/02G01N30/06G01N30/08G01N30/14G01N30/72G01N2030/062
Inventor 邵彪周小兰陈煜陈刚王琳琳李玲玉沈祖建
Owner 南通市产品质量监督检验所
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